Predicted mutation
evidence seq id position mutation annotation gene description
RA AM260480 157,096 +G intergenic (‑26/+41) h16_B0136 ← / ← h16_B0137 cysteine desulfurase/conserved hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM260480157,0941.G100.0% 146.8 / NA 38intergenic (‑24/+43)h16_B0136/h16_B0137cysteine desulfurase/conserved hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (20/18);  total (20/18)

GTATCGAGCACCGCGTCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGC‑GGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCACGCCGCCGGCGAAGGCGC  >  AM260480/156961‑157213
                                                                                                                                      |                                                                                                                       
gTATCGAGCACCGCGTCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGc                                                                                                                          >  2:281240/1‑134 (MQ=255)
             cgTCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGAc                                                                                                             >  2:1600/1‑134 (MQ=255)
               tCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcg                                                                                                           >  2:46453/1‑134 (MQ=255)
               tCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcg                                                                                                           >  2:368117/1‑134 (MQ=255)
               tCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcg                                                                                                           >  2:1118001/1‑134 (MQ=255)
                 gggCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgcg                                                                                                         >  1:595137/1‑134 (MQ=255)
                 gggCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgc                                                                                                          <  1:98179/133‑1 (MQ=255)
                  ggcggcAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCgcgc                                                                                                        <  1:368117/134‑1 (MQ=255)
                       cAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGa                                                                                                   <  1:298061/134‑1 (MQ=255)
                           gAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGt                                                                                                >  2:1049638/1‑133 (MQ=255)
                            aGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTaa                                                                                              <  1:1045511/134‑1 (MQ=255)
                            aGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTaa                                                                                              <  2:595137/134‑1 (MQ=255)
                                 gcggcgGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTc                                                                                         <  1:395428/134‑1 (MQ=255)
                                 gcggcgGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTc                                                                                         <  1:1600/134‑1 (MQ=255)
                                    gcggTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGt                                                                                      <  1:1118001/134‑1 (MQ=255)
                                              gatagatGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCactta                                                                            <  1:1049638/134‑1 (MQ=255)
                                                   atGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTg                                                                                    >  1:1075524/1‑121 (MQ=255)
                                                   atGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTg                                                                                    <  2:1075524/121‑1 (MQ=255)
                                                        ggAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGc                                                                  >  2:866871/1‑134 (MQ=255)
                                                        ggAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGc                                                                  >  1:282704/1‑134 (MQ=255)
                                                        ggAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGc                                                                  >  2:295734/1‑134 (MQ=255)
                                                           ggCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgc                                                                                                          >  1:1025566/1‑91 (MQ=255)
                                                           ggCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgc                                                                                                          <  2:1025566/91‑1 (MQ=255)
                                                           ggCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTg                                                               <  1:866871/134‑1 (MQ=255)
                                                             ccGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgc                                                                                                          >  1:786782/1‑89 (MQ=255)
                                                             ccGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgc                                                                                                          <  2:786782/89‑1 (MQ=255)
                                                                 cAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACg                                                         >  2:572769/1‑134 (MQ=255)
                                                                    cGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAg                                                      <  1:281240/134‑1 (MQ=255)
                                                                     ggggTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCacttact                                                                          >  1:906228/1‑113 (MQ=255)
                                                                     ggggTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCacttact                                                                          <  2:906228/113‑1 (MQ=255)
                                                                           tCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCgaaga                                               <  2:734274/134‑1 (MQ=255)
                                                                                       gCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgc                                                                                                          >  1:560024/1‑63 (MQ=255)
                                                                                       gCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACcgc                                                                                                          <  2:560024/63‑1 (MQ=255)
                                                                                                    ggATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCacttact                                                                          >  2:144718/1‑82 (MQ=255)
                                                                                                    ggATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCacttact                                                                          <  1:144718/82‑1 (MQ=255)
                                                                                                     gATATCCATGGAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAAt                      >  1:587626/1‑133 (MQ=255)
                                                                                                               gAACGTCTCGGGTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCTATCACGCCGCCgg           >  1:961468/1‑134 (MQ=255)
                                                                                                                        gggTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCACGCCGCCGGCGAAGgcgc  >  2:489758/1‑134 (MQ=255)
                                                                                                                        gggTAATGACAGGCGGGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCACGCCGCCGGCGAAGgcgc  >  2:1087245/1‑134 (MQ=255)
                                                                                                                                      |                                                                                                                       
GTATCGAGCACCGCGTCGGGCGGCAACGAGGCCGCGGCGGTGTCGAGATAGATGGTGGAGGCCGTCAGCGGGGTGTCGGCGCGGATCGCCTGGATGGCTTGGATATCCATGGAACGTCTCGGGTAATGACAGGC‑GGACGCGGCGACCGCGCCCCGAGGTAAGACTCAGTTGCGCACTTACTTGCGCAGCTTGGTGACGGAGCCGAAGAAGCTGAACAGGGCGTCGCCGGCAATCACGCCGCCGGCGAAGGCGC  >  AM260480/156961‑157213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑