Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	159,381	+G	intergenic (‑13/‑342)	h16_B0138 ← / → h16_B0139	transcriptional regulator, IclR‑family/conserved hypothetical transmembrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	159,380	1	.	G	100.0%	142.2 / NA	42	intergenic (‑12/‑343)	h16_B0138/h16_B0139	transcriptional regulator, IclR‑family/conserved hypothetical transmembrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (19/23);  total (19/23)

CGCGCCAGCGCTTCCAGCGCCGGTTGCACCACCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTT‑GCGGCAAA‑GTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAGGGCGCCGTC  >  AM260480/159250‑159504                                                                                                                                    |                                                                                                                              cgcgcCAGCGCTTCCAGCGCCGGTTGCACCACCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTT‑ggc                                                                                                                            >  1:464518/1‑132 (MQ=255)                aGCGCCGGTTGCACCACCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                              <  1:684407/134‑1 (MQ=255)                aGCGCCGGTTGCACCACCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                              <  2:845494/134‑1 (MQ=255)                aGCGCCGGTTGCACCACCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                              <  2:305049/134‑1 (MQ=255)                aGCGCCGGTTGCACCACCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                              <  2:443366/134‑1 (MQ=255)                aGCGCCGGTTGCACCACCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                              <  1:1113540/134‑1 (MQ=255)                aGCGCCGGTTGCACCACCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                              <  2:50042/134‑1 (MQ=255)                           caccacCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGc                                                                                                    <  2:170609/133‑1 (MQ=255)                               accTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGcaccac                                                                                               <  2:464518/134‑1 (MQ=255)                                      ccggcGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCgtgg                                                                                        <  1:1152471/134‑1 (MQ=255)                                      ccggcGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCgtgg                                                                                        <  1:1100465/134‑1 (MQ=255)                                      ccggcGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCgtgg                                                                                        <  1:822440/134‑1 (MQ=255)                                      ccggcGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCgtgg                                                                                        <  1:705048/134‑1 (MQ=255)                                              aaCGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCAcgcc                                                                                >  2:619803/1‑134 (MQ=255)                                              aaCGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCAcgcc                                                                                >  1:767066/1‑134 (MQ=255)                                                                tGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTc                                                              >  2:242783/1‑134 (MQ=255)                                                                     cAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGAAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCa                                                         >  2:87209/1‑134 (MQ=25)                                                                           gAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGcc                                                                                                                <  2:1009701/73‑1 (MQ=25)                                                                           gAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGcc                                                                                                                >  1:1009701/1‑73 (MQ=25)                                                                           gAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                              >  2:789070/1‑75 (MQ=25)                                                                           gAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                              <  1:789070/75‑1 (MQ=25)                                                                                aGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCgtgg                                                                                        >  2:605389/1‑92 (MQ=21)                                                                                aGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCgtgg                                                                                        <  1:605389/92‑1 (MQ=21)                                                                                aGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAA‑gg                                                                                                                    <  2:819353/63‑2 (MQ=25)                                                                                aGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAA‑gg                                                                                                                    >  1:819353/1‑62 (MQ=25)                                                                                      aCCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAgcgcg                                                                                                          <  2:1059936/68‑1 (MQ=21)                                                                                      aCCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAgcgcg                                                                                                          >  1:1059936/1‑68 (MQ=21)                                                                                      aCCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                              >  2:1053429/1‑64 (MQ=18)                                                                                      aCCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                              <  1:1053429/64‑1 (MQ=18)                                                                                                gTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTg                               >  1:1052070/1‑133 (MQ=255)                                                                                                     ggCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCgtgg                                                                                        >  1:464682/1‑71 (MQ=14)                                                                                                     ggCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCgtgg                                                                                        <  2:464682/71‑1 (MQ=14)                                                                                                     ggCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTgg                         >  1:171197/1‑134 (MQ=255)                                                                                                      gCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTggg                        >  1:728771/1‑134 (MQ=255)                                                                                                           gcgcGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCaa                    >  1:1112635/1‑133 (MQ=255)                                                                                                                    gCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAgg          >  1:160741/1‑134 (MQ=255)                                                                                                                     cACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAg           <  2:171197/132‑1 (MQ=255)                                                                                                                     cACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAg           <  2:1052070/132‑1 (MQ=255)                                                                                                                     cACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGACCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAggg         >  1:451243/1‑134 (MQ=25)                                                                                                                         aGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAGGGCGcc     <  2:160741/134‑1 (MQ=255)                                                                                                                          ggAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAGGGCGCCg    <  1:242783/134‑1 (MQ=255)                                                                                                                              gCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAGGGCGCCGTc  >  1:48469/1‑132 (MQ=255)                                                                                                                              gCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAGGGCGCCGTc  >  2:635387/1‑132 (MQ=255)                                                                                                                                    |                                                                                                                              CGCGCCAGCGCTTCCAGCGCCGGTTGCACCACCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTT‑GCGGCAAA‑GTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAGGGCGCCGTC  >  AM260480/159250‑159504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑