Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	159,389	+G	intergenic (‑21/‑334)	h16_B0138 ← / → h16_B0139	transcriptional regulator, IclR‑family/conserved hypothetical transmembrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	159,388	1	.	G	100.0%	141.9 / NA	42	intergenic (‑20/‑335)	h16_B0138/h16_B0139	transcriptional regulator, IclR‑family/conserved hypothetical transmembrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (20/22);  total (20/22)

AGCGCCGGTTGCACCACCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTT‑GCGGCAAA‑GTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAGGGCGCCGTCCAGGGTCTTGAG  >  AM260480/159265‑159516                                                                                                                              |                                                                                                                                 aGCGCCGGTTGCACCACCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                                          <  2:845494/134‑1 (MQ=255) aGCGCCGGTTGCACCACCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                                          <  2:50042/134‑1 (MQ=255) aGCGCCGGTTGCACCACCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                                          <  1:1113540/134‑1 (MQ=255) aGCGCCGGTTGCACCACCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                                          <  2:443366/134‑1 (MQ=255) aGCGCCGGTTGCACCACCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                                          <  2:305049/134‑1 (MQ=255) aGCGCCGGTTGCACCACCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                                          <  1:684407/134‑1 (MQ=255)            caccacCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGc                                                                                                                <  2:170609/133‑1 (MQ=255)                accTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGcaccac                                                                                                           <  2:464518/134‑1 (MQ=255)                       ccggcGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCgtgg                                                                                                    <  1:1100465/134‑1 (MQ=255)                       ccggcGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCgtgg                                                                                                    <  1:1152471/134‑1 (MQ=255)                       ccggcGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCgtgg                                                                                                    <  1:822440/134‑1 (MQ=255)                       ccggcGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCgtgg                                                                                                    <  1:705048/134‑1 (MQ=255)                               aaCGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCAcgcc                                                                                            >  2:619803/1‑134 (MQ=255)                               aaCGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCAcgcc                                                                                            >  1:767066/1‑134 (MQ=255)                                                 tGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTc                                                                          >  2:242783/1‑134 (MQ=255)                                                      cAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGAAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCa                                                                     >  2:87209/1‑134 (MQ=25)                                                            gAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGcc                                                                                                                            >  1:1009701/1‑73 (MQ=25)                                                            gAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGcc                                                                                                                            <  2:1009701/73‑1 (MQ=25)                                                            gAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                                          <  1:789070/75‑1 (MQ=25)                                                            gAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                                          >  2:789070/1‑75 (MQ=25)                                                                 aGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCgtgg                                                                                                    <  1:605389/92‑1 (MQ=21)                                                                 aGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCgtgg                                                                                                    >  2:605389/1‑92 (MQ=21)                                                                 aGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAA‑gg                                                                                                                                >  1:819353/1‑62 (MQ=25)                                                                 aGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAA‑gg                                                                                                                                <  2:819353/63‑2 (MQ=25)                                                                       aCCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAgcgcg                                                                                                                      >  1:1059936/1‑68 (MQ=21)                                                                       aCCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAgcgcg                                                                                                                      <  2:1059936/68‑1 (MQ=21)                                                                       aCCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                                          >  2:1053429/1‑64 (MQ=18)                                                                       aCCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAg                                                                                                                          <  1:1053429/64‑1 (MQ=18)                                                                                 gTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTg                                           >  1:1052070/1‑133 (MQ=255)                                                                                      ggCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCgtgg                                                                                                    >  1:464682/1‑71 (MQ=14)                                                                                      ggCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCgtgg                                                                                                    <  2:464682/71‑1 (MQ=14)                                                                                      ggCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTgg                                     >  1:171197/1‑134 (MQ=255)                                                                                       gCCAAGCGCGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTggg                                    >  1:728771/1‑134 (MQ=255)                                                                                            gcgcGTCCTGCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCaa                                >  1:1112635/1‑133 (MQ=255)                                                                                                     gCACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAgg                      >  1:160741/1‑134 (MQ=255)                                                                                                      cACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAg                       <  2:171197/132‑1 (MQ=255)                                                                                                      cACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAg                       <  2:1052070/132‑1 (MQ=255)                                                                                                      cACCAGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGACCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAggg                     >  1:451243/1‑134 (MQ=25)                                                                                                          aGGAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAGGGCGcc                 <  2:160741/134‑1 (MQ=255)                                                                                                           ggAAGCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAGGGCGCCg                <  1:242783/134‑1 (MQ=255)                                                                                                               gCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAGGGCGCCGTc              >  2:635387/1‑132 (MQ=255)                                                                                                               gCCGTTGGCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAGGGCGCCGTc              >  1:48469/1‑132 (MQ=255)                                                                                                                  gttggCGGCAAAGGTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAGGGCGCCGTCCAggg         >  1:167087/5‑134 (MQ=255)                                                                                                                         gCAAAGGTCGCCAGCGCGCGGTGCACCACGCCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAGGGCGCCGTCCAGGGTCTTGAg  >  1:884201/1‑134 (MQ=255)                                                                                                                              |                                                                                                                                 AGCGCCGGTTGCACCACCTCGGCCGGCGAAAACGTCTTGCGCATCACTTGTCCCAGCTCGAACCAGCGCAACCCCAGGCCGTAGCGGCCAAGCGCGTCCTGCACCAGGAAGCCGTT‑GCGGCAAA‑GTCGCCAGCGCGCGGTGCACCACGGCGTGGTGCACGCCGCTGTAGCGCGCCAGTTCGCGCACGCCCCAGCTGGGCTGGCGGGCGGTGAAGTGGGTCAACAGCGCCAGGGCGCCGTCCAGGGTCTTGAG  >  AM260480/159265‑159516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑