Predicted mutation
evidence seq id position mutation annotation gene description
RA AM260480 2,135,859 +C intergenic (‑237/‑97) h16_B1889 ← / → h16_B1890 transcriptional regulator, IclR‑family/APC transporter, AAT family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM2604802,135,8571.C100.0% 110.2 / NA 29intergenic (‑235/‑99)h16_B1889/h16_B1890transcriptional regulator, IclR‑family/APC transporter, AAT family
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (12/17);  total (12/17)

AAAGAACAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTG‑CCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATGATCCAACAACCAGAGTCCGGCAACGGG  >  AM260480/2135724‑2135985
                                                                                                                                      |                                                                                                                                
aaaGAACAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                                   <  1:141309/134‑1 (MQ=255)
aaaGAACAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                                   >  2:141309/1‑134 (MQ=255)
aaaGAACAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                                   >  1:735374/1‑134 (MQ=255)
aaaGAACAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                                   <  1:528935/134‑1 (MQ=255)
 aaGAACAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg‑c                                                                                                                                 <  2:1076871/134‑1 (MQ=255)
  aGAACAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg‑cc                                                                                                                                >  1:468116/1‑134 (MQ=255)
     aCAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg‑ccctg                                                                                                                             <  2:909286/134‑4 (MQ=255)
       aaTTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                                   >  1:1158849/1‑127 (MQ=255)
       aaTTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                                   <  2:1158849/127‑1 (MQ=255)
           tttCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATc                                                                                                                         <  2:735374/133‑1 (MQ=255)
                               gTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg‑ccc                                                                                                                               >  1:801881/1‑105 (MQ=255)
                               gTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg‑ccc                                                                                                                               <  2:801881/106‑2 (MQ=255)
                                      aGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCgcctgc                                                                                             <  1:439931/134‑1 (MQ=255)
                                                 gagaCAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTg                                                                                  <  2:468116/134‑1 (MQ=255)
                                                   gaCAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTa                                                                                <  2:432302/134‑1 (MQ=255)
                                                            aCATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg‑ccctg                                                                                                                             <  2:1078513/79‑4 (MQ=255)
                                                            aCATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg‑ccctg                                                                                                                             >  1:1078513/1‑76 (MQ=255)
                                                             cATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTg                                                                             <  1:566331/127‑1 (MQ=255)
                                                             cATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTg                                                                             >  2:566331/1‑127 (MQ=255)
                                                                       tAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg‑ccctg                                                                                                                             >  2:329150/1‑65 (MQ=255)
                                                                       tAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg‑ccctg                                                                                                                             <  1:329150/68‑4 (MQ=255)
                                                                                   ccccGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCaa                                                                      >  2:344124/1‑112 (MQ=255)
                                                                                   ccccGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCaa                                                                      <  1:344124/112‑1 (MQ=255)
                                                                                   ccccGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCt                                                      <  1:295572/128‑1 (MQ=255)
                                                                                   ccccGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCt                                                      >  2:295572/1‑128 (MQ=255)
                                                                                   ccccGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAgcg                                                  >  1:208132/1‑132 (MQ=255)
                                                                                    cccGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAgcgc                                                 <  2:862510/132‑1 (MQ=255)
                                                                                      cGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCgcagca                                             <  1:822515/134‑1 (MQ=255)
                                                                                        ggATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTa                                                                                         <  1:884400/88‑1 (MQ=255)
                                                                                        ggATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTa                                                                                         >  2:884400/1‑88 (MQ=255)
                                                                                        ggATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCgcagca                                             >  1:238822/1‑132 (MQ=255)
                                                                                         gATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAg                                          <  1:616243/134‑1 (MQ=255)
                                                                                           ttGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGga                                        <  2:208132/134‑1 (MQ=255)
                                                                                                  tACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGt                                                                <  1:789787/103‑1 (MQ=255)
                                                                                                  tACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGt                                                                >  2:789787/1‑103 (MQ=255)
                                                                                                  tACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACacaa                                 <  1:1127143/134‑1 (MQ=255)
                                                                                                       aaCGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCt                                                                                                                        >  2:8561/1‑42 (MQ=255)
                                                                                                       aaCGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCt                                                                                                                        <  1:8561/42‑1 (MQ=255)
                                                                                                                   cccAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTg                                                                                  <  2:718746/68‑1 (MQ=255)
                                                                                                                   cccAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTg                                                                                  >  1:718746/1‑68 (MQ=255)
                                                                                                                   cccAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAgcgc                                                 >  1:888494/1‑101 (MQ=255)
                                                                                                                   accAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAgcgc                                                 <  2:888494/100‑1 (MQ=255)
                                                                                                                          tGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATGATCCAACAACCAGAGTCCgg         >  1:426094/1‑134 (MQ=255)
                                                                                                                                 tACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATGATCCAACAACCAGAGTCCGGCAACggg  >  2:555739/1‑134 (MQ=255)
                                                                                                                                      |                                                                                                                                
AAAGAACAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTG‑CCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATGATCCAACAACCAGAGTCCGGCAACGGG  >  AM260480/2135724‑2135985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑