Predicted mutation
evidence seq id position mutation annotation gene description
RA AM260479 581,821 +C coding (117/1734 nt) h16_A0555 → zinc metalloprotease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM260479581,8201.C100.0% 94.1 / NA 25S39T (AGC→ACC) h16_A0555zinc metalloprotease
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (15/10);  total (15/10)

GGCGCCGGCCATTGCCGCGCTGGCGGCGCATGGACGCCATGGCGGCGGCCGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAG‑CACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCGTGAAGGCGGGCCAGAAGTCCGAGTTC  >  AM260479/581694‑581947
                                                                                                                               |                                                                                                                               
ggCGCCGGCCATTGCCGCGCTGGCGGCGCATGGACGCCATGGCGGCGGCCGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGcc                                                                                                                           >  1:159959/1‑134 (MQ=255)
               cgcgCTGGCGGCGCATGGACGCCATGGCGGCGGCCGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGcc                                                                                                            >  2:1120439/1‑134 (MQ=255)
                     ggcggcGCATGGACGCCATGGCGGCGGCCGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGc                                                                                                      >  1:521364/1‑134 (MQ=255)
                      gcggcgCATGGACGCCATGGCGGCGGCCGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGc                                                                                                      <  2:159959/133‑1 (MQ=255)
                                gACGCCATGGCGGCGGCCGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCg                                                                                            >  2:187535/1‑133 (MQ=255)
                                                 cGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGc                                                                                                      <  2:764099/106‑1 (MQ=255)
                                                 cGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGc                                                                                                      >  1:764099/1‑106 (MQ=255)
                                                   cTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCAtctc                                                                        >  2:704444/1‑134 (MQ=255)
                                                                  gcgcTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGc                                                           <  1:1120439/132‑1 (MQ=255)
                                                                                      ggCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGa                                     <  2:521364/134‑1 (MQ=255)
                                                                                      ggCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGa                                     <  1:187535/134‑1 (MQ=255)
                                                                                                cAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGcc                                                                             >  1:826566/1‑84 (MQ=255)
                                                                                                cAGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGcc                                                                             <  2:826566/84‑1 (MQ=255)
                                                                                                 aGGGTACGCCGCGCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCGt                           >  2:522790/1‑133 (MQ=255)
                                                                                                          cgcgCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGa                                     <  1:318976/114‑1 (MQ=255)
                                                                                                          cgcgCACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGa                                     >  2:318976/1‑114 (MQ=255)
                                                                                                           gcgcACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGc                                                                                        >  2:1023042/1‑62 (MQ=255)
                                                                                                           gcgcACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGc                                                                                        <  1:1023042/62‑1 (MQ=255)
                                                                                                           gcgcACGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCGTGAAGGCGGGc                 >  2:588467/1‑133 (MQ=255)
                                                                                                           gcgcACGGCACCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCGTGAAGGCGGGc                 >  2:866057/1‑133 (MQ=255)
                                                                                                                cGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAg                                                                 >  1:998590/1‑80 (MQ=255)
                                                                                                                cGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAg                                                                 <  2:998590/80‑1 (MQ=255)
                                                                                                                cGGCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCGTGAAGGCGGGCCAGAAg           >  2:237703/1‑134 (MQ=255)
                                                                                                                  gCCCCGGCGCCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCGTGAAGGCGGGCCAGAAGTc         >  2:540253/1‑134 (MQ=255)
                                                                                                                         cgcCAGCCACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCGTGAAGGCGGGCCAGAAGTCCGAGTTc  <  1:704444/134‑1 (MQ=255)
                                                                                                                               |                                                                                                                               
GGCGCCGGCCATTGCCGCGCTGGCGGCGCATGGACGCCATGGCGGCGGCCGCTGGCGGCGCTGCTGGCGCTGACGTTCGCGGCGCCGGCCTGGCCCCAGGGTACGCCGCGCACGGCCCCGGCGCCAG‑CACGCCGGTGGCGGCTGCGCCCGCGGCCAGGCCCCCGCAGCCGGTGCCCGCCATCTCGGGCGAGGCCAGCGACCAGGTCTATGACAACCTGAACCGCAGCGTGAAGGCGGGCCAGAAGTCCGAGTTC  >  AM260479/581694‑581947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑