Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	735,524	+G	intergenic (‑409/+175)	h16_A0685 ← / ← h16_A0686	hypothetical membrane spanning protein/Predictedpermease/CBS‑domain‑containing membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	735,521	1	.	G	100.0%	121.1 / NA	32	intergenic (‑406/+178)	h16_A0685/h16_A0686	hypothetical membrane spanning protein/Predictedpermease/CBS‑domain‑containing membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (17/15);  total (17/15)

ACAAAAAGTAACCAAAAAACGCGTCGCCTGAGCGGCCGGCAATCAATTCCAGGGTGTTGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCA‑GGGCAGTGCCTGCGTTAACCCACTATTGAACGATCCCCATGCAGGGCGCAGGCCGCCTGCTGCGAGCGGCATCGCGGGGACGCCTACGGCTGCTGCGCGGGCTCAAAGTGCATCGTCGGTTTCTTCTG  >  AM260479/735393‑735649                                                                                                                                  |                                                                                                                                 aCAAAAAGTAACCAAAAAACGCGTCGCCTGAGCGGCCGGCAATCAATTCCAGGGTGTTGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCA‑gggg                                                                                                                              >  2:675882/1‑132 (MQ=255) aCAAAAAGTAACCAAAAAACGCGTCGCCTGAGCGGCCGGCAATCAATTCCAGGGTGTTGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCA‑gggg                                                                                                                              >  2:1034927/1‑132 (MQ=255)  cAAAAAGTAACCAAAAAACGCGTCGCCTGAGCGGCCGGCAATCAATTCCAGGGTGTTGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCA‑ggggca                                                                                                                            <  2:430395/134‑4 (MQ=255)   aaaaaGTAACCAAAAAACGCGTCGCCTGAGCGGCCGGCAATCAATTCCAGGGTGTTGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCA‑ggggcag                                                                                                                           <  1:1034927/134‑5 (MQ=255)   aaaaaGTAACCAAAAAACGCGTCGCCTGAGCGGCCGGCAATCAATTCCAGGGTGTTGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCA‑ggggcag                                                                                                                           <  2:225800/134‑5 (MQ=255)   aaaaaGTAACCAAAAAACGCGTCGCCTGAGCGGCCGGCAATCAATTCCAGGGTGTTGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCA‑ggggcag                                                                                                                           <  1:675882/134‑5 (MQ=255)        gTAACCAAAAAACGCGTCGCCTGAGCGGCCGGCAATCAATTCCAGGGTGTTGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCt                                                                                                                       <  2:719742/134‑1 (MQ=255)             cAAAAAACGCGTCGCCTGAGCGGCCGGCAATCAATTCCAGGGTGTTGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGtt                                                                                                                  <  1:633326/134‑1 (MQ=255)              aaaaaaCGCGTCGCCTGAGCGGCCGGCAATCAATTCCAGGGTGTTGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGtt                                                                                                                  >  2:633326/1‑133 (MQ=255)                    cgcgTCGCCTGAGCGGCCGGCAATCAATTCCAGGGTGTTGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGTTAACCCAc                                                                                                           >  2:1124379/1‑134 (MQ=255)                          gCCTGAGCGGCCGGCAATCAATTCCAGGGTGTTGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGTTAACCCACTATTg                                                                                                      >  2:629772/1‑133 (MQ=255)                          gCCTGAGCGGCCGGCAATCAATTCCAGGGTGTTGGTGGTTCGCACGGTGGTGCTTGCCGTGCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGTTAACCCACTATTg                                                                                                      <  2:512105/133‑1 (MQ=255)                                       gcAATCAATTCCAGGGTGTTGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGtt                                                                                                                  >  2:744098/1‑108 (MQ=255)                                       gcAATCAATTCCAGGGTGTTGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGtt                                                                                                                  <  1:744098/108‑1 (MQ=255)                                          atcaatTCCAGGGTGTTGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGTTAACCCACTATTGAACGATCCCCATGCAgg                                                                                     <  1:1124379/134‑1 (MQ=255)                                                         ttGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGc                                                                                                                     <  1:290320/87‑1 (MQ=255)                                                         ttGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGc                                                                                                                     >  2:290320/1‑87 (MQ=255)                                                         ttGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGTTAACCCACTAtt                                                                                                       <  1:565209/101‑1 (MQ=255)                                                         ttGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGTTAACCCACTAtt                                                                                                       >  2:565209/1‑101 (MQ=255)                                                         ttGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGTTAACCCACTATTGAACGATCCCCATGCAGGGCGCAGGCCGCctgc                                                                      >  2:584639/1‑134 (MQ=255)                                                         ttGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCA‑ggggcag                                                                                                                           >  2:876796/1‑76 (MQ=255)                                                         ttGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCA‑ggggcag                                                                                                                           <  1:876796/80‑5 (MQ=255)                                                                     aCGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGTTAACCCACTATTGAACGATCCCCATGCAGGGCGCAGGCCGCCTGCTGCGAGCGGCAt                                                          >  2:233842/1‑134 (MQ=255)                                                                       ggtggtGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGTTaa                                                                                                                >  2:640195/1‑78 (MQ=255)                                                                       ggtggtGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGTTaa                                                                                                                <  1:640195/78‑1 (MQ=255)                                                                        gtggtgCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGTTAACCCACTATTGAACGATCCCCATGCAGGGCGCAGGCCGCCTGCTGCGAGCGGCATcgc                                                       <  1:584639/134‑1 (MQ=255)                                                                             gCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCt                                                                                                                       >  1:160247/1‑65 (MQ=255)                                                                             gCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCt                                                                                                                       <  2:160247/65‑1 (MQ=255)                                                                                     ttCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGtt                                                                                                                  <  1:110375/62‑1 (MQ=255)                                                                                     ttCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGtt                                                                                                                  >  2:110375/1‑62 (MQ=255)                                                                                       ccGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGTTaa                                                                                                                >  1:1137408/1‑62 (MQ=255)                                                                                       ccGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGTTaa                                                                                                                <  2:1137408/62‑1 (MQ=255)                                                                                       ccGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGTTAACCCACTATTGAACg                                                                                                  <  1:881843/76‑1 (MQ=255)                                                                                       ccGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGTTAACCCACTATTGAACg                                                                                                  >  2:881843/1‑76 (MQ=255)                                                                                          gCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGTTAACCCACTATTGAACGATCCCCATGCAGGGCGCAGGCCGCCTGCTGCGAGCGGCATCGCGGGGACGCCTACGgctgc                                     <  2:894982/134‑1 (MQ=255)                                                                                                   cccGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGTTAACCCACTATTGAACGATCCCCATGCAGGGCGCAGGCCGCCTGCTGCGAGCGGCATCGCGGGGACGCCTACGGCTGCTGCGCGGGc                            >  1:1003053/1‑134 (MQ=255)                                                                                                   cccGTTCGACCCTGCTACGCCATGTGAGTCAGGGGCAGTGCCTGCGTTAACCCACTATTGAACGATCCCCATGCAGGGCGCAGGCCGCCTGCTGCGAGCGGCATCGCGGGGACGCCTACGGCTGCTGCGCGGGc                            >  1:122390/1‑134 (MQ=255)                                                                                                                             aGTCAGGGGCAGTGCCTGCGTTAACCCACTATTGAACGATCCCCATGCAGGGCGCAGGCCGCCTGCTGCGAGCGGCATCGCGGGGACGCCTACGGctgctg                                   >  1:1085193/1‑101 (MQ=255)                                                                                                                             aGTCAGGGGCAGTGCCTGCGTTAACCCACTATTGAACGATCCCCATGCAGGGCGCAGGCCGCCTGCTGCGAGCGGCATCGCGGGGACGCCTACGGctgctg                                   <  2:1085193/101‑1 (MQ=255)                                                                                                                             aGTCAGGGGCAGTGCCTGCGTTAACCCACTATTGAACGATCCCCATGCAGGGCGCAGGCCGCCTGCTGCGAGCGGCATCGCGGGGACGCCTACGGCTGCTGCGCGGGCTCAAAGTGCATCGTCGGTTTCTTCTg  >  2:944361/1‑134 (MQ=255)                                                                                                                                  |                                                                                                                                 ACAAAAAGTAACCAAAAAACGCGTCGCCTGAGCGGCCGGCAATCAATTCCAGGGTGTTGGTGGTTCGCACGGTGGTGCTTGCCGTTCCGGCGTGGTTGCCCGTTCGACCCTGCTACGCCATGTGAGTCA‑GGGCAGTGCCTGCGTTAACCCACTATTGAACGATCCCCATGCAGGGCGCAGGCCGCCTGCTGCGAGCGGCATCGCGGGGACGCCTACGGCTGCTGCGCGGGCTCAAAGTGCATCGTCGGTTTCTTCTG  >  AM260479/735393‑735649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑