Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	782,330	+G	intergenic (‑680/‑42)	h16_A0723 ← / → h16_A0724	hypothetical membrane protein/hypothetical membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	782,330	1	.	G	100.0%	121.7 / NA	33	intergenic (‑680/‑42)	h16_A0723/h16_A0724	hypothetical membrane protein/hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (18/15);  total (18/15)

GGCCTTGGTTCTGCTTGTACATCGGTGGGCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGC‑ATG‑GCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGCCGTGCTGATCATCTATGGTGCCTTGATCGTGG  >  AM260479/782202‑782453                                                                                                                                  |                                                                                                                             ggCCTTGGTTCTGCTTGTACATCGGTGGGCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGcgat                                                                                                                            >  2:919462/1‑129 (MQ=255)          tCTGCTTGTACATCGGTGGGCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGAc                                                                                                                  >  1:139087/1‑133 (MQ=255)           cTGCTTGTACATCGGTGGGCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCa                                                                                                                >  1:1132960/1‑134 (MQ=255)           cTGCTTGTACATCGGTGGGCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCa                                                                                                                >  2:383359/1‑134 (MQ=255)                            ggCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGc                                                                                               <  1:75260/134‑1 (MQ=255)                                              aGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTcgc                                                                             <  1:450319/134‑1 (MQ=255)                                                   gTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCAt                                                                        <  2:83305/134‑1 (MQ=255)                                                       aTCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGAt                                                                                  <  2:651262/120‑1 (MQ=255)                                                       aTCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGAt                                                                                  >  1:651262/1‑120 (MQ=255)                                                       aTCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCAt                                                                        >  2:497567/1‑130 (MQ=255)                                                       aTCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCAt                                                                        <  1:497567/130‑1 (MQ=255)                                                       aTCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGc                                                                    >  1:393239/1‑134 (MQ=255)                                                            tGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGAc                                                               >  2:408912/1‑134 (MQ=255)                                                            tGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGAc                                                               >  1:723838/1‑134 (MQ=255)                                                             gCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCg                                                                                             <  2:323854/103‑1 (MQ=255)                                                             gCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCg                                                                                             >  1:323854/1‑103 (MQ=255)                                                             gCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGAcc                                                              >  2:385694/1‑134 (MQ=255)                                                                  gccgcTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAg                                                         >  2:1124060/1‑134 (MQ=255)                                                                     gcTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGt                                                      <  1:919462/134‑1 (MQ=255)                                                                                   gaagaaTCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAg                                        >  2:131052/5‑134 (MQ=255)                                                                                    agaaATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGc                                       <  2:420629/134‑1 (MQ=255)                                                                                    agaaATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGc                                       <  1:383359/134‑1 (MQ=255)                                                                                         tCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGc                                  <  1:385694/134‑1 (MQ=255)                                                                                                    cTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTg                                                                      >  2:226760/1‑87 (MQ=255)                                                                                                    cTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTg                                                                      <  1:226760/87‑1 (MQ=255)                                                                                                                 tCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGCCGTGCTGATCATCTATGGTGCCt           <  1:1124060/133‑1 (MQ=255)                                                                                                                     ggggATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCggg                                               >  1:966474/1‑93 (MQ=255)                                                                                                                     ggggATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCggg                                               <  2:966474/93‑1 (MQ=255)                                                                                                                      gggATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGa                                                                                   <  2:977358/56‑1 (MQ=255)                                                                                                                      gggATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGa                                                                                   >  1:977358/1‑56 (MQ=255)                                                                                                                        gATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGCCGTGCTGATCATCTATGGTGCCTTGATCGTg   >  2:834514/1‑134 (MQ=255)                                                                                                                         aTGGGGAGCGATGAGCCTGGACCATAGCCCGgcagca                                                                                                   <  2:470818/37‑1 (MQ=21)                                                                                                                         aTGGGGAGCGATGAGCCTGGACCATAGCCCGgcagca                                                                                                   >  1:470818/1‑37 (MQ=21)                                                                                                                         aTGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGCCGTGCTGATCATCTATGGTGCCTTGATCGTgg  >  1:173460/1‑134 (MQ=255)                                                                                                                                  |                                                                                                                             GGCCTTGGTTCTGCTTGTACATCGGTGGGCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGC‑ATG‑GCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGCCGTGCTGATCATCTATGGTGCCTTGATCGTGG  >  AM260479/782202‑782453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑