Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	782,333	+A	intergenic (‑683/‑39)	h16_A0723 ← / → h16_A0724	hypothetical membrane protein/hypothetical membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	782,333	1	.	A	100.0%	120.4 / NA	33	intergenic (‑683/‑39)	h16_A0723/h16_A0724	hypothetical membrane protein/hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (18/15);  total (18/15)

TCTGCTTGTACATCGGTGGGCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGC‑ATG‑GCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGCCGTGCTGATCATCTATGGTGCCTTGATCGTGG  >  AM260479/782211‑782453                                                                                                                             |                                                                                                                         tCTGCTTGTACATCGGTGGGCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGAc                                                                                                                  >  1:139087/1‑133 (MQ=255)  cTGCTTGTACATCGGTGGGCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCa                                                                                                                >  1:1132960/1‑134 (MQ=255)  cTGCTTGTACATCGGTGGGCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCa                                                                                                                >  2:383359/1‑134 (MQ=255)                   ggCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGc                                                                                               <  1:75260/134‑1 (MQ=255)                                     aGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTcgc                                                                             <  1:450319/134‑1 (MQ=255)                                          gTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCAt                                                                        <  2:83305/134‑1 (MQ=255)                                              aTCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGAt                                                                                  <  2:651262/120‑1 (MQ=255)                                              aTCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGAt                                                                                  >  1:651262/1‑120 (MQ=255)                                              aTCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCAt                                                                        >  2:497567/1‑130 (MQ=255)                                              aTCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCAt                                                                        <  1:497567/130‑1 (MQ=255)                                              aTCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGc                                                                    >  1:393239/1‑134 (MQ=255)                                                   tGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGAc                                                               >  1:723838/1‑134 (MQ=255)                                                   tGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGAc                                                               >  2:408912/1‑134 (MQ=255)                                                    gCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCg                                                                                             >  1:323854/1‑103 (MQ=255)                                                    gCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCg                                                                                             <  2:323854/103‑1 (MQ=255)                                                    gCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGAcc                                                              >  2:385694/1‑134 (MQ=255)                                                         gccgcTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAg                                                         >  2:1124060/1‑134 (MQ=255)                                                            gcTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGt                                                      <  1:919462/134‑1 (MQ=255)                                                                          gaagaaTCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAg                                        >  2:131052/5‑134 (MQ=255)                                                                           agaaATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGc                                       <  1:383359/134‑1 (MQ=255)                                                                           agaaATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGc                                       <  2:420629/134‑1 (MQ=255)                                                                                tCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGc                                  <  1:385694/134‑1 (MQ=255)                                                                                           cTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTg                                                                      >  2:226760/1‑87 (MQ=255)                                                                                           cTGCCACAGAATATCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTg                                                                      <  1:226760/87‑1 (MQ=255)                                                                                                        tCAGGGGGATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGCCGTGCTGATCATCTATGGTGCCt           <  1:1124060/133‑1 (MQ=255)                                                                                                            ggggATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCggg                                               <  2:966474/93‑1 (MQ=255)                                                                                                            ggggATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCggg                                               >  1:966474/1‑93 (MQ=255)                                                                                                             gggATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGa                                                                                   >  1:977358/1‑56 (MQ=255)                                                                                                             gggATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGa                                                                                   <  2:977358/56‑1 (MQ=255)                                                                                                               gATGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGCCGTGCTGATCATCTATGGTGCCTTGATCGTg   >  2:834514/1‑134 (MQ=255)                                                                                                                aTGGGGAGCGATGAGCCTGGACCATAGCCCGgcagca                                                                                                   <  2:470818/37‑1 (MQ=21)                                                                                                                aTGGGGAGCGATGAGCCTGGACCATAGCCCGgcagca                                                                                                   >  1:470818/1‑37 (MQ=21)                                                                                                                aTGGGGAGCGATGAGCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGCCGTGCTGATCATCTATGGTGCCTTGATCGTgg  >  1:173460/1‑134 (MQ=255)                                                                                                                             |                                                                                                                         TCTGCTTGTACATCGGTGGGCGCCTGACCAGAATGGAGCCAGTACATCGATGCATCGCCGCTGCATTGCCAAGAAGAAATCAGGCCGGCGCTGCCACAGAATATCAGGGGGATGGGGAGC‑ATG‑GCCTGGACCATAGCCCGGCAGCAACGCCGGGGTCAGTGATGTCGCGGCATTGGCTGGACCTGGAGCGTCTCCGGGCCTACAGCGTTGCCGTGCTGATCATCTATGGTGCCTTGATCGTGG  >  AM260479/782211‑782453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑