Predicted mutation
evidence seq id position mutation annotation gene description
RA AM260479 275,969 +C coding (268/558 nt) h16_A0264 ← Hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM260479275,9681.C100.0% 145.2 / NA 38A90G (GCC→GGC) h16_A0264Hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (16/22);  total (16/22)

CCGCCGGGTGGAGTTGCGCAAGCGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGG‑CTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAGCGCGGTGCGATCCTCGCCATCGA  >  AM260479/275837‑276095
                                                                                                                                    |                                                                                                                               
ccgccgGGTGGAGTTGCGCAAGCGGCTTGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGG‑cc                                                                                                                               >  2:392333/1‑133 (MQ=255)
     gggTGGAGTTGCGCAAGCGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTgcgc                                                                                                                           >  2:754643/1‑134 (MQ=255)
      ggTGGAGTTGCGCAAGCGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTgcgcg                                                                                                                          <  1:344263/134‑1 (MQ=255)
            gTTGCGCAAGCGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGAtt                                                                                                                    <  1:1021529/134‑1 (MQ=255)
            gTTGCGCAAGCGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGAtt                                                                                                                    <  1:40634/134‑1 (MQ=255)
            gTTGCGCAAGCGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGAtt                                                                                                                    <  1:179735/134‑1 (MQ=255)
                  cAAGCGGCTTGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTgg                                                                                                              <  1:392333/134‑1 (MQ=255)
                    aGCGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGAt                                                                                                            <  1:525112/134‑1 (MQ=255)
                      cGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCg                                                                                                          <  2:567572/134‑1 (MQ=255)
                      cGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCg                                                                                                          <  1:754643/134‑1 (MQ=255)
                                                           aGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCggcggc                                                                     <  1:130794/134‑1 (MQ=255)
                                                            gggCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGcggcc                                                                    >  1:36850/1‑134 (MQ=255)
                                                                           tGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCcagcagcag                                                     >  1:168887/1‑134 (MQ=255)
                                                                              aaTAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCcagcagcagcag                                                  <  1:1035563/134‑1 (MQ=255)
                                                                              aaTAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCcagcagcagcag                                                  <  2:407739/134‑1 (MQ=255)
                                                                                      tCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTgc                                          >  2:1080189/1‑134 (MQ=255)
                                                                                       ccTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGAtt                                                                                                                    <  1:1127577/59‑1 (MQ=255)
                                                                                       ccTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGAtt                                                                                                                    >  2:1127577/1‑59 (MQ=255)
                                                                                       ccTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCgtgg                                             <  1:1131426/130‑1 (MQ=255)
                                                                                       ccTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCgtgg                                             >  2:1131426/1‑130 (MQ=255)
                                                                                         ttGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCg                                                                                                          <  2:76975/67‑1 (MQ=255)
                                                                                         ttGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCg                                                                                                          >  1:76975/1‑67 (MQ=255)
                                                                                           gCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAgg                                     >  2:1154656/1‑134 (MQ=255)
                                                                                            ccTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAgg                                     <  1:1154656/133‑1 (MQ=255)
                                                                                               tCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCAc                                 >  2:184084/1‑134 (MQ=255)
                                                                                                    gCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGaa                            >  1:840016/1‑134 (MQ=255)
                                                                                                           tAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAAt                           <  2:1039622/128‑1 (MQ=255)
                                                                                                           tAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAAt                           >  1:1039622/1‑128 (MQ=255)
                                                                                                           tAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAgcgc                      <  1:1080189/133‑1 (MQ=255)
                                                                                                                    ccGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAAt                           <  2:1150234/119‑1 (MQ=255)
                                                                                                                    ccGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAAt                           >  1:1150234/1‑119 (MQ=255)
                                                                                                                      ggTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCg                                                                                                          >  2:36528/1‑38 (MQ=37)
                                                                                                                      ggTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCg                                                                                                          <  1:36528/38‑1 (MQ=37)
                                                                                                                      ggTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAGCGCGGTGCGATCCTc          >  2:573603/1‑134 (MQ=255)
                                                                                                                         cGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAGCGCgg                    >  2:1060530/1‑121 (MQ=255)
                                                                                                                         cGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAGCGCgg                    <  1:1060530/121‑1 (MQ=255)
                                                                                                                         cGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAGCGCGGTGCGATCCTCGc        >  2:307192/1‑133 (MQ=255)
                                                                                                                          gTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAGCGCGGTGCGATCCTCGCCa      <  1:573603/134‑1 (MQ=255)
                                                                                                                              cGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAGCGCGGTGCGATCCTCGCCATCGa  <  2:168887/134‑1 (MQ=255)
                                                                                                                                    |                                                                                                                               
CCGCCGGGTGGAGTTGCGCAAGCGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGG‑CTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAGCGCGGTGCGATCCTCGCCATCGA  >  AM260479/275837‑276095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑