| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260479 | 696,526 | 0 | T | A | 23.8% | 40.0 / 11.2 | 23 | intergenic (+4/‑125) | h16_A0653/h16_A0654 | conserved hypothetical protein/Hemolysin‑coregulated protein |
| Reads supporting (aligned to +/- strand): ref base T (7/9); new base A (2/3); total (10/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGAGGAACTGGAAGGCAACCCCGGCTACTACAGCGCCAAGTTCTTCCTGCGTCCGCACTTCCAGCTCGAGGGCCTGACCGTTCCGCTGCGGCTTGTGGCGCGCCTGCCGTCGGTCAAGGACGCCGCCTGAGCGTCGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATCGCGCCCCTGACCTGATTGGGGCGCGGCCCTGGCTGTGGCTCTTGTCTGCAGTACCTGTCTTAACGTCAAGGAGTTTTGCAATGG > AM260479/696393‑696654 | cgAGGAACTGGAAGGCAACCCCGGCTACTACAGCGCCAAGTTCTTCCTGCGTCCGCACTTCCAGCTCGAGGGCCTGACCGTTCCGCTGCGGCTTGTGGCGCGCCTGCCGTCGGTCAAGGACGCCGCCTGAGCGt > 2:354844/1‑134 (MQ=255) gAGGAACTGGAAGGCAACCCCGGCTACTACAGCGCCAAGTTCTTCCTGCGTCCGCACTTCCAGCTCGAGGGCCTGACCGTTCCGCTGCGGCTTGTGGCGCGCCTGCCGTCGGTCAAGGACGCCGCCTGAGCGt < 1:310070/133‑1 (MQ=255) aGGCAACCCCGGCTACTACAGCGCCAAGTTCTTCCTGCGTCCGCACTTCCAGCTCGAGGGCCTGACCGTTCCGCTGCGGCTTGTGGCGCGCCTGCCGTCGGTCAAGGACGCCGCCTGAGCGACGCTCAGGCGtt < 2:730540/134‑1 (MQ=255) ccGGCTACTACAGCGCCAAGTTCTTCCTGCGTCCGCACTTCCAGCTCGAGGGCCTGACCGTTCCGCTGCGGCTTGTGGCGCGCCTGCCGTCGGTCAAGGACGCCGCCTGAGCGTCGCTCAGGCGTTGAAACGTa < 1:354844/134‑1 (MQ=255) aaGTTCTTCCTGCGTCCGCACTTCCAGCTCGAGGGCCTGACCGTTCCGCTGCGGCTTGTTGCGCGCCTGCCGTCGGTCAAGGACGCCGCCTGAGCGTCGCTCAGGCGTTGAAACGTACCGGTAGt > 1:242568/1‑125 (MQ=255) aaGTTCTTCCTGCGTCCGCACTTCCAGCTCGAGGGCCTGACCGTTCCGCTGCGGCTTGTTGCGCGCCTGCCGTCGGTCAAGGACGCCGCCTGAGCGTCGCTCAGGCGTTGAAACGTACCGGTAGt < 2:242568/125‑1 (MQ=255) gTTCTTCCTGCGTCCGCACTTCCAGCTCGAGGGCCTGACCGTTCCGCTGCGGCTTGTGGCGCGCCTGCCGTCGGTCAAGGACGCCGCCTGAGCGACGCTCAGGCg > 1:245083/1‑105 (MQ=255) gTTCTTCCTGCGTCCGCACTTCCAGCTCGAGGGCCTGACCGTTCCGCTGCGGCTTGTGGCGCGCCTGCCGTCGGTCAAGGACGCCGCCTGAGCGACGCTCAGGCg < 2:245083/105‑1 (MQ=255) cTGCGGCTTGTGGCGCGCCTGCCGTCGGTCAAGGACGCCGCCTGAGCGTCGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATCGCGCCCCTGACCTGATTGGGGCGCGGCCCTGGCTGTGGctc > 1:273771/1‑134 (MQ=255) tgGCGCGCCTGCCGTCGGTCAAGGACGCCGCCTGAGCGTCGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATCGCGCCCCTGACCTGATTGGGGCGCGGCCCTGGCTGTGGCTCTTGTCTGCa < 1:678167/133‑1 (MQ=255) tgGCGCGCCTGCCGTCGGTCAAGGACGCCGCCTGAGCGTCGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATCGCGCCCCTGACCTGATTGGGGCGCGGCCCTGGCTGTGGCTCTTGTCTGCa < 2:273771/133‑1 (MQ=255) gcgcgcCTGCCGTCGGTCAAGGACGCCGCCTGAGCGTCGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATCGCGCCCCTGACCTGATTGGGGCGCGGCCCTGGCt > 1:864696/1‑115 (MQ=255) gcgcgcCTGCCGTCGGTCAAGGACGCCGCCTGAGCGTCGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATCGCGCCCCTGACCTGATTGGGGCGCGGCCCTGGCt < 2:864696/115‑1 (MQ=255) aaGGACGCCGCCTGAGCGTCGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATCGCGCCCCTGACCTGATTgg > 2:410807/1‑82 (MQ=255) aaGGACGCCGCCTGAGCGTCGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATCGCGCCCCTGACCTGATTgg < 1:410807/82‑1 (MQ=255) aaGGACGCCGCCTGAGCGTCGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATCGCGCCCCTGACCTGATTGGGGCGCGGCCCTGGCTGTGGCTCTTGTCTGCAGTACCTGTCTTAACGTCAAg > 2:378869/1‑133 (MQ=255) aaGGACGCCGCCTGAGCGTCGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATCGCGCCCCTGACCTGATTGGGGCGCGGCCCTGGCTGTGGCTCTTGTCTGCAGTACCTGTCTTAACGTCAAg > 2:826028/1‑133 (MQ=255) aCGCCGCCTGAGCGTCGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATCGCGCCCCTGACCTGATTGGGGCGCGGCCCTGGCTGTGGCTCTTGTCTGCAGTACCTGTCTTAACGTCAAGGAGtt < 1:701605/134‑1 (MQ=255) cgccgcCTGAGCGCCGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATCGCGCCCCTGACCTGATTGGGGCGCGGCCCTGGCTGTGGct > 2:1085027/1‑98 (MQ=255) cgccgcCTGAGCGCCGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATCGCGCCCCTGACCTGATTGGGGCGCGGCCCTGGCTGTGGct < 1:1085027/98‑1 (MQ=255) tGAGCGTCGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATcgc > 2:1161186/1‑53 (MQ=255) tGAGCGTCGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATcgc < 1:1161186/53‑1 (MQ=255) tGAGCGACGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATcgc < 1:461027/53‑1 (MQ=255) tGAGCGACGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATcgc > 2:461027/1‑53 (MQ=255) aGCGTCGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATCGCGCCCCTGACCTGATTGGGGCGCGGCCCTGGCTGTGGCTCTTGTCTGCAGTACCTGTCTTAACGTCAAGGAGTTTTGCAATgg < 1:826028/133‑1 (MQ=255) | CGAGGAACTGGAAGGCAACCCCGGCTACTACAGCGCCAAGTTCTTCCTGCGTCCGCACTTCCAGCTCGAGGGCCTGACCGTTCCGCTGCGGCTTGTGGCGCGCCTGCCGTCGGTCAAGGACGCCGCCTGAGCGTCGCTCAGGCGTTGAAACGTACCGGTAGTTCCACGCCGAGGTATCGCGCCCCTGACCTGATTGGGGCGCGGCCCTGGCTGTGGCTCTTGTCTGCAGTACCTGTCTTAACGTCAAGGAGTTTTGCAATGG > AM260479/696393‑696654 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |