| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260479 | 2,137,055 | 0 | T | A | 30.0% | 30.8 / 14.4 | 20 | L241Q (CTG→CAG) | catB3 | muconate cyclo‑isomerase |
| Reads supporting (aligned to +/- strand): ref base T (8/6); new base A (3/3); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.62e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAACCAGGCCTGGAACGAAGCGGATGCCGCCACCGGCATTGCCATGCTGGAGACCGCCGGCATCGACCTGATCGAGCAGCCCACGCCACGCGAGCAACGCGCCGCGCTGGCGCGCCTG‑GCGGCGCGTTTCGTGGTGCCCATCATGGCCGACGAAGCGGTGTGCGGGCCCGAGGACGCGATGGAACTGGCCCGCATCGGCGGCGCCGACGTGTTCGCCCTGAAGATCGCCAAGTCGGGC > AM260479/2136948‑2137185 | cAACCAGGCCTGGAACGAAGCGGATGCCGCCACCGGCATTGCCATGCTGGAGACCGCCGGCATCGACCTGATCGAGCAGCCCACGCCACGCGAGCAACGCGCCGCGCTGGCGCGCCTG‑GCGGCGCGt > 2:954736/1‑127 (MQ=255)cAACCAGGCCTGGAACGAAGCGGATGCCGCCACCGGCATTGCCATGCTGGAGACCGCCGGCATCGACCTGATCGAGCAGCCCACGCCACGCGAGCAACGCGCCGCGCTGGCGCGCCTG‑GCGGCGCGt < 1:954736/127‑1 (MQ=255) ggATGCCGCCACCGGCATTGCCATGCTGGAGACCGCCGGCATCGACCTGATCGAGCAGCCCACGCCACGCGAGCAACGCGCCGCGCTGGCGCGCCTG‑GCGGCGCGTTTCGTGGTGCCcat < 2:945116/120‑1 (MQ=255) ggATGCCGCCACCGGCATTGCCATGCTGGAGACCGCCGGCATCGACCTGATCGAGCAGCCCACGCCACGCGAGCAACGCGCCGCGCTGGCGCGCCTG‑GCGGCGCGTTTCGTGGTGCCcat > 1:945116/1‑120 (MQ=255) ggATGCCGCCACCGGCATTGCCATGCTGGAGACCGCCGGCATCGACCTGATCGAGCAGCCCACGCCACGCGAGCAACGCGCCGCGCTGGCGCGCCTG‑GCGGCGCGTTTCGTGGTGCCCATCATGGCcgacga < 2:825333/132‑1 (MQ=255) ggATGCCGCCACCGGCATTGCCATGCTGGAGACCGCCGGCATCGACCTGATCGAGCAGCCCACGCCACGCGAGCAACGCGCCGCGCTGGCGCGCCTG‑GCGGCGCGTTTCGTGGTGCCCATCATGGCcgacga > 1:825333/1‑132 (MQ=255) ggATGCCGCCACCGGCATTGCCATGCTGGAGACCGCCGGCATCGACCTGATCGAGCAGCCCACGCCACGCGAGCAACGCGCCGCGCTGGCGCGCCTG‑GCGGCGCGTTTCGTGGTGCCCATCATGGCCGACGAAg > 2:105366/1‑134 (MQ=255) ggATGCCGCCACCGGCATTGCCATGCTGGAGACCGCCGGCATCGACCTGATCGAGCAGCCCACGCCACGCGAGCAACGCGCCGC‑CAGGCGCGCCAGCGCGGCGCGTTTCGTGGTGCCCATCATGGCcgac > 2:433124/1‑130 (MQ=37) ggATGCCGCCACCGGCATTGCCATGCTGGAGACCGCCGGCATCGACCTGATCGAGCAGCCCACGCCACGCGAGCAACGCGCCGC‑CAGGCGCGCCAGCGCGGCGCGTTTCGTGGTGCCCATCATGGCcgac < 1:433124/130‑1 (MQ=37) aTTGCCATGCTGGAGACCGCCGGCATCGACCTGATCGAGCAGCCCACGCCACGCGAGCAACGCGCCGC‑CAGGCGCGccagcg < 1:550714/82‑5 (MQ=25) aTTGCCATGCTGGAGACCGCCGGCATCGACCTGATCGAGCAGCCCACGCCACGCGAGCAACGCGCCGC‑CAGGCGCGccagcg > 2:550714/1‑78 (MQ=25) gccgGCATCGACCTGATCGAGCAGCCCACGCCACGCGAGCAACGCGCCGCGCTGGCGCGCCTG‑GCGGCGCGTTTCGTGGTGCCCATCATGGCcg > 1:599974/1‑94 (MQ=255) gccgGCATCGACCTGATCGAGCAGCCCACGCCACGCGAGCAACGCGCCGCGCTGGCGCGCCTG‑GCGGCGCGTTTCGTGGTGCCCATCATGGCcg < 2:599974/94‑1 (MQ=255) cATCGACCTGATCGAGCAGCCCACGCCACGCGAGCAACGCGCCGC‑CAGGCGCGCCAGCGCGGCGCGt < 2:250605/67‑1 (MQ=18) cATCGACCTGATCGAGCAGCCCACGCCACGCGAGCAACGCGCCGC‑CAGGCGCGCCAGCGCGGCGCGt > 1:250605/1‑67 (MQ=18) aTCGAGCAGCCCACGCCACGCGAGCAACGCGCCGCGCTGGCGCGCCTG‑GCGGCGCGTTTCGTGGTGCCCATCATGGCCGACGAAGCGGTGTGCGGGCCCGAGGACGCGATGGAACTGGCCCGCATCGgcggcg < 1:105366/133‑1 (MQ=255) aaaCGCGCCGCGCTGGCGCGCCTG‑GCGGCGCGTTTCGTGGTGCCCATCATGGCCGACGAAGCGGTGTGCTGGCCCGAGGACGCGATGGAACt < 1:183698/91‑1 (MQ=255) aaaCGCGCCGCGCTGGCGCGCCTG‑GCGGCGCGTTTCGTGGTGCCCATCATGGCCGACGAAGCGGTGTGCGGGCCCGAGGACGCGATGGAACt > 2:183698/2‑92 (MQ=255) cgccgcgcTGGCGCGCCTG‑GCGGCGCGTTTCGTGGTGCCCATCATGGCCGACGAAGCGGTGTGCGGGCCCGAGGACGCGATGGAACTGGCCCGCATCGGCGGCGCCGACGTGTTCGCCCTGAAGATCGCCAAGt > 1:708192/1‑134 (MQ=255) cgcTGGCGCGCCTG‑GCGGCGCGTTTCGTGGTGCCCATCATGGCCGACGAAGCGGTGTGCGGGCCCGAGGACGCGATGGAACTGGCCCGCATCGGCGGCGCCGACGTGTTCGCCCTGAAGATCGCCAAGTCGggc > 2:458267/1‑134 (MQ=255) | CAACCAGGCCTGGAACGAAGCGGATGCCGCCACCGGCATTGCCATGCTGGAGACCGCCGGCATCGACCTGATCGAGCAGCCCACGCCACGCGAGCAACGCGCCGCGCTGGCGCGCCTG‑GCGGCGCGTTTCGTGGTGCCCATCATGGCCGACGAAGCGGTGTGCGGGCCCGAGGACGCGATGGAACTGGCCCGCATCGGCGGCGCCGACGTGTTCGCCCTGAAGATCGCCAAGTCGGGC > AM260479/2136948‑2137185 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |