Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM2604792,244,3170CA23.5% 80.5 / 18.4 34G326G (GGG→GGT) smcchromosome segregation ATPase
Reads supporting (aligned to +/- strand):  ref base C (12/14);  new base A (4/4);  total (16/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCAGTTCGTCGTTCTTGTGGGCGACGGCTTCCTGCGCCTCGACCGTGCGGCCTTCGGCCACGGCCAGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGCTTGCTAACTTCGGCGTTGGCCTCATACAGCGCGCCCTGCGCGGCGTGCATGCCATCGGA  >  AM260479/2244184‑2244448
                                                                                                                                     |                                                                                                                                   
gCAGTTCGTCGTTCTTGTGGGCGACGGCTTCCTGCGCCTCGACCGTGCGGCCTTCGGCCACGGCCAGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGc                                                                                                                                     >  2:792677/1‑134 (MQ=255)
 cAGTTCGTCGTTCTTGTGGGCGACGGCTTCCTGCGCCTCGACCGTGCGGCCTTCGGCCACGGCCAGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGcc                                                                                                                                    <  1:179673/134‑1 (MQ=255)
                   ggCGACGGCTTCCTGCGCCTCGACCGTGCGGCCTTCGGCCACGGCCAGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCt                                                                                                                  <  2:305376/134‑1 (MQ=255)
                       aCGGCTTCCTGCGCCTCGACCGTGCGGCCTTCGGCCACGGCCAGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAgcg                                                                                                                           <  2:47256/121‑1 (MQ=255)
                       aCGGCTTCCTGCGCCTCGACCGTGCGGCCTTCGGCCACGGCCAGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAgcg                                                                                                                           >  1:47256/1‑121 (MQ=255)
                       aCGGCTTCCTGCGCCTCGACCGTGCGGCCTTCGGCCACGGCCAGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGc                                                                                                              >  2:929873/1‑134 (MQ=255)
                        cGGCTTCCTGCGCCTCGACCGTGCGGCCTTCGGCCACGGCCAGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGcc                                                                                                             <  2:239572/134‑1 (MQ=255)
                                                ggCCTTCTGCCACGGCCAGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGACCGGGCAGCGCGGCGATCTGGGCCTGCAcgcg                                                                                                     <  1:198158/118‑1 (MQ=255)
                                                ggCCTTCTGCCACGGCCAGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGACCGGGCAGCGCGGCGATCTGGGCCTGCAcgcg                                                                                                     >  2:198158/1‑118 (MQ=255)
                                                ggCCTTCGGCCACGGCCAGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTccac                                                                                      >  1:547167/1‑133 (MQ=255)
                                                ggCCTTCGGCCACGGCCAGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTccac                                                                                      >  2:333594/1‑133 (MQ=255)
                                                ggCCTTCGGCCACGGCCAGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGACCGGGCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTccac                                                                                      >  2:686094/1‑133 (MQ=255)
                                                 gCCTTCGGCCACGGCCAGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGAt                                                                                                   <  1:611057/119‑1 (MQ=255)
                                                 gCCTTCGGCCACGGCCAGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGAt                                                                                                   >  2:611057/1‑119 (MQ=255)
                                                 gCCTTCGGCCACGGCCAGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTccacca                                                                                    >  1:428285/1‑134 (MQ=255)
                                                         ccacggccaGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCgg                                                                            <  2:833123/134‑1 (MQ=255)
                                                           acggccaGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGAt                                                                          >  2:742024/1‑134 (MQ=255)
                                                                       tcttcGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAg                                                              >  1:323005/1‑134 (MQ=255)
                                                                           cGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGc                                                             <  1:742024/131‑1 (MQ=255)
                                                                                 gcgcCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGCTTGCTAACt                                                    >  2:974974/1‑134 (MQ=255)
                                                                                    ccAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGCTTGCTAACTTCg                                                 <  1:974974/134‑1 (MQ=255)
                                                                                    ccAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGCTTGCTAACTTCg                                                 <  2:819493/134‑1 (MQ=255)
                                                                                    ccAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGCTTGCTAACTTCg                                                 <  2:489478/134‑1 (MQ=255)
                                                                                    ccAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGCTTGCTAACTTCg                                                 <  2:428285/134‑1 (MQ=255)
                                                                                    ccAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGCTTGCTAACTTCg                                                 <  2:323005/134‑1 (MQ=255)
                                                                                      aGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGCTTGCTAACTTCGGc                                               >  1:284803/1‑134 (MQ=255)
                                                                                                  gCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGACCGGGCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGCTTGCTAACTTCGGCGTTGGCCTCAt                                    <  1:686094/133‑1 (MQ=255)
                                                                                                           gCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGCTTGCTAACTTCGGCGTTGGCCTCATACAGCGCGcc                          >  1:547086/1‑134 (MQ=255)
                                                                                                           gCCTTGCCCTGCCACTGCTCGCGCTGACCGGGCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCtt                                                                  <  2:217823/94‑1 (MQ=255)
                                                                                                           gCCTTGCCCTGCCACTGCTCGCGCTGACCGGGCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCtt                                                                  >  1:217823/1‑94 (MQ=255)
                                                                                                            ccTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGCTTGCTAACTTCGGCGTTGGCCTCATACAGCGCGccc                         <  2:547086/134‑1 (MQ=255)
                                                                                                                gCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGCTTGCTAACTTCGGCGTTGGCCTCATACAGCGCGCCCTg                       <  2:284803/132‑1 (MQ=255)
                                                                                                                     gCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGCTTGCTAACTTCGGCGTTGGCCTCATACAGCGCGCCCTGCgcggcg                >  1:322024/1‑134 (MQ=255)
                                                                                                                       cACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGCTTGCTAACTTCGGCGTTGGCCTCATACAGCGCGCCCTGCGCGGCGTg              <  1:792677/134‑1 (MQ=255)
                                                                                                                               gcgcTGACCGGGCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGCTTGCTAACTTCg                                                 <  2:822306/91‑1 (MQ=255)
                                                                                                                               gcgcTGACCGGGCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGCTTGCTAACTTCg                                                 >  1:822306/1‑91 (MQ=255)
                                                                                                                                     cccGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGCTTGCTAACTTCGGCGTTGGCCTCATACAGCGCGCCCTGCGCGGCGTGCATGCCATCGGa  <  2:322024/132‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                   
GCAGTTCGTCGTTCTTGTGGGCGACGGCTTCCTGCGCCTCGACCGTGCGGCCTTCGGCCACGGCCAGGTTTTCTTCGGCCTGCGCCAGCTCGTCGGTGGCCTGTTCGGCCTTGCCCTGCCACTGCTCGCGCTGCCCGGTCAGCGCGGCGATCTGGGCCTGCACGCGATTGCGCGATTCCACCACGTAGCGGATCTCGGCTTCGAGCTTGCTAACTTCGGCGTTGGCCTCATACAGCGCGCCCTGCGCGGCGTGCATGCCATCGGA  >  AM260479/2244184‑2244448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑