Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	3,720,402	G→T	D170Y (GAC→TAC)	h16_A3441 →	periplasmic multimodular transpeptidase/transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	3,720,402	0	G	T	100.0%	97.8 / NA	26	D170Y (GAC→TAC)	h16_A3441	periplasmic multimodular transpeptidase/transglycosylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/8);  total (18/8)

CTACACGCGCAAGATCTACGAGATGCTGCTGGCGTACAAGATCGAGGCCAACCTGAACAAGGACCAGATCCTCGAGCTGTACATGAACCAGATCTATCTGGGGCAGCGCGCCGACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACGCCGGCCGAGGCCGCCATGCTGGCCGGCCTGCCCAAGGCGCCGTCGGCGTACAACCCGGTGGTCAACCCGC  >  AM260479/3720290‑3720528                                                                                                                 |                                                                                                                               cTACACGCGCAAGATCTACGAGATGCTGCTGGCGTACAAGATCGAGGCCAACCTGAACAAGGACCAGATCCTCGAGCTGTACATGAACCAGATCTATCTGGGGCAGCGCGCCTACGGCTTCGACAGCGCcgcgc                                                                                                           >  1:359565/1‑134 (MQ=255)             gATCTACGAGATGCTGCTGGCGTACAAGATCGAGGCCAACCTGAACAAGGACCAGATCCTCGAGCTGTACATGAACCAGATCTATCTGGGGCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCg                                                                                               >  2:758212/1‑134 (MQ=255)                               ggCGTACAAGATCGAGGCCAACCTGAACAAGGACCAGATCCTCGAGCTGTACATGAACCAGATCTATCTGGGGCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACg                                                                             >  2:521646/1‑134 (MQ=255)                                 cGTACAAGATCGAGGCCAACCTGAACAAGGACCAGATCCTCGAGCTGTACATGAACCAGATCTATCTGGGGCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGt                                                                            >  1:396624/1‑133 (MQ=255)                                         aTCGAGGCCAACCTGAACAAGGACCAGATCCTCGAGCTGTACATGAACCAGATCTATCTGGGGCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACgccggc                                                                   >  1:840199/1‑134 (MQ=255)                                              ggCCAACCTGAACAAGGACCAGATCCTCGAGCTGTACATGAACCAGATCTATCTGGGGCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCgg                                                                                      <  2:379045/110‑1 (MQ=255)                                              ggCCAACCTGAACAAGGACCAGATCCTCGAGCTGTACATGAACCAGATCTATCTGGGGCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCgg                                                                                      >  1:379045/1‑110 (MQ=255)                                                 cAACCTGAACAAGGACCAGATCCTCGAGCTGTACATGAACCAGATCTATCTGGGGCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCgg                                                                                      <  1:1111768/107‑1 (MQ=255)                                                 cAACCTGAACAAGGACCAGATCCTCGAGCTGTACATGAACCAGATCTATCTGGGGCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCgg                                                                                      >  2:1111768/1‑107 (MQ=255)                                                                 cAGATCCTCGAGCTGTACATGAACCAGATCTATCTGGGGCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACGCCGGCCGAGGCCGCCATGCTGGCCGGCCt                                           >  2:1022963/1‑134 (MQ=255)                                                                           aGCTGTACATGAACCAGATCTATCTGGGGCAGAGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACGCCGGCCGAGGCCGCCATGCTGGCCGGCCTGCCCAAGgcg                                 <  1:791384/134‑1 (MQ=255)                                                                            gCTGTACATGAACCAGATCTATCTGGGGCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACGCCGGCCGAGGCCGCCATGCTGGCCGGCCTGCCCAAGgcgc                                <  1:1022963/134‑1 (MQ=255)                                                                                    tGAACCAGATCTATCTGGGGCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACGCCGGCCGAGGCCGCCATGCTGGCCGGCCTGCCCAAGGCGCCGTCGGCg                        >  2:753860/1‑134 (MQ=255)                                                                                              ctatctGGGGCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACGCCGGCCGAGGCCGCCATGCTGGCCGGCCTGCCCAAGGCGCCGTCGGc                         >  1:639493/1‑123 (MQ=255)                                                                                              ctatctGGGGCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACGCCGGCCGAGGCCGCCATGCTGGCCGGCCTGCCCAAGGCGCCGTCGGc                         <  2:639493/123‑1 (MQ=255)                                                                                              ctatctGGGGCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACGCCGGCCGAGGCCGCCATGCTGGCCGGCCTGCCCAAGGCGCCGTCGGCGTACAACCCgg              >  1:258518/1‑134 (MQ=255)                                                                                              ctatctGGGGCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACGCCGGCCGAGGCCGCCATGCTGGCCGGCCTGCCCAAGGCGCCGTCGGCGTACAACCCgg              >  2:900384/1‑134 (MQ=255)                                                                                              ctatctGGGGCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACGCCGGCCGAGGCCGCCATGCTGGCCGGCCTGCCCAAGGCGCCGTCGGCGTACAACCCgg              >  2:197885/1‑134 (MQ=255)                                                                                                atctGGGGCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACgccgg                                                                    >  2:160295/1‑78 (MQ=255)                                                                                                atctGGGGCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACgccgg                                                                    <  1:160295/78‑1 (MQ=255)                                                                                                     gggCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACGCCGGCCGAGGCCGCCATGCTGGCCGGCCTGCCCAAGGCGCCGTCGGCGTACAACCCGGTGGTCaa       >  1:155671/1‑134 (MQ=255)                                                                                                       gCAGCGCGCCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACGCCGGCCGAGGCCGCCATGCTGGCCGGCCTGCCCAAGGCGCCGTCGGCGTACAACCCGGTGGTCAAcc     <  2:155671/134‑1 (MQ=255)                                                                                                          gcgcgcCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACGCCGGCCGAGGCCGCCATGCTGGCCGGCCTGCCCAAGgcg                                 >  2:322287/1‑103 (MQ=255)                                                                                                          gcgcgcCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACGCCGGCCGAGGCCGCCATGCTGGCCGGCCTGCCCAAGgcg                                 <  1:322287/103‑1 (MQ=255)                                                                                                          gcgcgcCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACGCCGGCCGAGGCCGCCATGCTGGCCGGCCTGCCCAAGGCGCCGTCGGCGTACAACCCGGTGGTCAACccgc  >  1:243056/1‑134 (MQ=255)                                                                                                          gcgcgcCTACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACGCCGGCCGAGGCCGCCATGCTGGCCGGCCTGCCCAAGGCGCCGTCGGCGTACAACCCGGTGGTCAACccgc  >  1:247385/1‑134 (MQ=255)                                                                                                                 |                                                                                                                               CTACACGCGCAAGATCTACGAGATGCTGCTGGCGTACAAGATCGAGGCCAACCTGAACAAGGACCAGATCCTCGAGCTGTACATGAACCAGATCTATCTGGGGCAGCGCGCCGACGGCTTCGACAGCGCCGCGCGCGTGTATTTCGGCAAGTCGGTGCGCGACGTGACGCCGGCCGAGGCCGCCATGCTGGCCGGCCTGCCCAAGGCGCCGTCGGCGTACAACCCGGTGGTCAACCCGC  >  AM260479/3720290‑3720528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑