Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	3,905,524	G→C	A1285G (GCC→GGC)	putA ←	bifunctional proline dehydrogenase and proline oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	3,905,524	0	G	C	100.0%	150.6 / NA	40	A1285G (GCC→GGC)	putA	bifunctional proline dehydrogenase and proline oxidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (18/22);  total (18/22)

GAGGTCCGAGGGTTGCCTCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGGCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCATCGATAGCGATGTCCGCCGCGGTCCAGTCGGCCACCAT  >  AM260479/3905395‑3905651                                                                                                                                  |                                                                                                                                gAGGTCCGAGGGTTGCCTCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTg                                                                                                                             >  1:104714/1‑134 (MQ=255)    gTCCGAGGGTTGCCTCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCAc                                                                                                                          <  1:537207/134‑1 (MQ=255)             ttGCCTCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCgatgat                                                                                                                 >  1:929454/1‑134 (MQ=255)                  tCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGAc                                                                                                            >  1:160717/1‑134 (MQ=255)                             tcatcaAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCAcc                                                                                                 >  1:1132626/1‑134 (MQ=255)                             tcatcaAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCAcc                                                                                                 <  2:160717/134‑1 (MQ=255)                              catcaAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCg                                                                                                >  1:350450/1‑134 (MQ=255)                               atcaAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGc                                                                                               <  1:1081210/134‑1 (MQ=255)                               atcaAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGc                                                                                               <  2:715442/134‑1 (MQ=255)                                  aaaCTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCa                                                                                             <  1:425818/133‑1 (MQ=255)                                            tGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGgc                                                                                                      >  1:233786/1‑114 (MQ=255)                                            tGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGgc                                                                                                      <  2:233786/114‑1 (MQ=255)                                            tGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGc                                                                                               <  1:915472/121‑1 (MQ=255)                                            tGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGc                                                                                               >  2:915472/1‑121 (MQ=255)                                            tGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGca                                                                                   >  1:715478/1‑133 (MQ=255)                                            tGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGca                                                                                   >  2:403774/1‑133 (MQ=255)                                                   cgcgGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTgc                                                                           <  2:715478/134‑1 (MQ=255)                                                                   aCCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAAtc                                                           <  2:593758/134‑1 (MQ=255)                                                                   aCCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAAtc                                                           <  2:104714/134‑1 (MQ=255)                                                                    ccGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAAtc                                                           >  1:336083/1‑133 (MQ=255)                                                                    ccGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAAtc                                                           <  2:336083/133‑1 (MQ=255)                                                                            gagcgCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAAtc                                                           >  1:436423/1‑125 (MQ=255)                                                                            gagcgCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAAtc                                                           <  2:436423/125‑1 (MQ=255)                                                                                      tCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCa                                        <  1:1029215/134‑1 (MQ=255)                                                                                        aGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTgca                                                >  1:477375/1‑124 (MQ=255)                                                                                        aGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTgca                                                <  2:477375/124‑1 (MQ=255)                                                                                                    aGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGc                                                                      >  2:817457/1‑90 (MQ=255)                                                                                                    aGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGc                                                                      <  1:817457/90‑1 (MQ=255)                                                                                                    aGCGCGATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCATCGATAGCGATGTc                          >  1:7128/1‑134 (MQ=255)                                                                                                       gcgATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCAcc                                           <  1:903244/114‑1 (MQ=255)                                                                                                       gcgATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCAcc                                           >  2:903244/1‑114 (MQ=255)                                                                                                        cgATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCg                                                                                     <  2:396423/71‑1 (MQ=255)                                                                                                        cgATATTCGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCg                                                                                     >  1:396423/1‑71 (MQ=255)                                                                                                               cGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAAtc                                                           >  2:587296/1‑90 (MQ=255)                                                                                                               cGGTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAAtc                                                           <  1:587296/90‑1 (MQ=255)                                                                                                                 gTTCACCTTGCGCCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCATCGATAGCGATGTCCGCCGCGGTCCAg             <  2:863699/134‑1 (MQ=255)                                                                                                                           cgcCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCATCGATAGCGATGTCCGCCGCGGTCCAGTCGGccacca   <  1:403774/134‑1 (MQ=255)                                                                                                                            gcCAGGCCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCATCGATAGCGATGTCCGCCGCGGTCCAGTCGGCCACCAt  <  2:929454/134‑1 (MQ=255)                                                                                                                               aGGCCCTGCACGCCGATGATCGGACCGGGGCGCAcc                                                                                                 >  2:704928/1‑36 (MQ=255)                                                                                                                               aGGCCCTGCACGCCGATGATCGGACCGGGGCGCAcc                                                                                                 <  1:704928/36‑1 (MQ=255)                                                                                                                                  |                                                                                                                                GAGGTCCGAGGGTTGCCTCAGCCGATCGTCATCAAACTGGCATTGCCGCCCGCGGCTGCGGTGTTGACCGAAAGCGAGCGCTCGATCAGCAAACGGTCGAGCGCGATATTCGGTTCACCTTGCGCCAGGGCCTGCACGCCGATGATCGGACCGGGGCGCACCGCCACCTGCTCGCACACCGTGCGCAGCTGGTCGGAATCTCCGTGGTGCAGCACCGCATCGATAGCGATGTCCGCCGCGGTCCAGTCGGCCACCAT  >  AM260479/3905395‑3905651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑