Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	3,983,831	C→T	D23N (GAC→AAC)	ntrX ←	response regulator containing a Fis‑type DNA‑binding domain, NtrX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	3,983,831	0	C	T	100.0%	177.1 / NA	46	D23N (GAC→AAC)	ntrX	response regulator containing a Fis‑type DNA‑binding domain, NtrX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/22);  total (24/22)

AGGGTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTCGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGACG  >  AM260479/3983710‑3983939                                                                                                                          |                                                                                                             aGGGTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGAtctc                                                                                                  >  2:186854/1‑134 (MQ=255)   ggTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCg                                                                                                <  2:567541/134‑1 (MQ=255)       aCGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGagag                                                                                            >  1:331484/1‑134 (MQ=255)       aCGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGagag                                                                                            >  1:828732/1‑134 (MQ=255)       aCGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGacag                                                                                            >  2:1101243/1‑134 (MQ=255)                   tCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGAt                                                                                >  2:591001/1‑134 (MQ=255)                       gCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTcc                                                                             >  2:1151163/1‑133 (MQ=255)                            cAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCAttt                                                                        <  2:235904/133‑1 (MQ=255)                            cAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTc                                                                       <  2:835782/134‑1 (MQ=255)                                         gcaCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTc                                                                    <  1:423094/124‑1 (MQ=255)                                         gcaCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTc                                                                    >  2:423094/1‑124 (MQ=255)                                         gcaCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTAc                                                           >  2:914096/1‑133 (MQ=255)                                         gcaCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTAc                                                           >  2:1020975/1‑133 (MQ=255)                                         gcaCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTAc                                                           >  1:771686/1‑133 (MQ=255)                                         gcaCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTAc                                                           >  2:423959/1‑133 (MQ=255)                                          caCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTTGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGa                                                                                 <  2:390674/110‑1 (MQ=255)                                          caCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGa                                                                                 >  1:390674/1‑110 (MQ=255)                                             gAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGa                                                      <  1:591001/134‑1 (MQ=255)                                              aGGTCTGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGAt                                                     >  1:304630/1‑134 (MQ=255)                                              aGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTcc                                                                             <  2:550282/110‑1 (MQ=255)                                              aGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTcc                                                                             >  1:550282/1‑110 (MQ=255)                                                gTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCg                                                   <  2:771686/134‑1 (MQ=255)                                                   gggCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGtt                                                 <  1:914096/133‑1 (MQ=255)                                                        tACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATa                                           <  2:331484/134‑1 (MQ=255)                                                        tACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATa                                           <  1:423959/134‑1 (MQ=255)                                                          cccGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACt                                         <  1:1151163/134‑1 (MQ=255)                                                               cgcgcgGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTcccc                                    <  2:828732/134‑1 (MQ=255)                                                                       aTTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTccc                                     >  2:1076167/1‑125 (MQ=255)                                                                       aTTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTccc                                     <  1:1076167/125‑1 (MQ=255)                                                                                    cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAgg                                                       <  1:604630/94‑1 (MQ=255)                                                                                    cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAgg                                                       >  2:604630/1‑94 (MQ=255)                                                                                    cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATa                                           <  1:656292/106‑1 (MQ=255)                                                                                    cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATa                                           >  2:656292/1‑106 (MQ=255)                                                                                    cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCg                      >  2:33379/1‑127 (MQ=255)                                                                                    cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCg                      <  1:33379/127‑1 (MQ=255)                                                                                    cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGtt               >  2:728223/1‑134 (MQ=255)                                                                                    cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGtt               >  1:439501/1‑134 (MQ=255)                                                                                      gcgcGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTcc             >  2:225928/1‑134 (MQ=255)                                                                                               ccGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCa                                                                   <  2:544228/71‑1 (MQ=255)                                                                                               ccGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCa                                                                   >  1:544228/1‑71 (MQ=255)                                                                                                cgcgAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCgg     <  1:728223/132‑1 (MQ=255)                                                                                                 gcgAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGACg  <  2:304630/134‑1 (MQ=255)                                                                                                     gTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTc                                                                    <  2:661340/64‑1 (MQ=255)                                                                                                     gTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTc                                                                    >  1:661340/1‑64 (MQ=255)                                                                                                                    cTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGa                                                         <  1:925703/60‑1 (MQ=255)                                                                                                                    cTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGa                                                         >  2:925703/1‑60 (MQ=255)                                                                                                                          |                                                                                                             AGGGTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTCGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGACG  >  AM260479/3983710‑3983939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑