Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	164,760	C→T	A15V (GCC→GTC)	acoR →	transcriptional regulator of acetoin catabolism,PspF‑family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	164,760	0	C	T	100.0%	87.6 / NA	24	A15V (GCC→GTC)	acoR	transcriptional regulator of acetoin catabolism,PspF‑family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/11);  total (13/11)

TTCGCACGATCCACCGCCCCATCCGAAACATGGGGCCGACGGCAGCGACGACAGGAGACAGCGATGGACCTGCGGCAACGCGAGCACATCGAGACGGTGGTCCAGGCCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCATCATCCAGAACTCATGGCGCCGCTGCGTGCACCAGTACGGCCTGGATCCGTCGCGCATGCAGGAGGCGCGCA  >  AM260480/164654‑164888                                                                                                           |                                                                                                                                 ttCGCACGATCCACCGCCCCATCCGAAACATGGGGCCGACGGCAGCGACGACAGGAGACAGCGATGGACCTGCGGCAACGCGAGCACATCGAGACGGTGGTCCAGGTCACCACATACCTTGCCCCGCCGGCCGt                                                                                                       >  2:1021400/1‑134 (MQ=255)            cACCGCCCCATCCGAAACATGGGGCCGACGGCAGCGACGACAGGAGACAGCGATGGACCTGCGGCAACGCGAGCACATCGAGACGGTGGTCCAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGAcc                                                                                            <  2:572592/134‑1 (MQ=255)                gCCCCATCCGAAACATGGGGCCGACGGCAGCGACGACAGGAGACAGCGATGGACCTGCGGCAACGCGAGCACATCGAGACGGTGGTCCAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGAt                                                                                        <  1:1021400/134‑1 (MQ=255)                        cGAAACATGGGGCCGACGGCAGCGACGACAGGAGACAGCGATGGACCTGCGGCAACGCGAGCACATCGAGACGGTGGTCCAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCac                                                                                 >  1:215535/1‑133 (MQ=255)                                 gggCCGACGGCAGCGACGACAGGAGACAGCGATGGACCTGCGGCAACGCGAGCACATCGAGACGGTGGTCCAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCcatc                                                                        <  1:843603/133‑1 (MQ=255)                                    ccGACGGCAGCGACGACAGGAGACAGCGATGGACCTGCGGCAACGCGAGCACATCGAGACGGTGGTCCAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCatcatc                                                                     >  1:433217/1‑133 (MQ=255)                                        cGGCAGCGACGACAGGAGACAGCGATGGACCTGCGGCAACGCGAGCACATCGAGACGGTGGTCCAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCacgac                                                                              >  1:439292/1‑120 (MQ=255)                                        cGGCAGCGACGACAGGAGACAGCGATGGACCTGCGGCAACGCGAGCACATCGAGACGGTGGTCCAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCacgac                                                                              <  2:439292/120‑1 (MQ=255)                                                  gacAGGAGACAGCGATGGACCTGCGGCAACGCGAGCACATCGAGACGGTGGTCCAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCATCATCCAGAACTCATGgcgc                                                      >  1:404712/1‑134 (MQ=255)                                                     aGGAGACAGCGATGGACCTGCGGCAACGCGAGCACATCGAGACGGTGGTCCAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCATCATCCAGAACTCATGGcgccgc                                                   <  2:215535/134‑1 (MQ=255)                                                        agaCAGCGATGGACCTGCGGCAACGCGAGCACATCGAGACGGTGGTCCAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCATCATCCAGAACTCATGGCGCCGCTGc                                                <  2:497334/134‑1 (MQ=255)                                                                 tGGACCTGCGGCAACGCGAGCACATCGAGACGGTGGTCCAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCATCATCCAGAACTCATGGCGCCGCTGCGTGCACCAg                                       >  1:644940/1‑134 (MQ=255)                                                                  ggACCTGCGGCAACGCGAGCACATCGAGACGGTGGTCCAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCATCATCCAGAACTCATGGCGCCGCTGCGTGCAcc                                         >  1:63375/1‑131 (MQ=255)                                                                  ggACCTGCGGCAACGCGAGCACATCGAGACGGTGGTCCAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCATCATCCAGAACTCATGGCGCCGCTGCGTGCAcc                                         <  2:63375/131‑1 (MQ=255)                                                                       tGCGGCAACGCGAGCACATCGAGACGGTGGTCCAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCATCATCCAGAACTCATGGCGCCGCTGCGTGCACCAGTACGGc                                 >  1:630040/1‑134 (MQ=255)                                                                                   aGCACATCGAGACGGTGGTCCAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCATCATCCAGAACTCATGGCGCCGCTGCGTGCACCAGTACGGCCTGGATCCGTcg                     >  1:895326/1‑134 (MQ=255)                                                                                            agaCGGTGGTCCAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCATCATCCAGAACTCATGGCGCCGCTGCGTGCACCAGTACGGCCTGGATCCGTCGCGCATGCag            <  2:644940/134‑1 (MQ=255)                                                                                                      ccAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCATCATCCAGAACTCATGGCGCCGCTGCGTGCAcc                                         <  2:520293/95‑1 (MQ=255)                                                                                                      ccAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCATCATCCAGAACTCATGGCGCCGCTGCGTGCAcc                                         >  1:1071784/1‑95 (MQ=255)                                                                                                      ccAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCATCATCCAGAACTCATGGCGCCGCTGCGTGCAcc                                         <  2:1071784/95‑1 (MQ=255)                                                                                                      ccAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCATCATCCAGAACTCATGGCGCCGCTGCGTGCAcc                                         >  1:520293/1‑95 (MQ=255)                                                                                                      ccAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCATCATCCAGAACTCATGGCGCCGCTGCGTGCACCAGTACGGCCTGGATCCGTCGCGCATGCAGGAGgcgcgc   >  2:533748/1‑133 (MQ=255)                                                                                                      ccAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCATCATCCAGAACTCATGGCGCCGCTGCGTGCACCAGTACGGCCTGGATCCGTCGCGCATGCAGGAGgcgcgc   <  1:533748/133‑1 (MQ=255)                                                                                                      ccAGGTCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCATCATCCAGAACTCATGGCGCCGCTGCGTGCACCAGTACGGCCTGGATCCGTCGCGCATGCAGGAGGCGCGCa  >  1:881377/1‑134 (MQ=255)                                                                                                           |                                                                                                                                 TTCGCACGATCCACCGCCCCATCCGAAACATGGGGCCGACGGCAGCGACGACAGGAGACAGCGATGGACCTGCGGCAACGCGAGCACATCGAGACGGTGGTCCAGGCCACCACATACCTTGCCCCGCCGGCCGTGCTTGCGGACCGGATCGCGCACGACGCCATCATCCAGAACTCATGGCGCCGCTGCGTGCACCAGTACGGCCTGGATCCGTCGCGCATGCAGGAGGCGCGCA  >  AM260480/164654‑164888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑