Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	293,407	T→A	Y759N (TAC→AAC)	flhF →	Flagellar GTP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	293,407	0	T	A	100.0%	193.3 / NA	51	Y759N (TAC→AAC)	flhF	Flagellar GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (27/24);  total (27/24)

GGCCGCGCCCGCCTTCCTCGCGCGCCTGGCTGGCCGCACGGTGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAATACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCACGACGCGCCGTATCGCCGTG  >  AM260480/293286‑293537                                                                                                                          |                                                                                                                                   ggCCGCGCCCGCCTTCCTCGCGCGCCTGGCTGGCCGCACGGTGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGcc                                                                                                                        <  1:912238/134‑1 (MQ=255)                    gcgcgcCTGGCTGGCCGCACGGTGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCg                                                                                                      <  1:965867/133‑1 (MQ=255)                           tggctggcCGCACGGTGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGc                                                                                               >  2:1141917/1‑133 (MQ=255)                             gctggcCGCACGGTGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCg                                                                                              <  2:942804/132‑1 (MQ=255)                             gctggcCGCACGGTGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGAt                                                                                            <  1:1141917/134‑1 (MQ=255)                                          tGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAg                                                                               <  1:519352/134‑1 (MQ=255)                                             gcCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGt                                                                              >  1:834977/1‑132 (MQ=255)                                               cAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgc                                                                          <  2:867855/134‑1 (MQ=255)                                               cAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgc                                                                          <  2:834977/134‑1 (MQ=255)                                               cAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgc                                                                          <  2:1102509/134‑1 (MQ=255)                                                        ccggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGc                                                                  >  1:1158987/1‑133 (MQ=255)                                                        ccggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGc                                                                  >  1:18904/1‑133 (MQ=255)                                                          ggtgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCAt                                                               >  2:1063787/1‑134 (MQ=255)                                                            tgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCg                                                                                                      >  1:100989/1‑93 (MQ=255)                                                            tgccggGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCg                                                                                                      <  2:100989/93‑1 (MQ=255)                                                                     cGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGcc                                                     >  1:494093/1‑133 (MQ=255)                                                                            cAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCt                                             >  2:767781/1‑134 (MQ=255)                                                                              aTGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATgg                                                             >  1:178069/1‑116 (MQ=255)                                                                              aTGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATgg                                                             <  2:178069/116‑1 (MQ=255)                                                                                   ggCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCg                                       >  1:1029351/1‑133 (MQ=255)                                                                                      aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgcg                                                                         <  1:897914/96‑1 (MQ=255)                                                                                      aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgcg                                                                         <  2:405030/96‑1 (MQ=255)                                                                                      aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgcg                                                                         >  1:405030/1‑96 (MQ=255)                                                                                      aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgcg                                                                         >  2:897914/1‑96 (MQ=255)                                                                                      aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgc                                                                          <  2:330365/95‑1 (MQ=255)                                                                                      aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgc                                                                          >  1:330365/1‑95 (MQ=255)                                                                                      aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATg                                   >  1:762727/1‑134 (MQ=255)                                                                                      aTGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATg                                   >  2:1036045/1‑134 (MQ=255)                                                                                        gggCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgc                                                                          >  1:836036/1‑93 (MQ=255)                                                                                        gggCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAgc                                                                          <  2:836036/93‑1 (MQ=255)                                                                                         ggCCGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCt                                 <  1:1063787/133‑1 (MQ=255)                                                                                           ccGCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGgc                              >  2:83430/1‑134 (MQ=255)                                                                                             gCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAg                                                                               <  1:372445/83‑1 (MQ=255)                                                                                             gCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAg                                                                               >  2:372445/1‑83 (MQ=255)                                                                                             gCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGgcgc                            >  1:1093786/1‑134 (MQ=255)                                                                                             gCATGCTGGCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGgcgc                            >  2:201673/1‑134 (MQ=255)                                                                                                     gCATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCac                     <  1:981392/133‑1 (MQ=255)                                                                                                      cATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCacga                   <  1:1036045/134‑1 (MQ=255)                                                                                                      cATTGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCacga                   <  1:83430/134‑1 (MQ=255)                                                                                                        ttGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCAccg                                                                                                               <  1:674291/40‑1 (MQ=255)                                                                                                        ttGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCAccg                                                                                                               >  2:674291/1‑40 (MQ=255)                                                                                                        ttGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCAt                                                               <  2:1091727/88‑1 (MQ=255)                                                                                                        ttGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCAt                                                               >  1:1091727/1‑88 (MQ=255)                                                                                                        ttGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCacgacg                 >  2:968257/1‑134 (MQ=255)                                                                                                        ttGCTCGACGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCacgacg                 >  2:1046235/1‑134 (MQ=255)                                                                                                               aCGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATg                                   >  2:312273/1‑109 (MQ=255)                                                                                                               aCGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATg                                   <  1:312273/109‑1 (MQ=255)                                                                                                               aCGTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCACGACGCGCCGTa          >  1:109253/1‑134 (MQ=255)                                                                                                                 gTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTc                                                                    >  1:1140837/1‑74 (MQ=255)                                                                                                                 gTGCTGGAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTc                                                                    <  2:1140837/74‑1 (MQ=255)                                                                                                                       gAAAACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCACGACGCGCCGTATCGCCGTg  <  2:1029351/134‑1 (MQ=255)                                                                                                                          |                                                                                                                                   GGCCGCGCCCGCCTTCCTCGCGCGCCTGGCTGGCCGCACGGTGCGCCAGGGCGTGCCGGTGCCGGGCACGGTGCTCAATGAAGGCATGGGCCGCATGCTGGCATTGCTCGACGTGCTGGAATACTATCCGGGCCGCGGCACCGCCGTGCCCGCTGAAGCGATGGAGGTGTTGCAGTGAGCGCCCTCGCCATGGACCAGGCCGAGAGCCTGCGCCGGATGCTGGCGCCGCGCACGACGCGCCGTATCGCCGTG  >  AM260480/293286‑293537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑