Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	978,518	A→G	S117G (AGC→GGC)	h16_B0862 →	cytosine deaminase or related metal‑dependent hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	978,518	0	A	G	100.0%	139.7 / NA	36	S117G (AGC→GGC)	h16_B0862	cytosine deaminase or related metal‑dependent hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/17);  total (19/17)

CCGCGCATGCTGCTGATGCCCGGCATGATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAAGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCGTGGTCGATGCCGGCACGCGCAGCGACGCCGGGCTGGATGCGGTCGCGCGC  >  AM260480/978395‑978646                                                                                                                            |                                                                                                                                 ccgcGCATGCTGCTGATGCCCGGCATGATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTg                                                                                                                          <  1:531752/132‑1 (MQ=255)   gcgcATGCTGCTGATGCCCGGCATGATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAgg                                                                                                                      <  2:901618/134‑1 (MQ=255)                  gCCCGGCATGATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGc                                                                                                                     <  1:134617/120‑1 (MQ=255)                  gCCCGGCATGATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGc                                                                                                                     >  2:134617/1‑120 (MQ=255)                           gATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTcc                                                                                               >  2:835318/1‑133 (MQ=255)                                acacGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAgg                                                                                                                      <  2:915208/105‑1 (MQ=255)                                acacGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAgg                                                                                                                      >  1:915208/1‑105 (MQ=255)                                          ccaccTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTg                                                                                >  1:1038019/1‑133 (MQ=255)                                            accTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTgg                                                                               >  2:151458/1‑132 (MQ=255)                                              cTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAgg                                                                                                                      <  1:361634/91‑1 (MQ=255)                                              cTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAgg                                                                                                                      >  2:361634/1‑91 (MQ=255)                                               tGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAgcg                                                                          >  2:714223/1‑134 (MQ=255)                                                  cGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTg                                                                       <  1:1022677/134‑1 (MQ=255)                                                    cAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTgcg                                                                     >  1:565426/1‑134 (MQ=255)                                                    cAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTgcg                                                                     >  2:1067940/1‑134 (MQ=255)                                                      gTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGcgcg                                                                   >  2:984981/1‑134 (MQ=255)                                                      gTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGcgcg                                                                   <  1:835318/134‑1 (MQ=255)                                                         gTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGcc                                                                                                                            >  1:519697/1‑74 (MQ=255)                                                         gTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGcc                                                                                                                            <  2:519697/74‑1 (MQ=255)                                                           tCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGc                                                                                                                     <  1:1039426/79‑1 (MQ=255)                                                           tCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGc                                                                                                                     >  2:1039426/1‑79 (MQ=255)                                                                     gCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCg                                                    <  2:1038019/134‑1 (MQ=255)                                                                                  gAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAggca                                                                                                                               >  2:351015/1‑46 (MQ=255)                                                                                  gAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAggca                                                                                                                               <  1:351015/46‑1 (MQ=255)                                                                                  gAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTgtcgtc                                                                                                >  1:613491/1‑77 (MQ=255)                                                                                  gAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTgtcgtc                                                                                                <  2:613491/77‑1 (MQ=255)                                                                                      cATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCGTGGTCGATGCCGGCAcg                                   <  1:268325/134‑1 (MQ=255)                                                                                       aTCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCGTGGTCGATGCCGGCAcgc                                  <  1:860257/134‑1 (MQ=255)                                                                                                  tCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCGTGGTCGATGCCGGCACGCGCAGCg                            <  2:657277/129‑1 (MQ=255)                                                                                                  tCCGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCGTGGTCGATGCCGGCACGCGCAGCg                            >  1:657277/1‑129 (MQ=255)                                                                                                    cGGCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCGTGGTCGATGCCGGCACGCGCAGCGACGCCgg                     >  2:443831/1‑134 (MQ=255)                                                                                                     ggCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCggcggc                                                              <  2:421832/92‑1 (MQ=255)                                                                                                     ggCGCGTGTGGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCggcggc                                                              >  1:421832/1‑92 (MQ=255)                                                                                                             tgGGTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCGTGGTCGATGCCGGCACGCGCAGCGACGCCGGGCTGGATGc            >  1:51353/1‑134 (MQ=255)                                                                                                               ggTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCGTGGTCGATGCCGGCACGCGCAGCg                            <  2:664880/116‑1 (MQ=255)                                                                                                               ggTGCCGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCGTGGTCGATGCCGGCACGCGCAGCg                            >  1:664880/1‑116 (MQ=255)                                                                                                                    cGCTGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCGTGGTCGATGCCGGCACGCGCAGCGACGCCGGGCTGGATGCGGTcgcg     <  2:565426/134‑1 (MQ=255)                                                                                                                       tGGAAGGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCGTGGTCGATGCCGGCACGCGCAGCGACGCCGGGCTGGATGCGGTcgcgcgc  >  1:136506/1‑134 (MQ=255)                                                                                                                            |                                                                                                                                 CCGCGCATGCTGCTGATGCCCGGCATGATCGACACGCACCACCACCTGACGCAGTCGTTCGGCAAGTCGCTGGTGTTCGGCGAACCATCGGAGATCTTCCGGCGCGTGTGGGTGCCGCTGGAAAGCAGCCTGAAGGCGGAGCACCTGTACCTGTCGTCCAAGCTGGCGGCGCTGGAAGCGCTGCGCGGCGGCTTCACCACCGTGGTCGATGCCGGCACGCGCAGCGACGCCGGGCTGGATGCGGTCGCGCGC  >  AM260480/978395‑978646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑