Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AY305378	682	C→A	P190T (CCG→ACG)	hoxK →	membrane‑bound [NiFe] hydrogenase small subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AY305378	682	0	C	A	100.0%	136.8 / NA	36	P190T (CCG→ACG)	hoxK	membrane‑bound [NiFe] hydrogenase small subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/18);  total (18/18)

CAAGGATGCCAAGGCCATTATCTCCTGGGGTTCCTGCGCATCCTGGGGATGCGTGCAGGCAGCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCCCGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCGAAGATGTTCTATAGCCAGCGCATCCAC  >  AY305378/552‑804                                                                                                                                   |                                                                                                                           cAAGGATGCCAAGGCCATTATCTCCTGGGGTTCCTGCGCATCCTGGGGATGCGTGCAGGCAGCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACgg                                                                                                                         >  1:1041028/1‑134 (MQ=255) cAAGGATGCCAAGGCCATTATCTCCTGGGGTTCCTGCGCATCCTGGGGATGCGTGCAGGCAGCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACgg                                                                                                                         >  1:111583/1‑134 (MQ=255) cAAGGATGCCAAGGCCATTATCTCCTGGGGTTCCTGCGCATCCTGGGGATGCGTGCAGGCAGCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACgg                                                                                                                         >  1:985059/1‑134 (MQ=255)             ggCCATTATCTCCTGGGGTTCCTGCGCATCCTGGGGATGCGTGCAGGCAGCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGa                                                                                                             >  1:849921/1‑134 (MQ=255)                            gggTTCCTGCGCATCCTGGGGATGCGTGCAGGCAGCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGtgatg                                                                                               <  2:696577/133‑1 (MQ=255)                            gggTTCCTGCGCATCCTGGGGATGCGTGCAGGCAGCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGtgatg                                                                                               <  2:111583/133‑1 (MQ=255)                                   tGCGCATCCTGGGGATGCGTGCAGGCAGCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGgtgt                                                                                       >  2:352227/1‑134 (MQ=255)                                                        caggcagCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACgg                                                                                           <  2:570499/109‑1 (MQ=255)                                                        caggcagCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACgg                                                                                           >  1:570499/1‑109 (MQ=255)                                                                  aaCCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTAtt                                                        >  2:53608/1‑134 (MQ=255)                                                                  aaCCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTAtt                                                        >  2:1081509/1‑134 (MQ=255)                                                                         tCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAAc                                                 <  1:352227/134‑1 (MQ=255)                                                                             aCTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTTATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGa                                             >  1:956883/1‑134 (MQ=255)                                                                                  ggCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTcc                                                      <  2:971454/120‑1 (MQ=255)                                                                                  ggCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTcc                                                      >  1:971454/1‑120 (MQ=255)                                                                                  ggCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGa                                         >  2:452753/1‑133 (MQ=255)                                                                                    ccACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAg                                      <  2:1041028/134‑1 (MQ=255)                                                                                       cacCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTTATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGt                                   <  2:956883/134‑1 (MQ=255)                                                                                       cacCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCa                                                                      >  1:640053/1‑99 (MQ=255)                                                                                       cacCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCa                                                                      <  2:640053/99‑1 (MQ=255)                                                                                       cacCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTAGTCCCGAACTGGATCGACAGGGt                                   <  2:963554/134‑1 (MQ=255)                                                                                            gTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGcc                              >  1:107135/1‑134 (MQ=255)                                                                                            gTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGcc                              <  1:1081509/134‑1 (MQ=255)                                                                                            gTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGcc                              <  1:143779/134‑1 (MQ=255)                                                                                            gTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGcc                              <  1:53608/134‑1 (MQ=255)                                                                                               cacaAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCGa                            <  1:452753/133‑1 (MQ=255)                                                                                               cacaAGGTGATCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCGa                            <  2:106518/133‑1 (MQ=255)                                                                                                        aTCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGgtg                                                                                        >  2:692369/1‑64 (MQ=255)                                                                                                        aTCACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGgtg                                                                                        <  1:692369/64‑1 (MQ=255)                                                                                                          cACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACgg                                                                                           >  2:921067/1‑59 (MQ=255)                                                                                                          cACCGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACgg                                                                                           <  1:921067/59‑1 (MQ=255)                                                                                                             cGACAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCGAAGATGTTCTATAGc              >  2:1162763/1‑133 (MQ=255)                                                                                                               aCAAGCCGATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCGAAGATGTTCTATAGCCAg           >  1:817264/1‑134 (MQ=255)                                                                                                                      gATTATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCGAAGATGTTCTATAGCCAGCGCATcc    <  2:849921/134‑1 (MQ=255)                                                                                                                        ttATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCGAAGATGTTCTATAGCCAGCGCATCCAc  >  1:348736/1‑134 (MQ=255)                                                                                                                        ttATCAAGGTCACGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCGAAGATGTTCTATAGCCAGCGCATCCAc  <  1:1162763/134‑1 (MQ=255)                                                                                                                                   |                                                                                                                           CAAGGATGCCAAGGCCATTATCTCCTGGGGTTCCTGCGCATCCTGGGGATGCGTGCAGGCAGCCAAACCTAATCCCACTCAGGCCACACCGGTTCACAAGGTGATCACCGACAAGCCGATTATCAAGGTCCCGGGGTGCCCTCCGATTGCCGAAGTGATGACGGGTGTCATTACCTACATGCTCACCTTCGATCGTATTCCCGAACTGGATCGACAGGGTCGGCCGAAGATGTTCTATAGCCAGCGCATCCAC  >  AY305378/552‑804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑