| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260479 | 619,859 | G→T | H108N (CAC→AAC) | acrA1 ← | Cation/multidrug efflux system, mebrane‑fusion component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260479 | 619,859 | 0 | G | T | 100.0% | 115.0 / NA | 31 | H108N (CAC→AAC) | acrA1 | Cation/multidrug efflux system, mebrane‑fusion component |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (14/17); total (14/17) | |||||||||||
GGAACGGGCGCGGGTCGATCTCGGCCAGCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTGGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGTGGCGACCACCACGGGCGAGCGCGGCATGCCGGGGCCGCCGGCGCCACCCCGCGCGCCCG > AM260479/619730‑619963 | ggAACGGGCGCGGGTCGATCTCGGCCAGCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGcc > 2:1042425/1‑133 (MQ=255) gAACGGGCGCGGGTCGATCTCGGCCAGCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGcc > 2:417715/1‑132 (MQ=255) gAACGGGCGCGGGTCGATCTCGGCCAGCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGcc < 1:301546/132‑1 (MQ=255) gAACGGGCGCGGGTCGATCTCGGCCAGCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGcc > 1:531974/1‑132 (MQ=255) gcgcGGGTCGATCTCGGCCAGCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGt < 2:527749/134‑1 (MQ=255) cgGGTCGATCTCGGCCAGCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGa < 2:531974/134‑1 (MQ=255) cgGGTCGATCTCGGCCAGCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGa < 2:1039846/134‑1 (MQ=255) gggTCGATCTCGGCCAGCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAg > 1:219098/1‑134 (MQ=255) ggTCGATCTCGGCCAGCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGc < 1:36353/134‑1 (MQ=255) gCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGcc > 1:468862/1‑106 (MQ=255) gCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGcc < 2:468862/106‑1 (MQ=255) tCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCtt > 2:365314/1‑134 (MQ=255) ccGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGc < 2:615278/134‑1 (MQ=255) gACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGTg < 1:365314/133‑1 (MQ=255) aCCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGTgg < 1:261940/133‑1 (MQ=255) ccttGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGTGGCGACCACCACggg < 2:219098/133‑1 (MQ=255) ttGAGCAGTGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGcc > 1:989274/1‑62 (MQ=255) ttGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGcc < 2:989274/62‑1 (MQ=255) ttGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGcc > 2:760797/1‑62 (MQ=255) ttGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGcc < 1:760797/62‑1 (MQ=255) ttGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGa > 2:538399/1‑73 (MQ=255) ttGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGa < 1:538399/73‑1 (MQ=255) ttGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGc < 1:908770/98‑1 (MQ=255) ttGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGc > 2:908770/1‑98 (MQ=255) ttGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGTGGCGACCACCACGGGCGAGCGCGGCATGcc > 1:1137426/1‑134 (MQ=255) ccGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGa < 1:957509/61‑1 (MQ=255) ccGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGa > 2:957509/1‑61 (MQ=255) cacCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGa < 2:257760/56‑1 (MQ=255) cacCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGa > 1:257760/1‑56 (MQ=255) cACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGTGGCGACCACCACGGGCGAGCGCGGCATGCCGGGGCCGCCGGCGCCACCCCGCGCGCCCg < 1:980072/134‑1 (MQ=255) cACGGTGACGTTGGACACCGGGGTCACGTTGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGTGGCGACCACCACGGGCGAGCGCGGCATGCCGGGGCCGCCGGCGCCACCCCGCGCGCCCg > 2:980072/1‑134 (MQ=255) | GGAACGGGCGCGGGTCGATCTCGGCCAGCACGTCGCCGGCCTTGACCATCTGGCCTTCCTTGAACAGCACCTTGAGCAGCGGGCCGGACACCTGCGCGCGCACGGTGACGTTGGACACCGGGGTCACGTGGCCCAGGCCGTTGAGCACCACATCCATATCGCGCTTGGCCACGGTGGCGACCACCACGGGCGAGCGCGGCATGCCGGGGCCGCCGGCGCCACCCCGCGCGCCCG > AM260479/619730‑619963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |