Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	208,932	A→C	H322Q (CAT→CAG)	h16_A0195 ←	Putative GTPase (G3E family)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	208,932	0	A	C	100.0%	265.8 / NA	68	H322Q (CAT→CAG)	h16_A0195	Putative GTPase (G3E family)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (33/35);  total (33/35)

CAGGCAGTTCTCCAGGCCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCATGGAACACCACTTTGCGGTCAACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGG  >  AM260479/208803‑209013                                                                                                                                  |                                                                                  caggcAGTTCTCCAGGCCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTgg                                                                                >  2:899903/1‑133 (MQ=255)        ttCTCCAGGCCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAAcaccac                                                                        >  1:168774/1‑134 (MQ=255)             cAGGCCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGc                                                                   >  1:678295/1‑134 (MQ=255)                  ccTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                              <  1:899903/134‑2 (MQ=255)                      gAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTccac                                                            <  1:234854/132‑4 (MQ=255)                      gAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTccac                                                            <  2:678295/132‑4 (MQ=255)                      gAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTccac                                                            <  2:168774/132‑4 (MQ=255)                      gAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCcaca                                                           <  2:781743/133‑1 (MQ=255)                         gATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTccac                                                            <  2:958631/129‑4 (MQ=255)                         gATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTccac                                                            >  1:958631/1‑126 (MQ=255)                         gATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGt                                                       >  1:949686/1‑134 (MQ=255)                                tCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTc                                                               <  1:270668/119‑1 (MQ=255)                                tCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTc                                                               >  2:270668/1‑119 (MQ=255)                                  gcgGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTc                                                               >  2:110564/1‑117 (MQ=255)                                  gcgGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTc                                                               <  1:110564/117‑1 (MQ=255)                                  gcgGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCatata                                                >  1:435352/1‑132 (MQ=255)                                    ggggCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCatatat                                               <  1:34618/131‑1 (MQ=255)                                    ggggCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCatatat                                               >  2:34618/1‑131 (MQ=255)                                     gggCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTc                                                      >  1:295575/1‑123 (MQ=255)                                     gggCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTc                                                      <  2:295575/123‑1 (MQ=255)                                       gCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTc                                                               <  1:469583/112‑1 (MQ=255)                                       gCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTc                                                               >  2:469583/1‑112 (MQ=255)                                         aGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGcc                                       <  2:586165/134‑1 (MQ=255)                                                    gATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCatat                                                 >  2:658698/1‑113 (MQ=255)                                                    gATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCatat                                                 <  1:658698/113‑1 (MQ=255)                                                    gATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAgcg                              >  2:23842/1‑132 (MQ=255)                                                         acacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                              <  2:451541/95‑2 (MQ=255)                                                         acacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                              >  1:451541/1‑94 (MQ=255)                                                         acacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGt                                            <  2:486507/113‑1 (MQ=255)                                                         acacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGt                                            >  1:486507/1‑113 (MQ=255)                                                         acacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCAATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc                                                         >  2:498335/1‑100 (MQ=255)                                                         acacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCAATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc                                                         <  1:498335/100‑1 (MQ=255)                                                         acacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTACCCATCAGCTGGTGGACCCCCT‑GAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGtt                      >  2:782417/1‑134 (MQ=255)                                                          cacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                              >  2:322136/1‑93 (MQ=255)                                                          cacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                              <  1:322136/94‑2 (MQ=255)                                                          cacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGcagcag                        <  2:474640/132‑1 (MQ=255)                                                          cacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGcagcag                        >  1:474640/1‑132 (MQ=255)                                                          cacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTACCCATCAGCTGGTGGACCCCCT‑GAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGttt                     <  1:782417/134‑1 (MQ=255)                                                           acCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGttt                     <  2:949686/134‑1 (MQ=255)                                                                 cgcgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAAcaccac                                                                        <  2:913879/77‑1 (MQ=255)                                                                 cgcgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAAcaccac                                                                        >  1:913879/1‑77 (MQ=255)                                                                   cgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAAcacca                                                                         <  1:1157229/74‑1 (MQ=255)                                                                   cgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAAcacca                                                                         >  2:1157229/1‑74 (MQ=255)                                                                   cgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                              >  2:564603/1‑84 (MQ=255)                                                                   cgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                              <  1:564603/85‑2 (MQ=255)                                                                   cgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTc                                                      >  2:330637/1‑93 (MQ=255)                                                                   cgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTc                                                      <  1:330637/93‑1 (MQ=255)                                                                   cgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGt             <  1:23842/134‑1 (MQ=255)                                                                      gCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                              >  1:605026/1‑81 (MQ=255)                                                                      gCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                              <  2:605026/82‑2 (MQ=255)                                                                      gCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGttt                     >  1:913903/1‑123 (MQ=255)                                                                      gCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGttt                     <  2:913903/123‑1 (MQ=255)                                                                        ccGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTccac                                                            >  1:933659/1‑79 (MQ=255)                                                                        ccGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTccac                                                            <  2:933659/82‑4 (MQ=255)                                                                           gggTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAAcacca                                                                         >  1:812928/1‑66 (MQ=255)                                                                           gggTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAAcacca                                                                         <  2:812928/66‑1 (MQ=255)                                                                                      tccCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCacac                                                          >  2:341602/1‑70 (MQ=255)                                                                                      tccCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCacac                                                          <  1:341602/70‑1 (MQ=255)                                                                                              ggCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGttt                     >  2:548716/1‑99 (MQ=255)                                                                                              ggCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGttt                     <  1:548716/99‑1 (MQ=255)                                                                                                cgcCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                              >  2:801872/1‑55 (MQ=255)                                                                                                cgcCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                              <  1:801872/56‑2 (MQ=255)                                                                                                  ccGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCtt                                    <  1:12426/80‑1 (MQ=255)                                                                                                  ccGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCtt                                    >  2:12426/1‑80 (MQ=255)                                                                                                      tGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                              <  1:509308/50‑2 (MQ=39)                                                                                                      tGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                              >  2:509308/1‑49 (MQ=39)                                                                                                                          tggACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAgg  <  2:711965/90‑1 (MQ=255)                                                                                                                          tggACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAgg  >  1:711965/1‑90 (MQ=255)                                                                                                                                  |                                                                                  CAGGCAGTTCTCCAGGCCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCATGGAACACCACTTTGCGGTCAACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGG  >  AM260479/208803‑209013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑