Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	916,512	T→C	V341A (GTG→GCG)	h16_A0836 →	predicted exporter of the RND superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	916,512	0	T	C	100.0%	119.7 / NA	33	V341A (GTG→GCG)	h16_A0836	predicted exporter of the RND superfamily
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/17);  total (16/17)

TCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGTGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGATGTTGCCGGTG  >  AM260479/916392‑916624                                                                                                                         |                                                                                                                 tCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACg                                                                                                     >  1:899070/1‑134 (MQ=255) tCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACg                                                                                                     >  2:393816/1‑134 (MQ=255)           cAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCa                                                                                           >  2:507728/1‑134 (MQ=255)             aCTTTATCGTCGGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatc                                                                                         >  2:249165/1‑134 (MQ=255)             aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCt                                                                                                             <  2:22056/114‑1 (MQ=255)             aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCt                                                                                                             >  1:22056/1‑114 (MQ=255)             aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCa                                                                                                       <  1:295003/120‑1 (MQ=255)             aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCa                                                                                                       >  2:295003/1‑120 (MQ=255)             aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatc                                                                                         <  2:899070/134‑1 (MQ=255)             aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatc                                                                                         <  1:161274/134‑1 (MQ=255)             aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCa                                                                                           <  2:644757/132‑1 (MQ=255)             aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCa                                                                                           >  1:644757/1‑132 (MQ=255)                      tCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatcccgatccc                                                                                 >  2:269149/1‑133 (MQ=255)                          cgAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCt                                                                                                             <  1:435912/101‑1 (MQ=255)                          cgAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCt                                                                                                             >  2:435912/1‑101 (MQ=255)                          cgAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCgatga                                                                            >  2:963671/1‑134 (MQ=255)                            aGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATc                                                                          >  1:1096519/1‑134 (MQ=255)                                      gggTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTgg                                                                <  2:523726/134‑1 (MQ=255)                                           ccGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGAt                                                            <  1:391987/133‑1 (MQ=255)                                                  cGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACcgcg                                                     <  1:494119/133‑1 (MQ=255)                                                    ccATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGc                                                   >  1:465578/1‑133 (MQ=255)                                                     cATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCa                                                 >  1:471202/1‑134 (MQ=255)                                                       ttGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATc                                               <  1:249165/134‑1 (MQ=255)                                                          ccgccCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTAGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCgg                                             <  2:465578/133‑1 (MQ=255)                                                                 gcgcAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGc                                      >  1:530225/1‑133 (MQ=255)                                                                           aCCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGt                           <  2:1151699/134‑1 (MQ=255)                                                                           aCCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGt                           <  1:507728/134‑1 (MQ=255)                                                                           aCCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGt                           <  2:530225/134‑1 (MQ=255)                                                                                   gctgATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCt                                                                                                             <  1:733880/44‑1 (MQ=255)                                                                                   gctgATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCt                                                                                                             >  2:733880/1‑44 (MQ=255)                                                                                             ggATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCa                                                                                                       >  2:1040626/1‑40 (MQ=255)                                                                                             ggATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCa                                                                                                       <  1:1040626/40‑1 (MQ=25)                                                                                                      gcgcTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGATGTTGCCGGTg  <  1:963671/132‑1 (MQ=255)                                                                                                                         |                                                                                                                 TCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGTGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGATGTTGCCGGTG  >  AM260479/916392‑916624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑