Predicted mutation
evidence seq id position mutation annotation gene description
RA AM260479 208,953 A→C V315V (GTT→GTG)  h16_A0195 ← Putative GTPase (G3E family)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM260479208,9530AC100.0% 143.1 / NA 37V315V (GTT→GTG) h16_A0195Putative GTPase (G3E family)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/18);  total (19/18)

GAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCATGGAACACCACTTTGCGGTCAACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGG  >  AM260479/208824‑209067
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gAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCcaca                                                                                                                 <  2:781743/133‑1 (MQ=255)
   gATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGt                                                                                                             >  1:949686/1‑134 (MQ=255)
            gcgGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCatata                                                                                                      >  1:435352/1‑132 (MQ=255)
              ggggCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCatatat                                                                                                     >  2:34618/1‑131 (MQ=255)
              ggggCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCatatat                                                                                                     <  1:34618/131‑1 (MQ=255)
               gggCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTc                                                                                                            <  2:295575/123‑1 (MQ=255)
               gggCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTc                                                                                                            >  1:295575/1‑123 (MQ=255)
                   aGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGcc                                                                                             <  2:586165/134‑1 (MQ=255)
                              gATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCatat                                                                                                       >  2:658698/1‑113 (MQ=255)
                              gATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCatat                                                                                                       <  1:658698/113‑1 (MQ=255)
                              gATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAgcg                                                                                    >  2:23842/1‑132 (MQ=255)
                                   acacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGt                                                                                                  >  1:486507/1‑113 (MQ=255)
                                   acacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGt                                                                                                  <  2:486507/113‑1 (MQ=255)
                                   acacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCAATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc                                                                                                               <  1:498335/100‑1 (MQ=255)
                                   acacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCAATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc                                                                                                               >  2:498335/1‑100 (MQ=255)
                                   acacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTACCCATCAGCTGGTGGACCCCCT‑GAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGtt                                                                            >  2:782417/1‑134 (MQ=255)
                                    cacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGcagcag                                                                              >  1:474640/1‑132 (MQ=255)
                                    cacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGcagcag                                                                              <  2:474640/132‑1 (MQ=255)
                                    cacCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTACCCATCAGCTGGTGGACCCCCT‑GAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGttt                                                                           <  1:782417/134‑1 (MQ=255)
                                     acCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGttt                                                                           <  2:949686/134‑1 (MQ=255)
                                             cgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTc                                                                                                            <  1:330637/93‑1 (MQ=255)
                                             cgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTc                                                                                                            >  2:330637/1‑93 (MQ=255)
                                             cgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGt                                                                   <  1:23842/134‑1 (MQ=255)
                                                gCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGttt                                                                           >  1:913903/1‑123 (MQ=255)
                                                gCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGttt                                                                           <  2:913903/123‑1 (MQ=255)
                                                                tccCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCacac                                                                                                                >  2:341602/1‑70 (MQ=255)
                                                                tccCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCacac                                                                                                                <  1:341602/70‑1 (MQ=255)
                                                                        ggCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGttt                                                                           >  2:548716/1‑99 (MQ=255)
                                                                        ggCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGttt                                                                           <  1:548716/99‑1 (MQ=255)
                                                                            ccGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCtt                                                                                          >  2:12426/1‑80 (MQ=255)
                                                                            ccGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCtt                                                                                          <  1:12426/80‑1 (MQ=255)
                                                                                                    tggACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAgg                                                        >  1:711965/1‑90 (MQ=255)
                                                                                                    tggACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAgg                                                        <  2:711965/90‑1 (MQ=255)
                                                                                                                 acacCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCgg  >  1:360307/1‑131 (MQ=255)
                                                                                                                      acTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCg   <  2:306869/125‑1 (MQ=255)
                                                                                                                      acTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCg   >  1:306869/1‑125 (MQ=255)
                                                                                                                         ttGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTa                        >  1:673528/1‑101 (MQ=255)
                                                                                                                         ttGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTa                        <  2:673528/101‑1 (MQ=255)
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GAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCATGGAACACCACTTTGCGGTCAACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAATTCCTCCAGCTTGCCGTAGTGGAAGGGCTTGTCGCTGCGG  >  AM260479/208824‑209067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑