Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	1,081,530	T→C	L30L (TTG→CTG)	h16_A0990 →	outer membrane protein and related peptidoglycan‑associated (lipo)proteins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	1,081,530	0	T	C	100.0%	92.7 / NA	25	L30L (TTG→CTG)	h16_A0990	outer membrane protein and related peptidoglycan‑associated (lipo)proteins
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/13);  total (12/13)

GAAGCCTCGGCCGGCGGCACGCAAGG‑CCCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCTTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGCAGCAGTTCATGGCCTACGGCAAGCCCGAGGCCGCGGCCGAC  >  AM260479/1081408‑1081628                                                                                                                            |                                                                                                   gAAGCCTCGGCCGGCGGCACGCAAGGCCCCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTAc                                                                                          <  1:365853/134‑1 (MQ=255)                   aCGCAAGGCCCCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCggg                                                                        >  1:421597/1‑134 (MQ=255)                         ggccccTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCa                                                                  <  1:1091291/131‑1 (MQ=255)                          gccccTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAg                                                                 <  1:885981/132‑1 (MQ=255)                                        aTCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGc                                                                            <  2:451222/109‑1 (MQ=255)                                        aTCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGc                                                                            >  1:451222/1‑109 (MQ=255)                                          cTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGc                                                        >  1:108460/1‑127 (MQ=255)                                          cTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGc                                                        >  2:585120/1‑127 (MQ=255)                                          cTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGc                                                        >  2:1076181/1‑127 (MQ=255)                                              gATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCt                                             <  1:585120/134‑1 (MQ=255)                                              gATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCt                                             <  2:423320/134‑1 (MQ=255)                                              gATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCt                                             <  1:703662/134‑1 (MQ=255)                                                         gCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGCAGCAGTTCa                                  >  2:156733/1‑134 (MQ=255)                                                                           cGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGc                                                                            <  2:950770/74‑1 (MQ=255)                                                                           cGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGc                                                                            >  1:950770/1‑74 (MQ=255)                                                                                     gTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGc                                                                            <  1:641388/64‑1 (MQ=255)                                                                                     gTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGc                                                                            >  2:641388/1‑64 (MQ=255)                                                                                      tGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGCAGCAGTTCATGGCCTACGGCAAGCCCGAGGCCGCGGcc     >  2:414659/1‑134 (MQ=255)                                                                                        ccGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGCAGCAGTTCATGGCCTACGGCAAGCCCGAGGCCGCGGCCg    <  1:336482/133‑1 (MQ=255)                                                                                        ccGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGCAGCAGTTCATGGCCTACGGCAAGCCCGAGGCCGCGGCCGa   >  2:847450/1‑134 (MQ=255)                                                                                         cGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGCAGCAGTTCATGGCCTACGGCAAGCCCGAGGCCGCGGCCGAc  <  1:414659/134‑1 (MQ=255)                                                                                                  aGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGCAGCAGTTCATGGCCTACGGCAAGCCCGAGGCCGCGGCCGAc  >  2:327993/1‑125 (MQ=255)                                                                                                  aGCCCGTCAAGGCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGCAGCAGTTCATGGCCTACGGCAAGCCCGAGGCCGCGGCCGAc  <  1:327993/125‑1 (MQ=255)                                                                                                            ggCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGcagcag                                      <  1:915127/79‑1 (MQ=255)                                                                                                            ggCCGGGGCCCTGCGCCTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGcagcag                                      >  2:915127/1‑79 (MQ=255)                                                                                                                            |                                                                                                   GAAGCCTCGGCCGGCGGCACGCAAGG‑CCCTGGACCGTGATCTACGATGCGGACCTGCCGGGGCAGGACGGCTACGATACCCGCGTGCCGCTGGCCCAGCCCGTCAAGGCCGGGGCCCTGCGCTTGACGCTTACCGGCAACCGCTGGCCGGGCGACCAGGACGAGGGCCAGATCGGCCTGCAGCAGTTCATGGCCTACGGCAAGCCCGAGGCCGCGGCCGAC  >  AM260479/1081408‑1081628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑