Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	1,167,791	T→C	intergenic (+238/‑365)	h16_A1070 → / → cyoA1	acyl dehydratase/bo3‑type quinol oxidase, subunit II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	1,167,791	0	T	C	100.0%	74.1 / NA	20	intergenic (+238/‑365)	h16_A1070/cyoA1	acyl dehydratase/bo3‑type quinol oxidase, subunit II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/9);  total (11/9)

GCCTGTCAAATGCTGCTTCGCGGATTTGTGCGTCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTATGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTACCCGGCCGCCGTACTGAGC  >  AM260479/1167668‑1167893                                                                                                                            |                                                                                                       gCCTGTCAAATGCTGCTTCGCGGATTTGTGCGTCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCt                                                                                              >  2:600876/1‑134 (MQ=255)          aTGCTGCTTCGCGGATTTGTGCGTCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTcccc                                                                                          <  1:1155498/129‑1 (MQ=255)          aTGCTGCTTCGCGGATTTGTGCGTCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTcccc                                                                                          >  2:1155498/1‑129 (MQ=255)                            gtgCGTCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCAt                                                                    >  1:641828/1‑133 (MQ=255)                               cGTCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGaa                                                                 <  2:641828/133‑1 (MQ=255)                                 tCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGc                                                              <  2:854090/134‑1 (MQ=255)                                          cGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTc                                                     <  2:1086278/134‑1 (MQ=255)                                                     aCAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCa                                           >  2:348096/1‑133 (MQ=255)                                                                      gTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAAc                         >  2:353641/1‑134 (MQ=255)                                                                       ttGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTcc                            >  2:1004549/1‑130 (MQ=255)                                                                       ttGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTcc                            <  1:1004549/130‑1 (MQ=255)                                                                       ttGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTT‑CCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGc                       >  2:7945/1‑134 (MQ=255)                                                                                   cTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTACCCGgccgc            >  1:378596/1‑134 (MQ=255)                                                                                        gttgcgttgcGGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTACCCGGCCGCCGTAc       >  1:576018/1‑134 (MQ=255)                                                                                         ttgcgttgcgGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTcc                            <  2:210195/112‑1 (MQ=255)                                                                                         ttgcgttgcgGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTcc                            >  1:210195/1‑112 (MQ=255)                                                                                            cgttgcgGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTACCCGGCCGCCGTACTGAg   <  1:348096/134‑1 (MQ=255)                                                                                             gttgcgGTACAGTCGCGCCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTACCCGGCCGCCGTACTGAGc  <  1:527694/134‑1 (MQ=255)                                                                                                             gcCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTACCCGgccgcc           >  1:64347/1‑109 (MQ=255)                                                                                                             gcCCACGATGTTTTACGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTACCCGgccgcc           <  2:64347/109‑1 (MQ=255)                                                                                                                            |                                                                                                       GCCTGTCAAATGCTGCTTCGCGGATTTGTGCGTCGATTGTCCGTTCTGCTGAACAGTGTGTTCTGTGCCGTTGGGTTTACCCCTAATGTTGCGTTGCGGTACAGTCGCGCCCACGATGTTTTATGTGTTGCGCTCCCCGGCCGAAAACCGCGCTTGCCATGAAGGCAAGTCCGTCGGGAAGTGCAAAAACCTCGTATTCCAACGCTACCCGGCCGCCGTACTGAGC  >  AM260479/1167668‑1167893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑