Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	1,744,688	G→C	G211A (GGC→GCC)	h16_A1608 →	conserved hypothetical protein containing von Willebrand factor (vWF) type A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	1,744,688	0	G	C	100.0%	93.3 / NA	25	G211A (GGC→GCC)	h16_A1608	conserved hypothetical protein containing von Willebrand factor (vWF) type A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (14/11);  total (14/11)

GACCCGCATCATGCCGGTGCCGGGGCGCGACATGGCACAGGCGCTGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGGCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGGGCGGCTATGTCGAACAG  >  AM260479/1744560‑1744800                                                                                                                                 |                                                                                                                 gACCCGCATCATGCCGGTGCCGGGGCGCGACATGGCACAGGCGCTGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAggg                                                                                                             >  1:822332/1‑134 (MQ=255)      gCATCATGCCGGTGCCGGGGCGCGACATGGCACAGGCGCTGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTc                                                                                                        <  1:121968/134‑1 (MQ=255)                  tgccggGGCGCGACATGGCACAGGCGCTGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCg                                                                                            <  1:1029991/134‑1 (MQ=255)                      ggggCGCGACATGGCACAGGCGCTGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGa                                                                                          >  2:737046/1‑132 (MQ=255)                      ggggCGCGACATGGCACAGGCGCTGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGa                                                                                          >  2:1024426/1‑132 (MQ=255)                            cgACATGGCACAGGCGCTGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGcc                                                                                  <  2:822332/134‑1 (MQ=255)                                             tGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGgtggtg                                                                 >  2:834302/1‑134 (MQ=255)                                             tGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGgtggtg                                                                 >  1:807221/1‑134 (MQ=255)                                             tGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGgtggtg                                                                 >  1:292039/1‑134 (MQ=255)                                                      ttGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATc                                                        <  2:807221/134‑1 (MQ=255)                                                      ttGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATc                                                        <  2:115333/134‑1 (MQ=255)                                                                  tGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCg                                                                       <  1:75658/107‑1 (MQ=255)                                                                  tGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCg                                                                       >  2:75658/1‑107 (MQ=255)                                                                  tGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATc                                                        <  2:755230/122‑1 (MQ=255)                                                                  tGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATc                                                        >  1:755230/1‑122 (MQ=255)                                                                    gcgcGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAgg                                           <  1:329531/133‑1 (MQ=255)                                                                             ccGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCt                                 >  1:772513/1‑134 (MQ=255)                                                                                      ggCACCATCCTGTTCCTGACTGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGc                        <  1:23710/134‑1 (MQ=255)                                                                                           cATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCaa                           <  2:1061750/126‑1 (MQ=255)                                                                                           cATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCaa                           >  1:1061750/1‑126 (MQ=255)                                                                                                  ttCCTGACCGACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGggcggc              <  2:228224/132‑1 (MQ=255)                                                                                                           gACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGGGCGGCTATGTCGaa     >  1:123212/1‑132 (MQ=255)                                                                                                           gACGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGGGCGGCTATGTCGaa     >  1:1035088/1‑132 (MQ=255)                                                                                                            aCGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGGGCGGCTATGTCGAACAg  >  2:1080381/1‑134 (MQ=255)                                                                                                            aCGGCGTGGACCCCGCCGCCGCCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGGGCGGCTATGTCGAACAg  >  2:339194/1‑134 (MQ=255)                                                                                                                                 |                                                                                                                 GACCCGCATCATGCCGGTGCCGGGGCGCGACATGGCACAGGCGCTGCGCACCATTGACGCCGACCTGGCGCGTGAGCCGGTGCCCGGCACCATCCTGTTCCTGACCGACGGCGTGGACCCCGCCGCCGGCAGGGCCTTCCGCGCGCAGGCGGACAGCGGCCGCAGCCAGCCGGTGGTGCTGGCCATCGGCACGGAACAGGGCGGGCCGCTGCGCAATGCAGCGGGCGGCTATGTCGAACAG  >  AM260479/1744560‑1744800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑