Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	342,407	T→C	L180L (TTG→CTG)	amtB →	ammonium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	342,407	0	T	C	100.0%	137.5 / NA	36	L180L (TTG→CTG)	amtB	ammonium transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/19);  total (17/19)

TGGCTACAGCTTTGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAATTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCACCTTCT  >  AM260479/342283‑342530                                                                                                                             |                                                                                                                            tGGCTACAGCTTTGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACt                                                                                                                    >  1:1114781/1‑134 (MQ=255)           tttGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTc                                                                                                            >  1:1056021/1‑132 (MQ=255)           tttGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTc                                                                                                            >  2:950947/1‑132 (MQ=255)           tttGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTc                                                                                                            >  1:39470/1‑132 (MQ=255)             tGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGt                                                                                                        >  2:763231/1‑134 (MQ=255)                   cACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGcc                                                                                                   <  2:173033/133‑1 (MQ=255)                         gggCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCt                                                                                            <  2:1114781/134‑1 (MQ=255)                             aaCGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCAt                                                                                        >  2:870874/1‑134 (MQ=255)                             aaCGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCAt                                                                                        >  1:138437/1‑134 (MQ=255)                              aCGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATc                                                                                       <  1:950947/134‑1 (MQ=255)                                  cttcttCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCt                                                                                   >  2:601912/1‑134 (MQ=255)                                                             cATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGcc                                                         <  2:1030710/133‑1 (MQ=255)                                                                 aaGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAAcg                                                    <  1:1046867/134‑1 (MQ=255)                                                                 aaGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAAcg                                                    <  2:216590/134‑1 (MQ=255)                                                                      gCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCt                                                                                            <  1:1069206/89‑1 (MQ=255)                                                                      gCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCt                                                                                            >  2:1069206/1‑89 (MQ=255)                                                                         gACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGcc                                                                                                   <  2:765066/79‑1 (MQ=255)                                                                         gACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGcc                                                                                                   >  1:765066/1‑79 (MQ=255)                                                                         gACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGt                                            >  2:874636/1‑134 (MQ=255)                                                                           cGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTtc                                          <  1:14713/134‑1 (MQ=255)                                                                                    ccGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTg                                 >  2:123781/1‑134 (MQ=255)                                                                                     cGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGc                                <  2:138437/134‑1 (MQ=255)                                                                                            gccACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGtt                         <  1:734397/134‑1 (MQ=255)                                                                                                ccTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCgtg                     <  1:870874/134‑1 (MQ=255)                                                                                                       cAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCtgt              <  2:431412/134‑1 (MQ=255)                                                                                                       cAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCtgt              <  1:1083651/134‑1 (MQ=255)                                                                                                         aGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGcc                                                                                                   <  1:1060171/47‑1 (MQ=255)                                                                                                         aGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGcc                                                                                                   >  2:1060171/1‑47 (MQ=255)                                                                                                              gtggtggtgCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCAc       >  2:382846/1‑134 (MQ=255)                                                                                                                   ggtgCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCACCTtct  >  1:261694/1‑134 (MQ=255)                                                                                                                    gtgCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCACCTtct  <  1:945678/133‑1 (MQ=255)                                                                                                                     tgCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCACCtt    >  1:894211/1‑130 (MQ=255)                                                                                                                     tgCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCACCtt    <  2:894211/130‑1 (MQ=255)                                                                                                                         ggAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTg                                                                                  <  2:956463/48‑1 (MQ=255)                                                                                                                         ggAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTg                                                                                  >  1:956463/1‑48 (MQ=255)                                                                                                                          gAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCt                                                                                            <  1:233728/37‑1 (MQ=255)                                                                                                                             |                                                                                                                            TGGCTACAGCTTTGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAATTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCACCTTCT  >  AM260479/342283‑342530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑