Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	2,016,501	T→C	V678V (GTT→GTC)	h16_A1857 →	Uncharacterized protein involved in exopolysaccharide biosynthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	2,016,501	0	T	C	100.0%	171.9 / NA	45	V678V (GTT→GTC)	h16_A1857	Uncharacterized protein involved in exopolysaccharide biosynthesis
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/25);  total (20/25)

TTGCTGCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTTTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGCTA  >  AM260479/2016373‑2016629                                                                                                                                 |                                                                                                                                 ttGCTGCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTc                                                                                                                               <  1:654278/132‑1 (MQ=255)                   aCCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg                                                                                                            <  1:1074350/133‑1 (MQ=255)                   aCCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg                                                                                                            <  1:214299/133‑1 (MQ=255)                    ccACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTc                                                                                                          <  1:948093/134‑1 (MQ=255)                       cacTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTcac                                                                                                        >  2:414775/1‑133 (MQ=255)                       cacTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTc                                                                                                          <  2:287179/131‑1 (MQ=255)                       cacTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTc                                                                                                          >  1:287179/1‑131 (MQ=255)                                 aCGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg                                                                                                            <  1:216871/119‑1 (MQ=255)                                 aCGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg                                                                                                            >  2:216871/1‑119 (MQ=255)                                 aCGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGaa                                                                                              >  1:320215/1‑133 (MQ=255)                                      ttGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGaa                                                                                              >  2:166951/1‑128 (MQ=255)                                      ttGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGaa                                                                                              >  1:565294/1‑128 (MQ=255)                                           ggCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATcgacg                                                                                    >  2:416771/1‑133 (MQ=255)                                                     aCATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg                                                                                                            <  2:640989/99‑1 (MQ=255)                                                     aCATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg                                                                                                            >  1:640989/1‑99 (MQ=255)                                                     aCATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACgcgc                                                                         >  1:1048856/1‑134 (MQ=255)                                                                    aCGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGcc                                                          >  2:665778/1‑134 (MQ=255)                                                                            ccATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTggcac                                                                                                                     >  1:824786/1‑67 (MQ=255)                                                                            ccATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTggcac                                                                                                                     <  2:824786/67‑1 (MQ=255)                                                                            ccATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGc                                                  <  1:459582/134‑1 (MQ=255)                                                                            ccATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGc                                                  >  2:431649/1‑134 (MQ=255)                                                                             cATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGcc                                                 <  2:484218/134‑1 (MQ=255)                                                                             cATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGcc                                                 <  2:801123/134‑1 (MQ=255)                                                                             cATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGcc                                                 <  1:255651/134‑1 (MQ=255)                                                                              aTTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACg                                                      <  2:135427/128‑1 (MQ=255)                                                                              aTTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACg                                                      >  1:135427/1‑128 (MQ=255)                                                                                                   gcTTGTGCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACg                           <  1:28370/134‑1 (MQ=255)                                                                                                        tgCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg                                                                                                            <  2:494223/48‑1 (MQ=255)                                                                                                        tgCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg                                                                                                            >  1:494223/1‑48 (MQ=255)                                                                                                        tgCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTc                      <  1:665778/134‑1 (MQ=255)                                                                                                         gCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg                                                                                                            >  1:34624/1‑47 (MQ=255)                                                                                                         gCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCggg                                                                                                            <  2:34624/47‑1 (MQ=255)                                                                                                         gCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTc                      <  2:876419/133‑1 (MQ=255)                                                                                                         gCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTc                      >  1:876419/1‑133 (MQ=255)                                                                                                         gCTGGGACGCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCa                     >  2:6952/1‑134 (MQ=255)                                                                                                                 gCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCAc             <  2:320215/134‑1 (MQ=255)                                                                                                                 gCATGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCAc             <  2:565294/134‑1 (MQ=255)                                                                                                                   aTGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAgg                                                            <  1:620686/85‑1 (MQ=255)                                                                                                                   aTGGCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAgg                                                            >  2:620686/1‑85 (MQ=255)                                                                                                                      gCGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCa        >  2:521939/1‑134 (MQ=255)                                                                                                                       cGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCAc       <  1:431649/134‑1 (MQ=255)                                                                                                                       cGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCAc       >  2:279083/1‑134 (MQ=255)                                                                                                                       cGGGCACTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCAc       <  1:279083/134‑1 (MQ=255)                                                                                                                            aCTGTCTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGCTa  <  1:166951/134‑1 (MQ=255)                                                                                                                            aCTGTCATCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGCTa  <  2:1048856/134‑1 (MQ=255)                                                                                                                                 |                                                                                                                                 TTGCTGCAGCCAGAACTGACCACACTTGTTCAACGCGTTGCCGGCCAGTACGACATGGTGGTGCTTGACGGACCGCCATTGCTGCCGGTGGCAGATGCGCTTGTGCTGGGACGCATGGCGGGCACTGTTTTCATCGTGGCACGGCACCGGGTCACCACGGTGGAACAGATCGACGAAAGCACGCGCCGGCTGGCGCAGGCCGACGTCGCCGTACGGGGCGTGATCTTCAACGACTTCACCGGCCACCTGCACCGCTA  >  AM260479/2016373‑2016629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑