Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	2,517,378	T→G	P77A (CCA→GCC)	zntA ←	putative heavy metal transporting P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	2,517,378	0	T	G	100.0%	57.2 / NA	16	P77A (CCA→GCC)	zntA	putative heavy metal transporting P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/8);  total (8/8)

GCGGACAAACTGGGCGCGCAACTCAGGGGTGTCGTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCTGGCGCATAGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACGGGCAGGCCG  >  AM260479/2517252‑2517486                                                                                                                               |                                                                                                             gcggACAAACTGGGCGCGCAACTCAGGGGTGTCGTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCAt                                                                                                       >  1:529815/1‑134 (MQ=255)                           gggTGTCGTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCaa                                                                             <  1:602716/134‑1 (MQ=255)                             gtgtCGTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAAcc                                                                           <  2:529815/134‑1 (MQ=255)                                    aGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCgcag                                                                    >  2:906092/1‑134 (MQ=255)                                                             gcgACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTcc                                            <  1:314283/133‑1 (MQ=255)                                                                  gggCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAacac                                                            >  1:446663/1‑112 (MQ=255)                                                                  gggCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAacac                                                            <  2:446663/112‑1 (MQ=255)                                                                                                       aTCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTcc                                            <  1:954188/91‑1 (MQ=255)                                                                                                       aTCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTcc                                            >  2:954188/1‑91 (MQ=255)                                                                                                       aTCCCCGTACAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACGGGCAGGCCg  >  1:56686/1‑133 (MQ=255)                                                                                                                cAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCcggcg                                                      <  1:404552/72‑1 (MQ=255)                                                                                                                cAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCcggcg                                                      <  1:705269/72‑1 (MQ=255)                                                                                                                cAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCcggcg                                                      >  2:404552/1‑72 (MQ=255)                                                                                                                cAGGTGCGCAAAGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCcggcg                                                      >  2:705269/1‑72 (MQ=255)                                                                                                                          aaGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACcgccgc                                                                      <  1:463726/46‑1 (MQ=37)                                                                                                                          aaGCCGGCCGCATGGAGGGTCTGCGCCAATGCCTGGCAACcgccgc                                                                      >  2:463726/1‑46 (MQ=37)                                                                                                                               |                                                                                                             GCGGACAAACTGGGCGCGCAACTCAGGGGTGTCGTAGGCGGCCCAGATCGGTTCGGCCTCGCGACGGGCGTCGTCGTCGATGGGGCGGGCGAAGCGGGCTTCATCCCCGTACAGGTGCGCAAAGCCTGGCGCATAGAGGGTCTGCGCCAATGCCTGGCAACCGCCGCAGCAGAACACCCGGCGGCTGCCGTCCAGCTCGGCGGCAAGCGGCTCGGTGTCCGTGACGGGCAGGCCG  >  AM260479/2517252‑2517486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑