Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	406,488	G→T	L70I (CTC→ATC)	h16_A0388 ←	ABC‑type transporter, ATPase component: HAAT family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	406,488	0	G	T	100.0%	72.4 / NA	20	L70I (CTC→ATC)	h16_A0388	ABC‑type transporter, ATPase component: HAAT family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/10);  total (10/10)

CTGCGGCAGGTACGACAGCCCCATGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGAGCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACGTCCTTGACCACCGTGCGCGAGCCATAGCGTTTCTTCAG  >  AM260479/406378‑406608                                                                                                               |                                                                                                                         cTGCGGCAGGTACGACAGCCCCATGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGc                                                                                                   >  1:13089/1‑134 (MQ=255)          gTACGACAGCCCCATGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGt                                                                                          <  2:74087/134‑1 (MQ=255)                       aTGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCcgcg                                                                                              >  1:543943/1‑117 (MQ=255)                       aTGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCcgcg                                                                                              >  1:685666/1‑117 (MQ=255)                       aTGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCcgcg                                                                                              <  2:685666/117‑1 (MQ=255)                       aTGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCcgcg                                                                                              <  2:543943/117‑1 (MQ=255)                       aTGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAg                                                                             >  1:140455/1‑134 (MQ=255)                       aTGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAg                                                                             >  1:732309/1‑134 (MQ=255)                       aTGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAg                                                                             >  1:850935/1‑134 (MQ=255)                       aTGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAg                                                                             >  2:245971/1‑134 (MQ=255)                                cgcTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGaccac                                                                    <  2:850935/134‑1 (MQ=255)                                                     cTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTcgag                                               <  1:115105/134‑1 (MQ=255)                                                        aTGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTcgagc                                              <  1:658585/132‑1 (MQ=255)                                                        aTGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTcgagc                                              <  2:140455/132‑1 (MQ=255)                                                                    tCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGaccac                                                                    <  2:1061754/98‑1 (MQ=255)                                                                    tCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGaccac                                                                    >  1:1061754/1‑98 (MQ=255)                                                                                  gCCTTCGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACGTCCTTGACCACCGTGCGCGAGc                  >  1:1103297/1‑134 (MQ=255)                                                                                       cGTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACGTCCTTGACCACCGTGCGCGAGCCAt               <  2:732309/132‑1 (MQ=255)                                                                                        gTCCAGCGCCACCAGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACGTCCTTGACCACCGTGCGCGAGCCATAg             <  2:1103297/133‑1 (MQ=255)                                                                                                  accaGGCCCACGATCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACGTCCTTGACCACCGTGCGCGAGCCATAGCGTTTCTTCAg  >  2:4659/1‑134 (MQ=255)                                                                                                               |                                                                                                                         CTGCGGCAGGTACGACAGCCCCATGCGCGCGCGCTCGTGGATCGGCAGGCCGCTGATGTGGTCGCCGTCGAGCACGATATCGCCTTCGTCCAGCGCCACCAGGCCCACGAGCATGTAGAAGGAGGTGGTCTTGCCCGCGCCGTTGGGCCCAAGCAGGCCGACCACCTCGCCGCTCTTCACGTCGAGCGAGACGTCCTTGACCACCGTGCGCGAGCCATAGCGTTTCTTCAG  >  AM260479/406378‑406608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑