Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	2,580,039	A→G	R155R (CGT→CGC)	h16_A2375 ←	Protein‑L‑isoaspartate carboxylmethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	2,580,039	0	A	G	100.0%	53.2 / NA	15	R155R (CGT→CGC)	h16_A2375	Protein‑L‑isoaspartate carboxylmethyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/7);  total (8/7)

TGCAGCGGGCGGATGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCACGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCGATGCCAGTCCCGGCTCGACGAACAGGTGGCGC  >  AM260479/2579911‑2580162                                                                                                                                 |                                                                                                                            tGCAGCGGGCGGATGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCgcag                                                                                                                        >  1:192409/1‑134 (MQ=255) tGCAGCGGGCGGATGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCgcag                                                                                                                        >  1:286613/1‑134 (MQ=255)                                     cgcgcgACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGAt                                                                                                               >  1:175659/1‑107 (MQ=255)                                     cgcgcgACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGAt                                                                                                               <  2:175659/107‑1 (MQ=255)                                                            ggCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATc                                                              >  1:732591/1‑133 (MQ=255)                                                            ggCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATc                                                              <  2:732591/133‑1 (MQ=255)                                                               cTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCgg                                                            >  1:223918/1‑132 (MQ=255)                                                                  gTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAgcg                                                       <  2:192409/134‑1 (MQ=255)                                                                                                          ggcgCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGcgcg                                                     >  1:1108040/1‑96 (MQ=255)                                                                                                          ggcgCGTCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGcgcg                                                     <  2:1108040/96‑1 (MQ=255)                                                                                                                tCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCg                                                                                              >  1:818760/1‑49 (MQ=255)                                                                                                                tCGGCGGCCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCg                                                                                              <  2:818760/49‑1 (MQ=255)                                                                                                                  ggcggcCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGccacca                                                                                                 <  1:816940/44‑1 (MQ=255)                                                                                                                  ggcggcCAGGCCCTCGCGCAGCAGCTCGATCATGCGCGccacca                                                                                                 >  2:816940/1‑44 (MQ=255)                                                                                                                       ccAGGCCCTCGCGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCGATGCCAGTCCCGGCTCGACGAACAGGTGgcgc  <  2:223918/134‑1 (MQ=255)                                                                                                                                 |                                                                                                                            TGCAGCGGGCGGATGCGCTCGATCGAGAAGACCTCGCGCGCGACCTGGCTCAGCACCGCGGCCTGGTAGCCGCAGCCGGTGCCGATCTCGAGCACGCGCTCCAGCGGCGCGTCGGCGGCCAGGCCCTCACGCAGCAGCTCGATCATGCGCGCCACCACCGACGGCTTGGAAATGGTCTGCTGGTGGCCGATCGGCAGCGCGGCGTCCTCATACGCCTGCGATGCCAGTCCCGGCTCGACGAACAGGTGGCGC  >  AM260479/2579911‑2580162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑