Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	2,616,505	C→G	A45G (GCC→GGC)	h16_A2404 →	predicted esterase of the alpha‑beta hydrolase superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	2,616,505	0	C	G	100.0%	49.0 / NA	14	A45G (GCC→GGC)	h16_A2404	predicted esterase of the alpha‑beta hydrolase superfamily
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (6/8);  total (6/8)

TCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGCCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTGGCGGCGCTTTACGCCAGCGGAC  >  AM260479/2616383‑2616633                                                                                                                           |                                                                                                                                 tCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGgcggcgcgg                                                                                                                       <  2:1022269/134‑1 (MQ=255)      tCCCCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGC‑CGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGgcgcggcgcgc                                                                                                                  <  2:648431/133‑1 (MQ=255)       ccTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGgcgcggcgcgc                                                                                                                  <  1:220610/133‑1 (MQ=255)       ccTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGgcgcggcgcgc                                                                                                                  <  2:1064590/133‑1 (MQ=255)                                           ctCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGaa                                                                              <  2:802376/133‑1 (MQ=255)                                            tCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCCCCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAgc                                                                            >  2:592791/1‑134 (MQ=255)                                                                  cgccgcTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTg                                                      >  2:408050/1‑134 (MQ=255)                                                                         cGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCg                                               >  2:501459/1‑134 (MQ=255)                                                                          gCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCg                                               <  1:408050/133‑1 (MQ=255)                                                                                accCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAg                                                                       <  1:1116013/103‑1 (MQ=255)                                                                                accCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAg                                                                       >  2:1116013/1‑103 (MQ=255)                                                                                              gcgGCCCGTCAAGATCGGCCTGGCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCg                          >  2:307045/1‑134 (MQ=255)                                                                                                                   ggCGCTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTGGCGGCGCTTTACGCCAGCg     >  1:1060017/1‑134 (MQ=255)                                                                                                                      gcTGGGCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTGGCGGCGCTTTACGCCAGCGGAc  <  1:424002/134‑1 (MQ=255)                                                                                                                           |                                                                                                                                 TCATTTCCTCGGTATCTCCGCGGCCGCCCTGCTGGCGGGCTGCTCGTTCGGCCCGCGTCCCGCGCCGCCGCTCGCCACCACCCCGCCCGCGCCGCGGCCCGTCAAGATCGGCCTGGCGCTGGCCGGCGGCGCGGCGCGCGGCTTTGCCCATATCGGCGTGATCAAGGCGCTCGAAGCGCAAGGCATCCATGCCGACCTGGTCACCGGCACCAGCGCCGGCGCGGTCGTGGCGGCGCTTTACGCCAGCGGAC  >  AM260479/2616383‑2616633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑