Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	3,972,184	+C	intergenic (‑136/‑395)	h16_A3682 ← / → h16_A3683	putative methyltransferase/Hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	3,972,182	1	.	C	100.0%	237.6 / NA	61	intergenic (‑134/‑397)	h16_A3682/h16_A3683	putative methyltransferase/Hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (33/28);  total (33/28)

TGCCTTTCCGTGCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑CCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTGGATG  >  AM260479/3972050‑3972300                                                                                                                                      |                                                                                                                       tGCCTTTCCGTGCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑c                                                                                                                       >  2:837949/1‑134 (MQ=255)       tCCGTGCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑ccctg                                                                                                                   <  1:282319/132‑4 (MQ=255)            gCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGa                                                                                                             >  2:549384/1‑134 (MQ=255)             cTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGAt                                                                                                            <  1:732714/134‑1 (MQ=255)             cTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGAt                                                                                                            <  1:549384/134‑1 (MQ=255)                        tttGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGtat                                                                                                  <  1:837949/133‑1 (MQ=255)                                    gCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCAtt                                                                                     >  2:255732/1‑134 (MQ=255)                                                     ttCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGAc                                                                    >  1:938853/1‑134 (MQ=255)                                                                   gggATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcg                                                                        >  2:500606/1‑116 (MQ=255)                                                                   gggATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcg                                                                        <  1:500606/116‑1 (MQ=255)                                                                     gatgatACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTcccc                                                    <  1:909574/134‑1 (MQ=255)                                                                         atACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcgcg                                                                      <  2:222889/112‑1 (MQ=255)                                                                         atACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcgcg                                                                      >  1:222889/1‑112 (MQ=255)                                                                              ggTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGAt                                                                                                            <  1:249183/69‑1 (MQ=255)                                                                              ggTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGAt                                                                                                            >  2:249183/1‑69 (MQ=255)                                                                              ggTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcgcg                                                                      <  2:596097/107‑1 (MQ=255)                                                                              ggTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcgcg                                                                      >  1:596097/1‑107 (MQ=255)                                                                              ggTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGa                                           >  2:664718/1‑134 (MQ=255)                                                                                  cAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAAc                                       >  1:969732/1‑134 (MQ=255)                                                                                   aaCCGTG‑CACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGc                                                                                                               <  2:750015/60‑1 (MQ=255)                                                                                   aaCCGTG‑CACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGc                                                                                                               >  1:750015/1‑60 (MQ=255)                                                                                    aCCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCAATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAAccc                                     >  2:935087/1‑134 (MQ=255)                                                                                     ccGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAAcccc                                    >  1:168479/1‑134 (MQ=255)                                                                                       gTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGc                                  <  2:168479/134‑1 (MQ=255)                                                                                        tGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGcc                                 >  2:138284/1‑134 (MQ=255)                                                                                        tGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGcc                                 >  2:108732/1‑134 (MQ=255)                                                                                          acacGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCt                                <  2:938853/133‑1 (MQ=255)                                                                                                     tAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACg                                                                                               >  2:51040/1‑59 (MQ=255)                                                                                                     tAACACCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACg                                                                                               <  1:51040/59‑1 (MQ=255)                                                                                                        cacCCCTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAg                 <  2:969732/134‑1 (MQ=255)                                                                                                          ccccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTcc                                                      >  2:163408/1‑95 (MQ=255)                                                                                                          ccccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTcc                                                      <  1:163408/95‑1 (MQ=255)                                                                                                          ccccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAg                 >  1:909938/1‑132 (MQ=255)                                                                                                          ccccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAg                 >  1:271617/1‑132 (MQ=255)                                                                                                          ccccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAg                 <  2:909938/132‑1 (MQ=255)                                                                                                          ccccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAg                 <  2:271617/132‑1 (MQ=255)                                                                                                          ccccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCa               >  2:173561/1‑134 (MQ=255)                                                                                                          ccccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCa               >  2:757607/1‑134 (MQ=255)                                                                                                          ccccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCa               >  1:658507/1‑134 (MQ=255)                                                                                                            ccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGc                                                                                                               >  1:835130/1‑36 (MQ=255)                                                                                                            ccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGc                                                                                                               >  1:572802/1‑36 (MQ=255)                                                                                                            ccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGc                                                                                                               <  2:835130/36‑1 (MQ=255)                                                                                                            ccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGc                                                                                                               <  2:572802/36‑1 (MQ=255)                                                                                                            ccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGAt                                                                                                            <  2:812097/39‑1 (MQ=255)                                                                                                            ccTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGAt                                                                                                            >  1:812097/1‑39 (MQ=255)                                                                                                             cTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcgcg                                                                      <  2:802417/76‑1 (MQ=255)                                                                                                             cTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCcgcg                                                                      >  1:802417/1‑76 (MQ=255)                                                                                                             cTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCAcgc            >  2:667441/1‑134 (MQ=255)                                                                                                             cTTTCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCAcgc            <  1:351091/134‑1 (MQ=255)                                                                                                              tttCCGGCGACCTCACCGTATACGCCCTGGTTGCGAt                                                                                                            <  2:327189/37‑1 (MQ=255)                                                                                                              tttCCGGCGACCTCACCGTATACGCCCTGGTTGCGAt                                                                                                            >  1:327189/1‑37 (MQ=255)                                                                                                               ttCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTcccc                                                    >  2:637159/1‑92 (MQ=255)                                                                                                               ttCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTcccc                                                    <  1:637159/92‑1 (MQ=255)                                                                                                               ttCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGAt                                          <  1:550809/102‑1 (MQ=255)                                                                                                               ttCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGAt                                          >  2:550809/1‑102 (MQ=255)                                                                                                               ttCCGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCAcgcg           >  2:486654/1‑133 (MQ=255)                                                                                                                 ccGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTcccc                                                    <  2:430744/90‑1 (MQ=255)                                                                                                                 ccGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTcccc                                                    >  1:430744/1‑90 (MQ=255)                                                                                                                 ccGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGAt        >  2:637051/1‑134 (MQ=255)                                                                                                                  cGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCt                                <  1:498356/109‑1 (MQ=255)                                                                                                                  cGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCt                                >  2:498356/1‑109 (MQ=255)                                                                                                                  cGGCGACCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGAtt       <  2:658507/134‑1 (MQ=255)                                                                                                                       aCCTCACCGTATACGCCCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTGGAtg  <  1:637051/134‑1 (MQ=255)                                                                                                                                      |                                                                                                                       TGCCTTTCCGTGCTCGTCGCCTGTTTGCCTGAGTGGCCCGCTGTGCCGGCCGTTCCGGCGCGATCGGGGATGATACCGGTGCAACCGTGACACGTATACGTAACACCCCTTTCCGGCGACCTCACCGTATACG‑CCTGGTTGCGATTGACACGTATACGGCACCCCATTTCCGCCTCAGCCGCGACGCCCGTTTACGTCCCCGCGTGTGGATAACCCCGCCTCGAACTGTGGACAAGCACGCGGATTGGATG  >  AM260479/3972050‑3972300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑