| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AM260480 | 507,425 | +A | coding (27/564 nt) | h16_B0448 → | fragment of ABC‑type transport system,periplasmic component: APC family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AM260480 | 507,423 | 1 | . | A | 97.2% | 132.7 / ‑3.3 | 36 | coding (25/564 nt) | h16_B0448 | fragment of ABC‑type transport system,periplasmic component: APC family |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (17/18); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01 | |||||||||||
ATACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATG‑AA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAATA > AM260480/507313‑507550 | aTACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATcgc > 2:447937/1‑134 (MQ=255)aTACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATcg < 1:373757/133‑1 (MQ=255)aTACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATcg > 2:373757/1‑133 (MQ=255) aCGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATcgccgccg > 2:331375/1‑134 (MQ=255) gcgTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCgcgctggc > 1:868645/1‑134 (MQ=255) ttCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCgcgctggcgct > 2:936718/1‑134 (MQ=255) ccgccTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTc < 1:936718/134‑1 (MQ=255) tGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc < 1:331375/134‑1 (MQ=255) tGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc < 1:447937/134‑1 (MQ=255) aGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGcc < 1:787590/134‑1 (MQ=255) aGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGcc < 2:868645/134‑1 (MQ=255) cGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc > 1:942458/1‑110 (MQ=255) cGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc < 2:942458/110‑1 (MQ=255) cGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCg > 2:548153/1‑134 (MQ=255) cGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCg > 1:514583/1‑134 (MQ=255) gACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATcg > 1:526405/1‑55 (MQ=255) gACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATcg < 2:526405/55‑1 (MQ=255) gACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAg < 1:947052/92‑1 (MQ=255) gACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAg > 2:947052/1‑92 (MQ=255) gACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCtga > 1:278854/1‑98 (MQ=255) gACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCtga < 2:278854/98‑1 (MQ=255) gACGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGcc > 1:621766/1‑134 (MQ=255) cGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc > 1:950025/1‑83 (MQ=255) cGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc > 2:503570/1‑83 (MQ=255) cGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc < 2:950025/83‑1 (MQ=255) cGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc < 1:503570/83‑1 (MQ=255) cGGGCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGt > 1:906623/1‑134 (MQ=255) cGGGCAATGACAATTCGACGGAGGAGACATG‑AACCAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGt > 2:456449/1‑134 (MQ=255) gCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTc < 2:325712/70‑1 (MQ=255) gCAATGACAATTCGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTc > 1:325712/1‑70 (MQ=255) cGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCggg < 1:500515/133‑1 (MQ=255) cGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCggg < 1:456449/133‑1 (MQ=255) cGACGGAGGAGACATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCggg < 2:948514/133‑1 (MQ=255) agaCATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAAt < 2:514583/134‑1 (MQ=255) agaCATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAAt < 1:545275/134‑1 (MQ=255) gaCATGAAA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAATa > 2:281548/1‑134 (MQ=255) | ATACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATG‑AA‑CAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAATA > AM260480/507313‑507550 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |