Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	2,135,859	+C	intergenic (‑237/‑97)	h16_B1889 ← / → h16_B1890	transcriptional regulator, IclR‑family/APC transporter, AAT family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	2,135,857	1	.	C	100.0%	129.2 / NA	34	intergenic (‑235/‑99)	h16_B1889/h16_B1890	transcriptional regulator, IclR‑family/APC transporter, AAT family
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (16/18);  total (16/18)

AAAGAACAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTG‑CCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATGATCCAACAACCAGAGTCCGGCAACGG  >  AM260480/2135724‑2135984                                                                                                                                       |                                                                                                                                aaaGAACAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                                  >  1:922965/1‑134 (MQ=255) aaaGAACAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                                  >  2:972350/1‑134 (MQ=255) aaaGAACAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                                  >  1:905477/1‑134 (MQ=255)     aaCAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg‑ccct                                                                                                                             >  1:663070/1‑132 (MQ=255)        aaTTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg‑ccctgc                                                                                                                           >  2:49268/1‑129 (MQ=255)             ttCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCt                                                                                                                       <  1:715025/133‑1 (MQ=255)                               aGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTg                                                                                                    <  1:405950/134‑1 (MQ=255)                                           aTCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTa                                                                                        <  2:663070/134‑1 (MQ=255)                                                             aCATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCa                                                                      <  2:309847/134‑1 (MQ=255)                                                                     ggATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTa                                                              >  1:482792/1‑134 (MQ=255)                                                                        tAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTAcc                                                            >  2:701947/1‑133 (MQ=255)                                                                              aTCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCt                                                     <  1:49268/134‑1 (MQ=255)                                                                               tccctcccCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                                  <  1:245492/56‑1 (MQ=255)                                                                               tccctcccCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                                  >  2:245492/1‑56 (MQ=255)                                                                               tccctcccCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTg                                                                                                    >  2:217529/1‑86 (MQ=255)                                                                               tccctcccCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTg                                                                                                    <  1:217529/86‑1 (MQ=255)                                                                               tccctcccCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGc                                                      >  1:800962/1‑132 (MQ=255)                                                                                    ccccGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                                  <  2:833230/51‑1 (MQ=255)                                                                                    ccccGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTActg                                                                                                                                  >  1:833230/1‑51 (MQ=255)                                                                                    ccccGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAgcg                                                 <  2:268338/132‑1 (MQ=255)                                                                                    ccccGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAgcg                                                 >  1:268338/1‑132 (MQ=255)                                                                                       cGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCgcagca                                            <  1:813904/134‑1 (MQ=255)                                                                                         ggATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCt                                                     >  1:74167/1‑123 (MQ=255)                                                                                         ggATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCt                                                     <  2:74167/123‑1 (MQ=255)                                                                                                aaaTACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGaca                                   >  1:621235/1‑134 (MQ=255)                                                                                                 aaTACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCgcag                                              <  2:942440/122‑1 (MQ=255)                                                                                                 aaTACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCgcag                                              >  1:942440/1‑122 (MQ=255)                                                                                                   tACTGAACGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACacaa                                <  1:701947/134‑1 (MQ=255)                                                                                                        aaCGACCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATg                            <  2:922965/133‑1 (MQ=255)                                                                                                            aCCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCt                                                     <  2:521603/104‑1 (MQ=255)                                                                                                            aCCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCt                                                     >  1:521603/1‑104 (MQ=255)                                                                                                            aCCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAg                                         >  2:556250/1‑116 (MQ=255)                                                                                                            aCCACGGCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAg                                         <  1:556250/116‑1 (MQ=255)                                                                                                                 ggCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACacaaca                              >  1:955338/1‑122 (MQ=255)                                                                                                                 ggCCCCAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACacaaca                              <  2:955338/122‑1 (MQ=255)                                                                                                                    cccAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATGAt                          <  2:391669/123‑1 (MQ=255)                                                                                                                    cccAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATGAt                          >  1:391669/1‑123 (MQ=255)                                                                                                                      cAGGCTGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATGATCCAACAACCagag             >  1:937793/1‑134 (MQ=255)                                                                                                                           tGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCt                                                     >  2:243764/1‑89 (MQ=255)                                                                                                                           tGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCt                                                     <  1:243764/89‑1 (MQ=255)                                                                                                                           tGGCAATTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATGATCCAACAACCAGAGTCCgg        >  1:823787/1‑134 (MQ=255)                                                                                                                               aaTTACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATGATCCAACAACCAGAGTCCGGCAAc    <  2:621235/134‑1 (MQ=255)                                                                                                                                 ttACTGCCCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATGATCCAACAACCAGAGTCCGGCAACgg  >  1:249584/1‑134 (MQ=255)                                                                                                                                       |                                                                                                                                AAAGAACAATTTTTCGGAACTTCTGGTTCCAGTTTTTAAGCGATCACCGGAGACAAGTCGACATGTACGGATAGGCCATCCCTCCCCGGGATTGCAAATACTGAACGACCACGGCCCCAGGCTGGCAATTACTG‑CCTGCCATCTATACCGAAGAGAAGCCGTGGCGCCTGCCTTAAACGCTGTACTGGCTGCAACGCAGTACCGACAGCTAGCGCAGCACAGGAGACACAACATGATCCAACAACCAGAGTCCGGCAACGG  >  AM260480/2135724‑2135984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑