Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	2,843,580	+G	intergenic (‑1/+68)	pip ← / ← h16_B2516	prolyl aminopeptidase/transcriptional regulator, LysR‑family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	2,843,579	1	.	G	100.0%	156.7 / NA	41	M1M (ATG→CTG) †	pip	prolyl aminopeptidase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (19/22);  total (19/22)

CGTGCAGGAACACCGCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑GATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAAC  >  AM260480/2843447‑2843687                                                                                                                                      |                                                                                                             cgTGCAGGAACACCGCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑g                                                                                                             <  1:923936/134‑1 (MQ=255) cgTGCAGGAACACCGCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑g                                                                                                             <  2:395288/134‑1 (MQ=255) cgTGCAGGAACACCGCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑g                                                                                                             <  2:857340/134‑1 (MQ=255) cgTGCAGGAACACCGCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑g                                                                                                             <  1:369764/134‑1 (MQ=255) cgTGCAGGAACACCGCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑g                                                                                                             <  2:559186/134‑1 (MQ=255) cgTGCAGGAACACCGCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑g                                                                                                             <  2:816463/134‑1 (MQ=255)                      tGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTc                                                                                                    <  1:205647/123‑1 (MQ=255)                      tGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTc                                                                                                    >  2:205647/1‑123 (MQ=255)                         cgcCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGa                                                                                      <  1:543512/134‑1 (MQ=255)                              ggCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCcac                                                                                 <  1:150239/134‑1 (MQ=255)                                         acGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCg                                                                      <  1:632469/134‑1 (MQ=255)                                                  gtagATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAgtg                                                                             >  2:638645/1‑118 (MQ=255)                                                  gtagATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAgtg                                                                             <  1:638645/118‑1 (MQ=255)                                                           aTGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCg                                                                                         <  2:883337/97‑1 (MQ=255)                                                           aTGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCg                                                                                         >  1:883337/1‑97 (MQ=255)                                                               ccGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAgcc                                                >  1:865695/1‑134 (MQ=255)                                                                 gTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGa                                                                                      >  2:789081/1‑94 (MQ=255)                                                                 gTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGa                                                                                      <  1:789081/94‑1 (MQ=255)                                                                 gTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGa                                                                                      >  2:900260/1‑94 (MQ=255)                                                                 gTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGa                                                                                      <  1:900260/94‑1 (MQ=255)                                                                 gTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAg                                                                               >  2:825997/1‑101 (MQ=255)                                                                 gTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAg                                                                               <  1:825997/101‑1 (MQ=255)                                                                 gTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAgcccg                                              <  1:90279/134‑1 (MQ=255)                                                                 gTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAgcccg                                              >  2:175040/1‑134 (MQ=255)                                                                           tCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAAcc                                    <  1:385805/134‑1 (MQ=255)                                                                            ccAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCg                                   >  2:921458/1‑134 (MQ=255)                                                                              aGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGc                                 <  1:175040/134‑1 (MQ=255)                                                                                cGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGa                               <  1:921458/134‑1 (MQ=255)                                                                                 gTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGa                                                                                      >  1:657684/1‑78 (MQ=255)                                                                                 gTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGa                                                                                      <  2:657684/78‑1 (MQ=255)                                                                                         ttCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCt                      >  1:755224/1‑134 (MQ=255)                                                                                                      aTTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGga                                                                                              >  2:487353/1‑49 (MQ=38)                                                                                                      aTTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGga                                                                                              <  1:487353/49‑1 (MQ=38)                                                                                                      aTTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGa                                                                                      <  2:219515/57‑1 (MQ=255)                                                                                                      aTTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGa                                                                                      >  1:219515/1‑57 (MQ=255)                                                                                                      aTTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCg                         >  2:554059/1‑118 (MQ=255)                                                                                                      aTTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCg                         <  1:554059/118‑1 (MQ=255)                                                                                                      aTTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCtt                     <  2:266787/122‑1 (MQ=255)                                                                                                      aTTTCGGGATAGAGGGTGCGCAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCtt                     >  1:266787/1‑122 (MQ=255)                                                                                                                          cAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGa                                                                                      <  2:440389/37‑1 (MQ=37)                                                                                                                          cAAGGCCGGCATGGATATGGGTCTACGGAGATCGGGa                                                                                      >  1:440389/1‑37 (MQ=37)                                                                                                                           aaGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAacac                           >  2:581481/1‑95 (MQ=255)                                                                                                                           aaGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAacac                           <  1:581481/95‑1 (MQ=255)                                                                                                                            aGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCAcc                 <  2:811486/104‑1 (MQ=255)                                                                                                                            aGGCCGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCAcc                 >  1:811486/1‑104 (MQ=255)                                                                                                                                cGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAAc  >  1:251526/1‑115 (MQ=255)                                                                                                                                cGGCATGGATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAAc  <  2:251526/115‑1 (MQ=255)                                                                                                                                      |                                                                                                             CGTGCAGGAACACCGCCGGCTTGGCGCCGGGCGTGCCCACACGCTCGTAGTAGATGACATGCCCGTCGCCGACATCCAGCGTGCCGGTTTCATAGGGCTCGATTTCGGGATAGAGGGTGCGCAAGGCCGGCAT‑GATATGGGTCTACGGAGATCGGGAGCCACAGTGTACGCCGGAGGGAACGTCAAACAGTAGCCCGCCCCTTAACCGGCGAACACCGCCTTCACCCAGTCCCTCAGCAAC  >  AM260480/2843447‑2843687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑