Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	275,969	+C	coding (268/558 nt)	h16_A0264 ←	Hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	275,968	1	.	C	100.0%	137.4 / NA	36	A90G (GCC→GGC)	h16_A0264	Hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (17/19);  total (17/19)

CCGCCGGGTGGAGTTGCGCAAGCGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGG‑CTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAGCGCGGTGCGATCCTCGCCA  >  AM260479/275837‑276091                                                                                                                                     |                                                                                                                            ccgccgGGTGGAGTTGCGCAAGCGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGG‑cc                                                                                                                           >  2:638154/1‑133 (MQ=255)      gggTGGAGTTGCGCAAGCGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTgcgc                                                                                                                       <  2:838520/134‑1 (MQ=255)             gTTGCGCAAGCGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGAtt                                                                                                                <  2:404527/134‑1 (MQ=255)                     aGCGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGAt                                                                                                        >  2:152878/1‑134 (MQ=255)                       cGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCg                                                                                                      <  2:223568/134‑1 (MQ=255)                       cGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCg                                                                                                      <  2:575383/134‑1 (MQ=255)                                             aGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGAtt                                                                                                                <  1:106159/102‑1 (MQ=255)                                             aGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGAtt                                                                                                                >  2:106159/1‑102 (MQ=255)                                             aGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGAtt                                                                                                                <  1:785916/102‑1 (MQ=255)                                             aGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGAtt                                                                                                                >  2:785916/1‑102 (MQ=255)                                             aGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGAt                                                                                                        <  1:246626/110‑1 (MQ=255)                                             aGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGAt                                                                                                        >  2:246626/1‑110 (MQ=255)                                                   gCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGAtt                                                                                                                >  2:740413/1‑96 (MQ=255)                                                   gCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGAtt                                                                                                                <  1:740413/96‑1 (MQ=255)                                                   gCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCgcag                                                                            >  1:275969/1‑132 (MQ=255)                                                        cACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCcgg                                                                     <  1:423891/134‑1 (MQ=255)                                                                     aGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCca                                                        <  1:525508/134‑1 (MQ=255)                                                                       cgcCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCcgg                                                                     <  2:885590/119‑1 (MQ=255)                                                                       cgcCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCcgg                                                                     >  1:885590/1‑119 (MQ=255)                                                                                       tCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCgtggt                                        <  1:152878/132‑1 (MQ=255)                                                                                        ccTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCca                                                        <  1:626717/115‑1 (MQ=255)                                                                                        ccTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCca                                                        >  2:626717/1‑115 (MQ=255)                                                                                            gCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAgg                                 >  1:735318/1‑134 (MQ=255)                                                                                            gCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAgg                                 >  1:873031/1‑134 (MQ=255)                                                                                             ccTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAgg                                 <  2:275969/133‑1 (MQ=255)                                                                                                tCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCAc                             >  2:328984/1‑134 (MQ=255)                                                                                                      cGGCTGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAAt                       <  2:735318/134‑1 (MQ=255)                                                                                                          tGTAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAgcg                   >  2:377642/1‑134 (MQ=255)                                                                                                            tAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGAcc                                                                                                 >  2:465045/1‑54 (MQ=39)                                                                                                            tAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGAcc                                                                                                 <  1:465045/54‑1 (MQ=39)                                                                                                            tAGAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAGcgcg                 >  2:186887/1‑134 (MQ=255)                                                                                                              gAACGCGCCGGTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAGCGCGGt               >  2:847587/1‑134 (MQ=255)                                                                                                                       ggTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCg                                                                                                      <  2:591865/38‑1 (MQ=37)                                                                                                                       ggTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCg                                                                                                      >  1:591865/1‑38 (MQ=37)                                                                                                                       ggTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGcc                               <  2:583569/109‑1 (MQ=255)                                                                                                                       ggTCGTGACGCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGcc                               >  1:583569/1‑109 (MQ=255)                                                                                                                             gaagCCGGCCTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAGCGCGGTGCGATCCTCGCCa  <  2:952709/129‑1 (MQ=255)                                                                                                                                     |                                                                                                                            CCGCCGGGTGGAGTTGCGCAAGCGGCTCGGCCATGCCCTTGCGCAGCGTGGCCAGCACCAGGGCTTGCAGCGCCCTGAAATAGCTATCCTTGCCTTCGAAGCGGCTGTAGAACGCGCCGGTCGTGACGCCGG‑CTGCGCGCAGATTTCCTGGATCGAGACCTGTTCCAGCGTGCGCTCGCAGCAGCCGGCGGCCGGCTTCCAGCAGCAGCAGCGTGGTGCGCAGGCCACGCGAATAGCGCGGTGCGATCCTCGCCA  >  AM260479/275837‑276091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑