Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	3,301,576	+G	intergenic (+45/‑146)	lspA → / → dfp	lipoprotein signal peptidase II/phosphopantothenoylcysteine synthetase/decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	3,301,574	1	.	G	100.0%	13.4 / NA	5	intergenic (+43/‑148)	lspA/dfp	lipoprotein signal peptidase II/phosphopantothenoylcysteine synthetase/decarboxylase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (1/4);  total (1/4)

GGCCTTCAACGTGGCCGACTGCGCGATCACGGTCGGCGCGGTGCTGCTGATCGTGGACGAGCTGCGCAGGGTGCGGCGCCACTGAGCGCCCTGATTGTGTTCCGCTCTCCCCTCACGGGGAGAGGCCA‑GGTGAGGGGCGGTTCAGCAAGGCACCACGCCACCAGAACCGCCCG  >  AM260479/3301447‑3301619                                                                                                                                 |                                              ggccTTCAACGTGGCCGACTGCGCGATCACGGTCGGCGCGGTGCTGCTGATCGTGGACGAGCTGCGCAGGGTGCGGCGCCACTGAGCGCCCTGATTGTGTTCCGCTCTCCCCTCACGGGGAGAGGCCA‑gggtga                                         <  1:207505/134‑5 (MQ=255) ggccTTCAACGTGGCCGACTGCGCGATCACGGTCGGCGCGGTGCTGCTGATCGTGGACGAGCTGCGCAGGGTGCGGCGCCACTGAGCGCCCTGATTGTGTTCCGCTCTCCCCTCACGGGGAGAGGCCA‑gggtga                                         <  2:758930/134‑5 (MQ=255) ggccTTCAACGTGGCCGACTGCGCGATCACGGTCGGCGCGGTGCTGCTGATCGTGGACGAGCTGCGCAGGGTGCGGCGCCACTGAGCGCCCTGATTGTGTTCCGCTCTCCCCTCACGGGGAGAGGCCA‑gggt                                           >  1:354192/1‑130 (MQ=255) ggccTTCAACGTGGCCGACTGCGCGATCACGGTCGGCGCGGTGCTGCTGATCGTGGACGAGCTGCGCAGGGTGCGGCGCCACTGAGCGCCCTGATTGTGTTCCGCTCTCCCCTCACGGGGAGAGGCCA‑gggt                                           >  1:581319/1‑130 (MQ=255)    cTTCAACGTGGCCGACTGCGCGATCACGGTCGGCGCGGTGCTGCTGATCGTGGACGAGCTGCGCAGGGTGCGGCGCCACTGAGCGCCCTGATTGTGTTCCGCTCTCCCCTCACGGGGAGAGGCCA‑g                                              >  1:683846/1‑126 (MQ=255)    cTTCAACGTGGCCGACTGCGCGATCACGGTCGGCGCGGTGCTGCTGATCGTGGACGAGCTGCGCAGGGTGCGGCGCCACTGAGCGCCCTGATTGTGTTCCGCTCTCCCCTCACGGGGAGAGGCCA‑g                                              <  2:683846/126‑1 (MQ=255)                      cgcgATCACGGTCGGCGCGGTGCTGCTGATCGTGGACGAGCTGCGCAGGGTGCGGCGCCACTGAGCGCCCTGATTGTGTTCCGCTCTCCCCTCACGGGGAGAGGCCAGGGTGAGGGGCGGTTCAGCAAGGca                       <  2:581319/132‑1 (MQ=255)                        cgATCACGGTCGGCGCGGTGCTGCTGATCGTGGACGAGCTGCGCAGGGTGCGGCGCCACTGAGCGCCCTGATTGTGTTCCGCTCTCCCCTCACGGGGAGAGGCCAGGGTGAGGGGCGGTTCAGCAAGGcaccac                   <  2:119290/134‑1 (MQ=255)                                gTCGGCGCGGTGCTGCTGATCGTGGACGAGCTGCGCAGGGTGCGGCGCCACTGAGCGCCCTGATTGTGTTCCGCTCTCCCCTCACGGGGAGAGGCCAGGGTGAGGGGCGGTTCAGCAAGGCACCACGCCACCAg           <  2:354192/134‑1 (MQ=255)                                    gcgcGGTGCTGCTGATCGTGGACGAGCTGCGCAGGGTGCGGCGCCACTGAGCGCCCTGATTGTGTTCCGCTCTCCCCTCACGGGGAGAGGCCA‑gggtga                                         >  1:606258/1‑95 (MQ=255)                                    gcgcGGTGCTGCTGATCGTGGACGAGCTGCGCAGGGTGCGGCGCCACTGAGCGCCCTGATTGTGTTCCGCTCTCCCCTCACGGGGAGAGGCCA‑gggtga                                         <  2:606258/99‑5 (MQ=255)                                                                    aGGGTGCGGCGCCACTGAGCGCCCTGATTGTGTTCCGCTCTCCCCTCACGGGGAGAGGCCa                                                <  1:636122/61‑1 (MQ=255)                                                                    aGGGTGCGGCGCCACTGAGCGCCCTGATTGTGTTCCGCTCTCCCCTCACGGGGAGAGGCCa                                                >  2:636122/1‑61 (MQ=255)                                                                                                            tcCCCTCACGGGGAGAGGCCAGGGTGAGGGGCGGTTCAGCAAGGCACCACGCCACCAGAAccgcccg  <  1:641778/67‑1 (MQ=255)                                                                                                            tcCCCTCACGGGGAGAGGCCAGGGTGAGGGGCGGTTCAGCAAGGCACCACGCCACCAGAAccgcccg  >  2:641778/1‑67 (MQ=255)                                                                                                                                 |                                              GGCCTTCAACGTGGCCGACTGCGCGATCACGGTCGGCGCGGTGCTGCTGATCGTGGACGAGCTGCGCAGGGTGCGGCGCCACTGAGCGCCCTGATTGTGTTCCGCTCTCCCCTCACGGGGAGAGGCCA‑GGTGAGGGGCGGTTCAGCAAGGCACCACGCCACCAGAACCGCCCG  >  AM260479/3301447‑3301619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑