Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	160,041	G→C	G102A (GGG→GCG)	h16_A0146 →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	160,041	0	G	C	100.0%	37.2 / NA	11	G102A (GGG→GCG)	h16_A0146	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (7/4);  total (7/4)

GGGCGCGACCGAGATGGCGTCGGGCAGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGGGGTACGA‑CCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAACCCCGACGGCCTGATCCGCTGGGAGCAGGA  >  AM260479/159935‑160131                                                                                                           |                                                                                            gggCGCGACCGAGATGGCGTCGGGCAGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCg                                                                         <  1:669256/127‑1 (MQ=255) gggCGCGACCGAGATGGCGTCGGGCAGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCg                                                                         >  2:669256/1‑127 (MQ=255)                ggCGTCGGGCAGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCt                                                   >  2:735302/1‑134 (MQ=255)                             ggcggcGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGcgacga                                                                  >  1:137851/1‑106 (MQ=255)                             ggcggcGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGcgacga                                                                  <  2:137851/106‑1 (MQ=255)                                        cAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAAcccc                            >  2:569184/1‑133 (MQ=255)                                              ggCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAACCCCGACGGcc                     <  1:735302/134‑1 (MQ=255)                                               gCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAACCCCGACGGCCt                    >  1:33965/1‑134 (MQ=255)                                               gCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAACCCCGACGGCCt                    >  2:284412/1‑134 (MQ=255)                                                                                        gggCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAACCCCGACGGCCTGATCCGCTGGGAGCAGGa  >  1:718420/1‑111 (MQ=255)                                                                                        gggCGGCGTCGATGGCGTGCGCTACGACCCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAACCCCGACGGCCTGATCCGCTGGGAGCAGGa  <  2:718420/111‑1 (MQ=255)                                                                                                           |                                                                                            GGGCGCGACCGAGATGGCGTCGGGCAGTGGCGGCGAGGGCAGCGCGGCCAGGCCTGCCGCCGCTGCCGCGCCGTCCGGCGGGCCGCAGGGCGGCGTCGATGGCGTGGGGTACGA‑CCGCCGCCGTCGGCGACGATGCACTACGCCAGCTTCGTCAACGGCGTGCAGAACCCCGACGGCCTGATCCGCTGGGAGCAGGA  >  AM260479/159935‑160131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑