Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	3,983,831	C→T	D23N (GAC→AAC)	ntrX ←	response regulator containing a Fis‑type DNA‑binding domain, NtrX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	3,983,831	0	C	T	100.0%	170.2 / NA	44	D23N (GAC→AAC)	ntrX	response regulator containing a Fis‑type DNA‑binding domain, NtrX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/23);  total (21/23)

CTTGAGCAGGGTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTCGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGACGCCATTGTTGACTATGT  >  AM260479/3983703‑3983955                                                                                                                                 |                                                                                                                             cTTGAGCAGGGTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCt                                                                                                                           >  2:399546/1‑132 (MQ=255) cTTGAGCAGGGTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTg                                                                                                                          <  2:967868/133‑1 (MQ=255) cTTGAGCAGGGTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTg                                                                                                                          <  2:871008/133‑1 (MQ=255)              aCGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGagag                                                                                                            >  1:1025780/1‑134 (MQ=255)              aCGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGagag                                                                                                            >  2:108672/1‑134 (MQ=255)              aCGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGagag                                                                                                            >  2:76900/1‑134 (MQ=255)              aCGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGagag                                                                                                            >  2:485517/1‑134 (MQ=255)                   aTCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCt                                                                                                       >  1:903953/1‑134 (MQ=255)                      ggTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTcc                                                                                                     <  1:399546/133‑1 (MQ=255)                      ggTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTcc                                                                                                     <  2:274430/133‑1 (MQ=255)                       gTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCg                                                                                                   <  1:108672/134‑1 (MQ=255)                        tATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCg                                                                                                   >  1:974307/1‑133 (MQ=255)                        tATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCg                                                                                                   >  2:145985/1‑133 (MQ=255)                                aTCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCAt                                                                                          >  1:740343/1‑134 (MQ=255)                                    aGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCg                                                                                      <  2:932522/134‑1 (MQ=255)                                             gcagcaCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACt                                                                             <  1:145985/134‑1 (MQ=255)                                                gcaCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTAc                                                                           >  1:865813/1‑133 (MQ=255)                                                 caCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGa                                                                         <  2:740343/134‑1 (MQ=255)                                                 caCGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGa                                                                         <  1:986831/134‑1 (MQ=255)                                                    gAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGa                                                                      <  2:368655/134‑1 (MQ=255)                                                      ggTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATc                                                                    <  2:865813/134‑1 (MQ=255)                                                              gTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCAt                                                            <  2:1025780/134‑1 (MQ=255)                                                                      cgcgcgGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTcccc                                                    >  2:272648/1‑134 (MQ=255)                                                                      cgcgcgGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTcccc                                                    >  2:131651/1‑134 (MQ=255)                                                                          cgGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGa                                                                         <  1:145889/109‑1 (MQ=255)                                                                          cgGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGa                                                                         >  2:145889/1‑109 (MQ=255)                                                                          cgGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCctct                                                 >  2:918099/1‑133 (MQ=255)                                                                                  gcgcgcTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCa                                         <  2:113402/133‑1 (MQ=255)                                                                                   cgcgcTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCAcc                                       <  1:485517/134‑1 (MQ=255)                                                                                         tGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATa                                                           >  1:403441/1‑108 (MQ=255)                                                                                         tGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATa                                                           <  2:403441/108‑1 (MQ=255)                                                                                           cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATa                                                           >  2:603814/1‑106 (MQ=255)                                                                                           cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATa                                                           <  1:603814/106‑1 (MQ=255)                                                                                           cAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGtt                               >  1:765652/1‑134 (MQ=255)                                                                                                cgTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGa                                                                         <  2:74293/87‑1 (MQ=255)                                                                                                cgTTCTCCGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGa                                                                         >  1:74293/1‑87 (MQ=255)                                                                                                      ccGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTcc                                                                                                     >  1:509049/1‑53 (MQ=255)                                                                                                      ccGCGAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTcc                                                                                                     <  2:509049/53‑1 (MQ=255)                                                                                                       cgcgAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCg                      <  2:974307/131‑1 (MQ=255)                                                                                                        gcgAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGACg                  <  2:903953/134‑1 (MQ=255)                                                                                                          gAGTTCAACCACGTGGCCTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGACGcc                <  1:737645/134‑1 (MQ=255)                                                                                                                          ccTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGACGCCATTGTTGACTATGt  >  1:527047/1‑132 (MQ=255)                                                                                                                           cTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGa                                                                         >  2:846662/1‑60 (MQ=255)                                                                                                                           cTTCGTTGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGa                                                                         <  1:846662/60‑1 (MQ=255)                                                                                                                                 |                                                                                                                             CTTGAGCAGGGTCACGCCATCGGTATCGGGCATCCAGATATCGAGCAGCACGAGGTCGGGCGTACCCGCCGCGCGGTATTCGCGCGCTTGCAGCGCGTTCTCCGCGAGTTCAACCACGTGGCCTTCGTCGCTGAGGATCTCCGAGAGCAGCTCCCGGATTCCCATTTCGTCATCGACTACGAGGATCGTTGCCATACTTCCCCTCTTAACCCCACCGCAGCGTTCCGGGACCGGACGCCATTGTTGACTATGT  >  AM260479/3983703‑3983955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑