Predicted mutation
evidence seq id position mutation annotation gene description
RA AM260480 433,189 A→C R133R (CGA→CGC)  h16_B0381 → Acetyl‑CoA acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM260480433,1890AC100.0% 166.2 / NA 43R133R (CGA→CGC) h16_B0381Acetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (16/27);  total (16/27)

GATGTGCGGCTCGGCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGATACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGCTACTACGCAGACCTCGCCGCCGAGGCGCGCGGCATGGGCCGC  >  AM260480/433060‑433318
                                                                                                                                 |                                                                                                                                 
gATGTGCGGCTCGGCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACg                                                                                                                               >  2:247877/1‑134 (MQ=255)
gATGTGCGGCTCGGCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACg                                                                                                                               <  2:52819/134‑1 (MQ=255)
 aTGTGCGGCTCGGCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACgg                                                                                                                              <  1:880613/134‑1 (MQ=255)
    tgCGGCTCGGCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTa                                                                                                                           >  2:735748/1‑134 (MQ=255)
      cGGCTCGGCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTAcc                                                                                                                         <  2:762503/134‑1 (MQ=255)
            ggCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCtt                                                                                                                    <  1:921067/133‑1 (MQ=255)
               cATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTggg                                                                                                                 >  1:801917/1‑133 (MQ=255)
                        cGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGt                                                                                                       <  1:39530/134‑1 (MQ=255)
                        cGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGt                                                                                                       <  1:345925/134‑1 (MQ=255)
                            aTGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCtt                                                                                                                    >  1:456169/1‑117 (MQ=255)
                            aTGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCtt                                                                                                                    <  2:456169/117‑1 (MQ=255)
                            aTGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCt                                                                                                   >  2:790368/1‑134 (MQ=255)
                               cTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGa                                                                                                <  2:817051/134‑1 (MQ=255)
                                    gcATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACg                                                                                                                               >  1:346043/1‑98 (MQ=255)
                                    gcATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACg                                                                                                                               <  2:346043/98‑1 (MQ=255)
                                    gcATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACcaca                                                                                           <  2:801917/134‑1 (MQ=255)
                                              ctgctTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGc                                                                                   <  1:187489/132‑1 (MQ=255)
                                                 ctTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGAc                                                                                <  1:735748/132‑1 (MQ=255)
                                                       ggCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCt                                                                                                   <  1:951581/107‑1 (MQ=255)
                                                       ggCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCt                                                                                                   >  2:951581/1‑107 (MQ=255)
                                                        gCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCt                                                                           >  2:749790/1‑130 (MQ=255)
                                                        gCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCt                                                                           <  1:749790/130‑1 (MQ=255)
                                                            cTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCt                                                                                                                     >  1:536578/1‑84 (MQ=255)
                                                            cTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCt                                                                                                                     <  2:536578/84‑1 (MQ=255)
                                                                       cggtggcggGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGc                                                        <  2:421547/134‑1 (MQ=255)
                                                                          tggcggGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCAc                                                     <  1:247877/134‑1 (MQ=255)
                                                                            gcgggcggCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCt                                                   <  1:634784/134‑1 (MQ=255)
                                                                            gcgggcggCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCt                                                   <  1:627384/134‑1 (MQ=255)
                                                                            gcgggcggCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCt                                                   <  1:790368/134‑1 (MQ=255)
                                                                                        gAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGctacta                                       >  1:652076/1‑134 (MQ=255)
                                                                                                  ccAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCt                                                   <  2:601986/112‑1 (MQ=255)
                                                                                                  ccAACGCGCCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCt                                                   >  1:601986/1‑112 (MQ=255)
                                                                                                         gcCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATgg                                              >  2:797474/1‑110 (MQ=255)
                                                                                                         gcCGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATgg                                              <  1:797474/110‑1 (MQ=255)
                                                                                                           cGTACATCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGCTACTACGCAGACCTCGCCGCCGAg                    <  2:468330/134‑1 (MQ=255)
                                                                                                                 tCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCt                                                   >  2:375676/1‑97 (MQ=255)
                                                                                                                 tCGTCCCGCGCGGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCt                                                   <  1:375676/97‑1 (MQ=255)
                                                                                                                        gcgcgGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCt                                                                                                   <  2:388937/42‑1 (MQ=255)
                                                                                                                        gcgcgGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCt                                                                                                   >  1:388937/1‑42 (MQ=255)
                                                                                                                        gcgcgGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGCTACTACGCAGACCTCGCCGCCGAGGCGCGCGGCATgg       <  2:261783/134‑1 (MQ=255)
                                                                                                                         cgcgGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTc                                                                                                     <  1:822180/39‑1 (MQ=38)
                                                                                                                         cgcgGCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTc                                                                                                     >  2:822180/1‑39 (MQ=38)
                                                                                                                             gCCGCTACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGCTACTACGCAGACCTCGCCGCCGAGGCGCGCGGCATGGGCcgc  >  2:2374/1‑134 (MQ=255)
                                                                                                                                 |                                                                                                                                 
GATGTGCGGCTCGGCCATGAAGTCCGTGATGCTCGCGCATGACCTGCTGCTTGCCGGCACCTGCAACGTGGCGGTGGCGGGCGGCATGGAGTCGATGACCAACGCGCCGTACATCGTCCCGCGCGGCCGATACGGCTACCGCCTTGGGCACGGCCAGTTCCTCGACCACATGTACTGCGACGGGCTGGAGGACGCCTACGAAGGCCACCTGATGGGCTACTACGCAGACCTCGCCGCCGAGGCGCGCGGCATGGGCCGC  >  AM260480/433060‑433318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑