Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260480	753,133	T→C	K37E (AAG→GAG)	h16_B0675 ←	Acyl‑CoA dehydrogenase,short‑chain specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260480	753,133	0	T	C	100.0%	150.2 / NA	39	K37E (AAG→GAG)	h16_B0675	Acyl‑CoA dehydrogenase,short‑chain specific
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/21);  total (18/21)

CCAGTTCGCGCTGCACCACCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTTGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCTTCAGA  >  AM260480/753007‑753259                                                                                                                               |                                                                                                                               ccAGTTCGCGCTGCACCACCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTgcg                                                                                                                         >  2:976763/1‑134 (MQ=255) ccAGTTCGCGCTGCACCACCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTgcg                                                                                                                         <  2:94877/134‑1 (MQ=255)         cgcTGCACCACCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTgcg                                                                                                                         >  2:601398/1‑126 (MQ=255)         cgcTGCACCACCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTgcg                                                                                                                         <  1:601398/126‑1 (MQ=255)         cgcTGCACCACCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCCCCTGCGCGCGCGct                                                                                                                 >  2:267173/1‑134 (MQ=255)              caccacCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCtt                                                                                                              <  2:96761/132‑1 (MQ=255)                   ccaGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGcggcgg                                                                                                       >  2:397228/1‑134 (MQ=255)                          gctggcGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTc                                                                                                  <  1:201903/132‑1 (MQ=255)                          gctggcGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCg                                                                                                 >  2:806985/1‑133 (MQ=255)                          gctggcGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGa                                                                                                <  1:818487/134‑1 (MQ=255)                                 gggCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTa                                                                                         >  1:184841/1‑134 (MQ=255)                                    ccTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTc                                                                                      <  1:267173/134‑1 (MQ=255)                                            gAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGa                                                                              <  1:397228/134‑1 (MQ=255)                                                  gccgAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGc                                                                        <  1:910068/134‑1 (MQ=255)                                                    cgAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGa                                                                      <  1:624927/134‑1 (MQ=255)                                                      aaGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACg                                                                    >  2:432777/1‑134 (MQ=255)                                                                gcACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCtt                                                                                                              <  2:776022/82‑1 (MQ=255)                                                                gcACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCtt                                                                                                              >  1:776022/1‑82 (MQ=255)                                                                        aGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCa                                                   <  2:184841/133‑1 (MQ=255)                                                                               gCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGc                                           <  1:432777/134‑1 (MQ=255)                                                                               gCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGc                                           <  1:976763/134‑1 (MQ=255)                                                                                    cccATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTg                                                                                                                           <  1:844250/49‑1 (MQ=255)                                                                                    cccATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTc                                      >  2:978003/1‑134 (MQ=255)                                                                                    cccATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAAGCTCTCGCCTg                                                                                                                           >  2:844250/1‑49 (MQ=39)                                                                                         ttCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTg                                  >  2:273255/1‑133 (MQ=255)                                                                                             gCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGt                             >  2:21155/1‑134 (MQ=255)                                                                                                  cTTGTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGt                        <  1:273255/134‑1 (MQ=255)                                                                                                     gTTCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTcc                      <  1:21155/133‑1 (MQ=255)                                                                                                       tCCACACGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATg                   >  2:597817/1‑134 (MQ=255)                                                                                                           cacGCCGCGATCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAg               <  1:978003/134‑1 (MQ=255)                                                                                                                    aTCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACg                                                                                             >  2:696185/1‑47 (MQ=255)                                                                                                                    aTCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACg                                                                                             <  1:696185/47‑1 (MQ=255)                                                                                                                    aTCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTa                                <  1:946158/108‑1 (MQ=255)                                                                                                                    aTCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTa                                >  2:946158/1‑108 (MQ=255)                                                                                                                    aTCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAg                         <  2:38244/115‑1 (MQ=255)                                                                                                                    aTCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAg                         >  1:38244/1‑115 (MQ=255)                                                                                                                     tCCAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCtt      >  2:421471/1‑133 (MQ=255)                                                                                                                      ccAGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCtt      >  1:731555/1‑132 (MQ=255)                                                                                                                        aGGCTCTCGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCTTCAGa  <  1:597817/134‑1 (MQ=255)                                                                                                                               |                                                                                                                               CCAGTTCGCGCTGCACCACCAGCTGGCTGGCGGGGCCTTCGCCGAAGCCGCCGAAGTCGGCCGGCACGGTCAGGCCCAGCACGCCCATTTCAGCGAGCTTGTTCCACACGCCGCGATCCAGGCTCTTGCCTGCGCGCGCGCTCTTGCGGCGGGCCTCGAACGTGTATTCGGTATCGATGAAGCGACGCAGGCTGTCCGCCAGCATCTGTTGCTCTTCGCTGTAGGTGAAGTCCATGTCAGCTATCCCTTCAGA  >  AM260480/753007‑753259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑