Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	208,932	A→C	H322Q (CAT→CAG)	h16_A0195 ←	Putative GTPase (G3E family)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	208,932	0	A	C	100.0%	150.3 / NA	39	H322Q (CAT→CAG)	h16_A0195	Putative GTPase (G3E family)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/24);  total (15/24)

CAGGCAGTTCTCCAGGCCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCATGGAACACCACTTTGCGGTCAACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAAT  >  AM260479/208803‑209028                                                                                                                                  |                                                                                                 caggcAGTTCTCCAGGCCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTgg                                                                                               >  1:934294/1‑133 (MQ=255)     cAGTTCTCCAGGCCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAacac                                                                                          <  1:244031/134‑1 (MQ=255)     cAGTTCTCCAGGCCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGCCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAacac                                                                                          <  2:934294/134‑1 (MQ=255)        ttCTCCAGGCCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAAcaccac                                                                                       >  1:961801/1‑134 (MQ=255)                 cccTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGt                                                                               <  2:886289/133‑1 (MQ=255)                 cccTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGt                                                                               >  1:886289/1‑133 (MQ=255)                 cccTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTc                                                                              <  1:370439/134‑1 (MQ=255)                  ccTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                                             <  2:546706/134‑2 (MQ=255)                  ccTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                                             <  2:407245/134‑2 (MQ=255)                      gAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTccac                                                                           <  1:533494/132‑4 (MQ=255)                      gAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCacac                                                                         >  2:131555/1‑134 (MQ=255)                      gAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGCTGGACCCCCTGGAACACCACTTTGCGGTccac                                                                           <  2:974265/132‑4 (MQ=255)                       aGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc                                                                        <  2:499190/134‑1 (MQ=255)                       aGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACAcc                                                                        <  2:1010167/134‑1 (MQ=255)                         gATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAAcacca                                                                                        <  2:504361/116‑1 (MQ=255)                         gATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAAcacca                                                                                        >  1:504361/1‑116 (MQ=255)                         gATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                                             >  1:814566/1‑126 (MQ=255)                         gATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                                             <  2:814566/127‑2 (MQ=255)                          aTGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTc                                                                     <  1:131555/134‑1 (MQ=255)                          aTGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTc                                                                     <  2:961801/134‑1 (MQ=255)                          aTGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTc                                                                     <  2:477769/134‑1 (MQ=255)                                                    gATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                                             >  2:403936/1‑99 (MQ=255)                                                    gATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                                             <  1:756822/100‑2 (MQ=255)                                                    gATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                                             >  2:756822/1‑99 (MQ=255)                                                    gATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                                             <  1:403936/100‑2 (MQ=255)                                                                   cgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                                             >  1:640610/1‑84 (MQ=255)                                                                   cgTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                                             <  2:640610/85‑2 (MQ=255)                                                                         cGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACgg                      >  2:922974/1‑134 (MQ=255)                                                                           gggTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGcc                                                      <  2:908816/100‑1 (MQ=255)                                                                           gggTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGcc                                                      >  1:908816/1‑100 (MQ=255)                                                                                  ttCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                                             >  2:386880/1‑69 (MQ=255)                                                                                  ttCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                                             <  1:386880/70‑2 (MQ=255)                                                                                      tccCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                                             <  1:731453/66‑2 (MQ=255)                                                                                      tccCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTcc                                                                             >  2:731453/1‑65 (MQ=255)                                                                                              ggCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAAt  <  1:922974/133‑1 (MQ=255)                                                                                                  ccGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGttt                                    <  2:206173/95‑1 (MQ=255)                                                                                                  ccGATGTCGCTGCCCATCAGCTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGttt                                    >  1:206173/1‑95 (MQ=255)                                                                                                                      cTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACa       <  1:93994/104‑1 (MQ=255)                                                                                                                      cTGGTGGACCCCCTGGAACACCACTTTGCGGTCCACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACa       >  2:93994/1‑104 (MQ=255)                                                                                                                                  |                                                                                                 CAGGCAGTTCTCCAGGCCCTTGAGGATGGCGTCGCGGGGCAGGTCCACGCCGATGAACACCATGCGCGTGCCCGGGGTCTCTTCCTCCCACTTGGCGCCGATGTCGCTGCCCATCAGCTGGTGGACCCCATGGAACACCACTTTGCGGTCAACACCGTCCATATATAGTACGCCCTTGTAGCGCAGCAGTTTTTCCCCGTATACGGCCAGGATGCCGGACAGGAAT  >  AM260479/208803‑209028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑