Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	1,402,540	C→G	G893A (GGC→GCC)	h16_A1292 ←	ATP‑dependent transcriptional regulator,MalT‑family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	1,402,540	0	C	G	100.0%	52.3 / NA	15	G893A (GGC→GCC)	h16_A1292	ATP‑dependent transcriptional regulator,MalT‑family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (9/6);  total (9/6)

CGGTCGCGGACGCGCGCCACGGCCTCGTCGCGGCTGCTCACGCCCAGCTTGCGGAAGATGTTGCGCAAGTGCCATTTCACCGTCTCCGGCGACAGGCCCAGGGTGCGCGCAATCTTCTTGTTGGGCAGGCCCTGGCCGAGCAGGCGCACGACATCGGCTTCGCGGTCGCTCAGTTCCTCGAGGCGCGCGTCGGCCCGGGCCGGGCTGCCGGCGGGCGCGGCAGCCGCGGCGGTGCCGCCCGCC  >  AM260479/1402411‑1402653                                                                                                                                  |                                                                                                                  cggtcgcggACGCGCGCCACGGCCTCGTCGCGGCTGCTCACGCCCAGCTTGCGGAAGATGTTGCGCAAGTGCCATTTCACCGTCTCCGGCGACAGGCCCAGGGTGCGCGCAATCTTCTTGTTGGGCAGGGCCTg                                                                                                               >  1:786691/1‑134 (MQ=255)                gcCACGGCCTCGTCGCGGCTGCTCACGCCCAGCTTGCGGAAGATGTTGCGCAAGTGCCATTTCACCGTCTCCGGCGACAGGCCCAGGGTGCGCGCAATCTTCTTGTTGGGCAGGGCCTGGCCGAGCAGGCGCAc                                                                                                <  2:786691/134‑1 (MQ=255)                  cACGGCCTCGTCGCGGCTGCTCACGCCCAGCTTGCGGAAGATGTTGCGCAAGTGCCATTTCACCGTCTCCGGCGACAGGCCCAGGGTGCGCGCAATCTTCTTGTTGGGCAGGGCCTGGCCGAGCAGGCGCACGa                                                                                              >  1:574705/1‑134 (MQ=255)                           gtcgCGGCTGCTCACGCCCAGCTTGCGGAAGATGTTGCGCAAGTGCCATTTCACCGTCTCCGGCGACAGGCCCAGGGTGCGCGCAATCTTCTTGTTGGGCAGGGCCTGGCCGAGCAGGCGCACGACATCGGc                                                                                       <  2:574705/132‑1 (MQ=255)                                               gCTTGCGGAAGATGTTGCGCAAGTGCCATTTCACCGTCTCCGGCGACAGGCCCAGGGTGCGCGCAATCTTCTTGTTGGGCAGGGCCTGGCCGAGCAGGCGCACGACATCGGCTTCGCGGTCGCTCAGTTCCTCg                                                                 <  2:748537/134‑1 (MQ=255)                                                                        ccATTTCACCGTCTCCGGCGACAGGCCCAGGGTGCGCGCAATCTTCTTGTTGGGCAGGGCCTGGCCGAGCAGGCGCACGACATCGGCTTCGCGGTCGCTCAGTTCCTCGAGGCGCGCGTCGGCccgggccggg                                         <  2:444689/133‑1 (MQ=255)                                                                         cATTTCACCGTCTCCGGCGACAGGCCCAGGGTGCGCGCAATCTTCTTGTTGGGCAGGGCCTGGCCGAGCAGGCGCACGACATCGGCTTCGCGGTCGCTCAGTTCCTCGAGGCGCGCGTCGGCCCGGGCCGGGCt                                       >  1:494104/1‑134 (MQ=255)                                                                                     tcCGGCGACAGGCCCAGGGTGCGCGCAATCTTCTTGTTGGGCAGGGCCTGGCCGAGCAGGCGCACGACATCGGCTTCGCGGt                                                                               >  1:689739/1‑82 (MQ=255)                                                                                     tcCGGCGACAGGCCCAGGGTGCGCGCAATCTTCTTGTTGGGCAGGGCCTGGCCGAGCAGGCGCACGACATCGGCTTCGCGGt                                                                               <  2:689739/82‑1 (MQ=255)                                                                                          cGACAGGCCCAGGGTGCGCGCAATCTTCTTGTTGGGCAGGGCCTGGCCGAGCAGGCGCACGACATCGGCTTCGCGGTCGCTCAGTTCCTCGAGGCGCGCGTCGGCCCGGGCCGGGCTGCCGGCGGGCGCGGCAg                      >  1:643561/1‑134 (MQ=255)                                                                                                              cAATCTTCTTGTTGGGCAGGGCCTGGCCGAGCAGGCGCACGACATCGGCTTCGCGGTCGCTCAGTTCCTCGAgg                                                              <  1:3050/74‑1 (MQ=255)                                                                                                              cAATCTTCTTGTTGGGCAGGGCCTGGCCGAGCAGGCGCACGACATCGGCTTCGCGGTCGCTCAGTTCCTCGAgg                                                              >  2:3050/1‑74 (MQ=255)                                                                                                              cAATCTTCTTGTTGGGCAGGGCCTGGCCGAGCAGGCGCACGACATCGGCTTCGCGGTCGCTCAGTTCCTCGAGGCGCGCGTCGGCCCGGGCCGGGCTGCCGGCGGGCGCGGCAGCCGCGGCGGTGCcgcccgcc  >  1:352615/1‑134 (MQ=255)                                                                                                              cAATCTTCTTGTTGGGCAGGGCCTGGCCGAGCAGGCGCACGACATCGGCTTCGCGGTCGCTCAGTTCCTCGAGGCGCGCGTCGGCCCGGGCCGGGCTGCCGGCGGGCGCGGCAGCCGCGGCGGTGCcgcccgcc  >  1:533334/1‑134 (MQ=255)                                                                                                              cAATCTTCTTGTTGGGCAGGGCCTGGCCGAGCAGGCGCACGACATCGGCTTCGCGGTCGCTCAGTTCCTCGAGGCGCGCGTCGGCCCGGGCCGGGCTGCCGGCGGGCGCGGCAGCCGCGGCGGTGCcgcccgcc  >  2:525647/1‑134 (MQ=255)                                                                                                                                  |                                                                                                                  CGGTCGCGGACGCGCGCCACGGCCTCGTCGCGGCTGCTCACGCCCAGCTTGCGGAAGATGTTGCGCAAGTGCCATTTCACCGTCTCCGGCGACAGGCCCAGGGTGCGCGCAATCTTCTTGTTGGGCAGGCCCTGGCCGAGCAGGCGCACGACATCGGCTTCGCGGTCGCTCAGTTCCTCGAGGCGCGCGTCGGCCCGGGCCGGGCTGCCGGCGGGCGCGGCAGCCGCGGCGGTGCCGCCCGCC  >  AM260479/1402411‑1402653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑