BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM260479	342,407	T→C	L180L (TTG→CTG)	amtB →	ammonium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM260479	342,407	0	T	C	100.0%	163.1 / NA	42	L180L (TTG→CTG)	amtB	ammonium transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/20); total (22/20)

TGGCTACAGCTTTGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAATTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCACCTTCTCC  >  AM260479/342283‑342532

tGGCTACAGCTTTGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTgg                                                                                                                            <  1:998377/128‑1 (MQ=255)

tGGCTACAGCTTTGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGc                                                                                                                          <  1:717821/130‑1 (MQ=255)

tGGCTACAGCTTTGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGc                                                                                                                          >  2:717821/1‑130 (MQ=255)

          tttGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTc                                                                                                              >  2:100683/1‑132 (MQ=255)

          tttGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTc                                                                                                              >  2:653436/1‑132 (MQ=255)

          tttGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTc                                                                                                              >  2:742438/1‑132 (MQ=255)

                ttCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTgcgc                                                                                                      >  2:686445/1‑134 (MQ=255)

                  cACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGcc                                                                                                     <  1:653436/133‑1 (MQ=255)

                        gggCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCt                                                                                              <  2:173594/134‑1 (MQ=255)

                            aaCGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGc                                                                                                                          >  1:406515/1‑102 (MQ=255)

                            aaCGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGc                                                                                                                          <  2:406515/102‑1 (MQ=255)

                            aaCGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCAt                                                                                          >  1:537498/1‑134 (MQ=255)

                                 cttcttCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCt                                                                                     >  2:309156/1‑134 (MQ=255)

                                  ttcttcGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTg                                                                                    >  1:546896/1‑134 (MQ=255)

                                                 gaccgGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCt                                                                                              <  1:984243/109‑1 (MQ=255)

                                                 gaccgGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCt                                                                                              >  2:984243/1‑109 (MQ=255)

                                                     ggCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGcc                                                                 <  1:964932/134‑1 (MQ=255)

                                                      gCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCt                                                                >  2:274037/1‑134 (MQ=255)

                                                                aaGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAAcg                                                      <  2:537498/134‑1 (MQ=255)

                                                                    ggCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACgcgc                                                   >  1:101930/1‑133 (MQ=255)

                                                                     gCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGcc                                                  >  2:929932/1‑133 (MQ=255)

                                                                     gCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCa                                                 <  1:686445/134‑1 (MQ=255)

                                                                        gACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGt                                              >  2:772341/1‑134 (MQ=255)

                                                                         aCGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAg                                               <  2:101930/132‑1 (MQ=255)

                                                                         aCGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACCGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAAcg                                                      <  2:605343/125‑1 (MQ=255)

                                                                         aCGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACCGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAAcg                                                      >  1:605343/1‑125 (MQ=255)

                                                                          cGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTtc                                            <  2:546896/134‑1 (MQ=255)

                                                                            gTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGcc                                                                                                     <  1:815140/75‑1 (MQ=255)

                                                                            gTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGcc                                                                                                     >  2:815140/1‑75 (MQ=255)

                                                                            gTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTtctc                                          >  2:278042/1‑134 (MQ=255)

                                                                                           gccACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTc                                                              <  1:866144/99‑1 (MQ=255)

                                                                                           gccACCTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTc                                                              >  2:866144/1‑99 (MQ=255)

                                                                                               ccTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCgtg                       <  1:274037/134‑1 (MQ=255)

                                                                                                cTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTc                                                                                                              >  2:524515/1‑46 (MQ=255)

                                                                                                cTTCAGCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTc                                                                                                              <  1:524515/46‑1 (MQ=255)

                                                                                                     gCAAGGGCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCtg                 <  1:929932/134‑1 (MQ=255)

                                                                                                          ggCGTGGTGGTGCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGtt            <  1:742438/134‑1 (MQ=255)

                                                                                                             gtggtggtgCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCAc         >  1:587906/1‑134 (MQ=255)

                                                                                                              tggtggtgCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTgg              <  2:150230/128‑1 (MQ=255)

                                                                                                              tggtggtgCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTgg              >  1:150230/1‑128 (MQ=255)

                                                                                                                    tgCCGGAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCACCTTCTcc  >  1:896760/1‑134 (MQ=255)

                                                                                                                         gAACTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCa          <  2:935101/121‑1 (MQ=255)

TGGCTACAGCTTTGCCTTCACCGAGGGCAACGCCTTCTTCGGCGGCACCGACCGGCTCTTCATGAAGGGGCTGACGGTCGAGGCCGTGGCCGCCACCTTCAGCAAGGGCGTGGTGGTGCCGGAATTGGGCTACTTCGCCTTCCAGTGCGCCTTCGCCTGCATCACCTGCGGCCTGATCATCGGCGCCTTCGCCGAACGCGCCAAGTTCTCGGCCGTGCTGGTGTTCGTGGTGCTGTGGTTCACCTTCTCC  >  AM260479/342283‑342532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`