| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | TrimmedAssembly_T66_contig_9 | 36 | T→C | intergenic (–/+223) | – / ← rasttk_feature_annotation_tool=elepa@bvbrc | –/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | TrimmedAssembly_T66_contig_9 | 36 | 0 | T | C | 81.7% | 810.0 / 164.1 | 320 | intergenic (–/+223) | –/rasttk_feature_annotation_tool=elepa@bvbrc | –/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (24/34); new base C (108/151); total (134/186) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.11e-01 | |||||||||||
Only 100 of 324 total aligned reads displayed.
‑‑‑‑‑‑GATAATTTCGTAGAGTATCGGGTGCGACCGATGC‑TTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGCAATTGCTAGTTGACCTT > TrimmedAssembly_T66_contig_9/1‑171 | ttcgaggataattTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctc > 2:325363‑M2/7‑84 (MQ=255) tcgaggataatTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcg < 2:2253687‑M2/131‑61 (MQ=255) cgaggATAATTTCGTAGAGTATCGGGTGCGACCGATGC‑TTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCctct < 2:121679/132‑1 (MQ=255) aggatAATTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAATCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgt < 2:2412082‑M2/134‑4 (MQ=255) aggatAATTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctctttt < 1:1920559‑M2/134‑57 (MQ=255) aggatAATTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctctttt < 2:1489822‑M2/134‑57 (MQ=255) aggatAATTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcct < 2:168106‑M2/134‑57 (MQ=255) aggatAATTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAGATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgt < 2:351482‑M2/134‑4 (MQ=255) aggatAATTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgt < 1:2435718‑M2/134‑4 (MQ=255) ggaTAATTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgttt < 1:2213463‑M2/107‑37 (MQ=255) aTAATTTCGTAGAGTATCGGGTGCGACCGATGC‑ATTTTTTTTTATTGTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCg < 1:1515344/134‑1 (MQ=255) aTAATTTCGTAGAGTATCGGGTGCGACCGATGC‑‑TCTTTTTTTATTTTCt < 2:1190478/49‑1 (MQ=255) aTAATTTCGTAGAGTATCGGGTGCGACCGATGC‑‑TCTTTTTTTATTTTCt > 1:1190422/1‑49 (MQ=255) tAATTTCGTAGAGTATCGGGTGCGACCGATGCTTTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTa < 2:1457270/136‑1 (MQ=255) tAATTTCGTAGAGTATCGGGTGCGACCGATGC‑TTTTTTTTTTATTTTATATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTa < 1:2171115/135‑1 (MQ=255) tAATTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttt > 2:1117968‑M2/1‑76 (MQ=255) tAATTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttt > 1:1655637‑M2/1‑76 (MQ=255) tAATTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcttttttg > 1:2323927‑M2/1‑69 (MQ=255) tAATTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgttttt > 2:395196‑M2/1‑129 (MQ=255) tAATTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgttttt > 1:1968308‑M2/1‑129 (MQ=255) tAATTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTGTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttt > 2:550051‑M2/1‑76 (MQ=255) aaTTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAATAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgttt < 2:1094310‑M2/104‑37 (MQ=255) aaTTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAACAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgttt > 1:1094310‑M2/1‑68 (MQ=255) aaTTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttata > 2:2002222‑M2/1‑75 (MQ=255) aaTTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgtatgggtt < 2:608234‑M2/136‑62 (MQ=255) aaTTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctct > 1:434494‑M2/1‑68 (MQ=255) aaTTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaa < 1:406924‑M2/80‑13 (MQ=255) aaGTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgttttttta < 2:220730‑M2/136‑69 (MQ=255) tttCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAATGCAGGTCTGGAAAAATtcgaagatttttccagacctgcgttgtttctagagacttatgtcacagcctcgtatgggttaa < 2:702326‑M2/136‑64 (MQ=255) tttCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcttttttgttct < 2:668702‑M2/136‑71 (MQ=255) tttCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATAAAAAGCAGGTCTTGAAAAATtcgaagatttttccagacctgctttgtttctagagacatatgtcacagccacgtaagggttaa < 1:1582287‑M2/136‑64 (MQ=255) ttCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcttttttgttcta > 1:1832354‑M2/1‑65 (MQ=255) ttCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcttttttgttcta > 1:1807453‑M2/1‑65 (MQ=255) ttCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcttttttgttcta > 2:1832833‑M2/1‑65 (MQ=255) cGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgtttttgatgtc > 1:1445974‑M2/1‑123 (MQ=255) gTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgtatgggttaatcga > 1:25130‑M2/1‑69 (MQ=255) agTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgtatgggttaatcga < 2:1743002‑M2/132‑68 (MQ=255) agTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttttgaaagtgttag < 2:42435‑M2/136‑72 (MQ=255) agTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgtttttgatgtctatta < 2:2400692‑M2/136‑19 (MQ=255) gTATCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgtttttgatgtctatt > 1:1109596‑M2/1‑117 (MQ=255) aTCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttttgaaagtgttagtt > 1:1460728‑M2/1‑62 (MQ=255) aTCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttttgaaagtgttagtt > 1:1327073‑M2/1‑62 (MQ=255) aTCGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattactcatcgtta < 1:611975‑M2/136‑82 (MQ=255) aTCGGGTGCGACCGATGC‑TCTTTTTATAAGGT‑CTATTATATCAAAAACAGGACTGGAAAAATCCGAAGATTTTTCCAGACCTGATTTGTTTCTAGAGACTTATGTCACAGCCTCTtgtttttgatgtctattaaa > 2:909384‑M2/1‑115 (MQ=255) cGGGTGCGACCGATGC‑TTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAATTCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCGTCGGAGTTAACAACTGCATTGc > 1:1007602/1‑136 (MQ=255) cGGGTGCGACCGATGC‑TTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGc < 1:771921/136‑1 (MQ=255) cGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctagcctttttgaaagtgttagttcgt < 2:41119‑M2/136‑77 (MQ=255) cGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattactcatcgttatt < 1:675584‑M2/136‑84 (MQ=255) cGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagag < 1:1894884‑M2/95‑43 (MQ=255) cGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgttt > 1:1879649‑M2/1‑53 (MQ=255) cGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgttt < 2:1879649‑M2/89‑37 (MQ=255) cGGGTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgtttttgatgtctattaaaatg > 2:1014912‑M2/1‑113 (MQ=255) ggTGCGACCGATGC‑TTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGATTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGCaa > 1:824055/1‑136 (MQ=255) gTGCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttttgaaagtgttagttcgttg < 2:2374888‑M2/135‑79 (MQ=255) gCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAATCAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattactcatcgttattaatca < 1:468581‑M2/136‑89 (MQ=255) gCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttataaaaaatatttaaatgtctt < 2:960757‑M2/135‑81 (MQ=255) gCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgtatgggttaatcgaaaaattctttata > 2:254981‑M2/1‑55 (MQ=255) gCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttttgaaagtgttagttcgttgacg > 2:1363241‑M2/1‑55 (MQ=255) gCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttttgaaagtgttagttcgttgacg > 1:2360935‑M2/1‑55 (MQ=255) gCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattactcatcgttattaaac > 2:2160178‑M2/1‑48 (MQ=255) gCGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTATGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattactcatcgttattaaac > 2:1840720‑M2/1‑48 (MQ=255) gCGACCGATGC‑TATTTTTTT‑ATTTTCTATTATATCAAAAGCTGGTCTGGAAAAATtcgaagatttttccagacctgatttgtttctagagacttatgtcacagcctcgtatgggttaatcgaaaaattctttata > 1:214707‑M2/1‑55 (MQ=255) gCGACCGAGGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttataaaaaatatttaaatgtctt < 2:2313942‑M2/135‑81 (MQ=255) gCGACCGAAGC‑TCTTTTTTT‑ATTATCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCCAGAGACTTATGTCACAGCCTCTtgtttttgatgtctattaaaatggaccc > 2:1057040‑M2/1‑108 (MQ=255) cGACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttat > 2:2180770‑M2/1‑54 (MQ=255) gccctttGC‑TATTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATATTTCCAGACCTGCTTTGTTTCTAGAGACGTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGCAATTGCt < 2:1163230/130‑1 (MQ=38) gACCGATGC‑TTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGCAATTGCt < 2:286449/136‑1 (MQ=255) gACCGATGC‑TTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGCAATTGCt < 1:534849/136‑1 (MQ=255) gACCGATGC‑TTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGCAATTGCt < 2:2072138/136‑1 (MQ=255) gACCGATGC‑TTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCAGGAAAAATCCGAAGATTTTTCCAGACCTTCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTAGTATTTAACAAATGCATTGCAATAGCt > 1:1722953/1‑136 (MQ=37) gACCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgtatgggttaatcgaaaaattctttatata < 1:1747419‑M2/135‑83 (MQ=255) caCCGATGC‑ATTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAACATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGCAATTGCt < 1:2027699/135‑1 (MQ=255) aCCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttaaaaaaaatatttaaatgtcttgaaa < 2:2093632‑M2/136‑85 (MQ=255) aCCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgtatgggttaatcgaaaaattctttatataac < 1:1254049‑M2/136‑85 (MQ=255) aCCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttcca < 1:875586‑M2/67‑16 (MQ=255) aCCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttttgaaagtgttagttcgttgacgttt < 2:1479652‑M2/136‑85 (MQ=255) aCCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttttgaaagtgttagttcgttgacgttt < 2:1263407‑M2/136‑85 (MQ=255) aCCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattactcatcgttattaaacaatc < 1:935874‑M2/136‑92 (MQ=255) aCCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgtttttgatgtctattaaaatggacccta < 2:76534‑M2/135‑31 (MQ=255) aCCGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgtttttgatgtctattaaaatggacccta < 1:562465‑M2/135‑31 (MQ=255) ccGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttataaaaaatatttaaatgtcttgcaaa < 1:2271204‑M2/136‑86 (MQ=255) ccGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttttgaaagtgttagttcgttgacgtttg > 1:2415270‑M2/1‑51 (MQ=255) ccGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttttgaaagtgttagttcgt < 2:1245069‑M2/127‑77 (MQ=255) ccGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtc < 2:1865328‑M2/95‑52 (MQ=255) ccGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTAAAGTCACAGCCTCTtgttattgatgtatataaaaatggaccctacc < 1:271528‑M2/136‑33 (MQ=255) ccGATGC‑TCTTTTTTT‑ATTTTCGATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgtttttgatgtctattaaaatggaccctacc < 1:1613955‑M2/136‑33 (MQ=255) cGATGC‑TTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGCAATTGCTAGt > 2:487047/1‑136 (MQ=255) cGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAATACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTGTTGTTTCTAGAGACTTATGTCACAGCCTCTtgtttttgatgtctattaaaatggaccctaccg < 2:1943109‑M2/136‑34 (MQ=255) cGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgtagatttttccagacctgctttgttgctagagacttatgtcacagcctcttttttataaaaaatatttaaatgtcttgcaaaa < 1:1475926‑M2/136‑87 (MQ=255) cGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgtatgggttaatcgaaaaattctttatataacgc < 2:161515‑M2/136‑87 (MQ=255) cGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgtatgggttaatcgaaaaattctttatataacgc < 2:440621‑M2/136‑87 (MQ=255) cGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttttgaaagtgttagttcgttgacgtttga < 1:1670721‑M2/136‑87 (MQ=255) cGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttttgaaagtgttagttcgttgacgtttga > 1:1244415‑M2/1‑50 (MQ=255) cGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttttgaaagtgttagttcgttgacgtttga < 2:867402‑M2/136‑87 (MQ=255) cGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattactcatcgttattaaacaatcat < 1:1352575‑M2/136‑94 (MQ=255) cGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattactcatcgttattaaacaatcat < 1:211768‑M2/136‑94 (MQ=255) cGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgtttttgatgtctattaaaatggaccctaccg < 2:1778884‑M2/136‑34 (MQ=255) cGATGC‑TCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgtttttgatgtctattaaaatggaccctaccg < 1:363236‑M2/136‑34 (MQ=255) gATGC‑TTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGCAATTGCTAGtt > 1:2078081/1‑136 (MQ=255) tttttttttATTTTCTATGATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGCAATTGCTAGTTGACCtt < 1:1135045/136‑1 (MQ=255) | ‑‑‑‑‑‑GATAATTTCGTAGAGTATCGGGTGCGACCGATGC‑TTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGCAATTGCTAGTTGACCTT > TrimmedAssembly_T66_contig_9/1‑171 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |