| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | TrimmedAssembly_T66_contig_1 | 88,020 | G→A | A159A (GCC→GCT) | rasttk_feature_annotation_tool=annotate_proteins_km er_v2 ← | Transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | TrimmedAssembly_T66_contig_1 | 88,020 | 0 | G | A | 96.5% | 192.4 / 1.5 | 85 | A159A (GCC→GCT) | rasttk_feature_annotation_tool=annotate_proteins_km er_v2 | Transposase |
| Reads supporting (aligned to +/- strand): ref base G (0/3); new base A (39/43); total (39/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TGATTCCGTCGGCGCCACTTGGTAGTTCAGCACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATGGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCTTTAGTCATGTTGGAAACCGTGGCTTT > TrimmedAssembly_T66_contig_1/87888‑88106 | tGATTCCGTCGGCGCCACTTGGTAGTTCAGCACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGcc < 2:174753/136‑1 (MQ=21)tGATTCCGTCGGCGCCACTTGGTAGTTCAGCACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGcc < 1:1848118/136‑1 (MQ=21)tGATTCCGTCGGCGCCACTTGGTAGTTCAGCACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGcc > 1:690781/1‑136 (MQ=21)tGATTCCGTCGGCGCCACTTGGTAGTTCAGCACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGc < 1:2286737/135‑1 (MQ=21) gATTCCGTCGGCGCCACTTGGTAGTTCAGCACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCg < 1:920149/136‑1 (MQ=21) gATTCCGTCGGCGCCACTTGGTAGTTCAGCACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCg < 1:920153/136‑1 (MQ=21) gATTCCGTCGGCGCCACTTGGTAGTTCAGCACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCg < 1:1552627/136‑1 (MQ=21) aTTCCGTCGGCGCCACTTGGTAGTTCAGCACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGc > 2:1295887/1‑136 (MQ=21) cGTCGGCGCCACTTGGTAGTTCAGCACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCatat < 1:2303819/136‑1 (MQ=21) gTCGGCGCCACTTGGTAGTTCAGCACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGAGGCATCTAATAAGAAAGCCGCatat > 1:429786/1‑135 (MQ=255) gcgcCACTTGGTAGTTCAGCACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGa > 1:1858090/1‑136 (MQ=17) aGTTCAGCACTTCTTTCGTACCATCTGGACGAATGCTAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACg > 2:636273/1‑136 (MQ=17) cAGCACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGc < 2:194695/136‑1 (MQ=17) aGCACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGtt > 2:1123435/1‑133 (MQ=17) aGCACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGtt > 1:1499705/1‑133 (MQ=17) cACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTgg > 1:899467/1‑136 (MQ=17) cACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTgg > 1:1731553/1‑136 (MQ=17) cACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTgg > 2:1921243/1‑136 (MQ=17) cACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTgg > 2:2359541/1‑136 (MQ=17) cACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTgg > 2:81768/1‑136 (MQ=17) aCTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCt < 2:2247719/133‑1 (MQ=17) aCTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCt < 2:1093253/133‑1 (MQ=17) aCTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCt < 1:1322662/133‑1 (MQ=17) aCTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCt < 2:690781/133‑1 (MQ=17) aCTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCt < 1:1081712/133‑1 (MQ=17) aCTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCt < 1:221737/133‑1 (MQ=17) aCTTCTTTCGTACCATCTGGAAGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTTGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCt < 1:1106878/133‑1 (MQ=12) ttcttTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGaaa > 2:943840/1‑136 (MQ=17) ttcttTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGaaa > 2:2452489/1‑136 (MQ=17) ttcttTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGaaa > 2:734995/1‑136 (MQ=17) cttTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGc < 2:2075534/136‑1 (MQ=17) tCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGaaa < 2:1765431/131‑1 (MQ=17) tCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGaaa > 1:1765431/1‑131 (MQ=17) tCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTg > 1:2329281/1‑136 (MQ=17) tCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTg > 2:789728/1‑136 (MQ=17) tCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTg > 2:166027/1‑136 (MQ=17) tCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTg < 1:1678689/136‑1 (MQ=17) tCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTg > 1:460379/1‑136 (MQ=17) tCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTg > 1:1414449/1‑136 (MQ=17) tCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTg < 1:1929447/136‑1 (MQ=17) ccATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCt < 1:414526/121‑1 (MQ=17) ccATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCt > 2:414526/1‑121 (MQ=17) ccATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGaa > 1:835257/1‑133 (MQ=17) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGcc > 1:2052213/1‑87 (MQ=18) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGcc < 2:2052213/87‑1 (MQ=18) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATCTTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCAt > 2:2249343/1‑136 (MQ=17) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAATCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCAt > 1:1063684/1‑136 (MQ=17) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAAcc > 1:1147107/1‑129 (MQ=17) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAAcc < 2:1147107/129‑1 (MQ=17) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCAt < 2:2542454/136‑1 (MQ=17) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCAt < 2:396091/136‑1 (MQ=17) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCAt < 1:1409605/136‑1 (MQ=17) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCAt > 2:37502/1‑136 (MQ=17) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCAt < 2:409653/136‑1 (MQ=17) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCAt > 2:1140902/1‑136 (MQ=17) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCAt < 1:1921243/136‑1 (MQ=17) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCAt < 1:2039684/136‑1 (MQ=17) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCAt < 1:633141/136‑1 (MQ=17) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCAt < 1:1123435/136‑1 (MQ=17) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCAt > 1:2545984/1‑136 (MQ=17) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCAt < 2:872490/136‑1 (MQ=17) ggACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCGTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCAt > 1:1056630/1‑136 (MQ=17) cGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGa < 1:2092359/136‑1 (MQ=17) aTGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACg < 1:2529411/135‑1 (MQ=17) gCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCt > 1:2406579/1‑136 (MQ=17) ccAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCtt > 2:2514697/1‑136 (MQ=17) cAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCttt > 1:1768789/1‑136 (MQ=17) aaTCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATATCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCTTTa < 2:479648/136‑1 (MQ=17) aaTCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCTTTa < 2:2559362/136‑1 (MQ=17) aaTCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCTTTa < 1:1516282/136‑1 (MQ=17) aaTCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCTTTa < 1:1883355/136‑1 (MQ=17) aaTCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTAGAACCTGTTCATTGACGGCTTTa < 2:2559371/136‑1 (MQ=17) cGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCTTTAGTc > 1:2057796/1‑136 (MQ=17) cGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGAAGGCTTTAGTc > 1:2057736/1‑136 (MQ=17) gCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCTTTAGTCa > 2:375734/1‑136 (MQ=17) gCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCTTTAGTCa > 1:1283549/1‑136 (MQ=17) aaTATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCTTTAGTCATg < 1:1217663/136‑1 (MQ=17) tGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCTTTAGTCATGTTgg > 2:474447/1‑136 (MQ=17) aCCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCTTTAGTCATGTTGGaaa < 1:2249343/136‑1 (MQ=17) aCCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCTTTAGTCATGTTGGaaa < 2:460379/136‑1 (MQ=17) aCCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCTTTAGTCATGTTGGaaa < 2:2479181/136‑1 (MQ=17) ccGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATAGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCTTTAGTCATGTTGGAAAc < 2:2364640/136‑1 (MQ=14) ttatttttGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATGGCCGCATAGTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGGTCATTGACGGCTTTAGTCATGTTGGAAACCGTg < 2:300869/133‑1 (MQ=0) tttgctcGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATGGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCTTTAGTCATGTTGGAAACCGTGGCttt < 2:2466222/130‑1 (MQ=1) tgtgcaCGGTATCCCGCTTTACCGGCAAGTAAGTGGCATCTAAGAAGATGGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCTTTAGTCATGTTGGAAACCGTGGCttt < 2:700262/131‑1 (MQ=0) | TGATTCCGTCGGCGCCACTTGGTAGTTCAGCACTTCTTTCGTACCATCTGGACGAATGCCAATCGCAATATGAACCGCTTCTTTTTGAACGGTATCCCGCTTTAACGGCAAGTAAGTGGCATCTAAGAAGATGGCCGCATATTGTGAAGCCAGTCGACGTTGCTGGAAAGCTTGAACCTGTTCATTGACGGCTTTAGTCATGTTGGAAACCGTGGCTTT > TrimmedAssembly_T66_contig_1/87888‑88106 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |