| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | TrimmedAssembly_T66_contig_9 | 36 | T→C | intergenic (–/+223) | – / ← rasttk_feature_annotation_tool=elepa@bvbrc | –/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | TrimmedAssembly_T66_contig_9 | 36 | 0 | T | C | 80.2% | 809.1 / 189.4 | 339 | intergenic (–/+223) | –/rasttk_feature_annotation_tool=elepa@bvbrc | –/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (36/31); new base C (129/142); total (166/173) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01 | |||||||||||
Only 100 of 343 total aligned reads displayed.
‑‑‑‑‑GATAATTTCGTAGAGTATCGGGTGCGACCGATGCTTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGCAATTGCTAGTTGACCTT > TrimmedAssembly_T66_contig_9/1‑171 | tcgaggataatTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcg < 1:975416‑M2/131‑54 (MQ=255)tcgaggataatTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttc < 1:227528‑M2/108‑31 (MQ=255)tcgaggataatTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctct > 2:2165810‑M2/6‑76 (MQ=255)tcgaggataatTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctct > 1:1485063‑M2/6‑76 (MQ=255) cgaggataaTTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctctt > 2:1949904‑M2/5‑75 (MQ=255) cgaggataaTTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctctt > 2:1072437‑M2/5‑75 (MQ=255) cgaggATAATTTCGTAGAGTATCGGGTGCGACCGATGCTTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCctct < 2:969625/132‑1 (MQ=255) aggatAATTTCGTAGAGTATCGGGTGCGACCGATGCTTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcaca < 2:1898777‑M2/126‑48 (MQ=255) aggatAATTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAATACAGGTCTGGAAAAATCCGAAGATTTTTCAAGACCTGTTTTGTTTCTAGAGACTTATTTCACAGCCTCTtgt < 2:2216113‑M2/134‑4 (MQ=255) aggatAATTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGgaaaaatccgaa > 2:2219188‑M2/3‑73 (MQ=255) aggatAATTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgtat < 2:945306‑M2/134‑57 (MQ=255) aggatAATTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctctcta < 2:2286134‑M2/134‑64 (MQ=255) aggatAATTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgt < 1:1224803‑M2/134‑4 (MQ=255) aggATAATTTCGTAGAGTATCGGGTGCGACCGATGC‑TCTTTTTTTATTTTCTATTATATCAAAAACa > 1:594351/3‑67 (MQ=255) ggATAATTTCGTAGATTATCGGGTGCGACCGATGCTTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTc < 1:770055/134‑1 (MQ=255) aTAATTTCGTAGAGTATCGGGTGCGACCGATGCTTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCg < 1:2445696/134‑1 (MQ=255) tAATTTCGTAGATTATCGGGTGCGACCGATGCTCTTTTTTTT‑TTTTCTATTATATCAAAAACAGGTATGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgttttt > 1:73633‑M2/1‑129 (MQ=255) tAATTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttt > 2:814026‑M2/1‑76 (MQ=255) tAATTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttt > 2:741030‑M2/1‑76 (MQ=255) tAATTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcttttttg > 1:567063‑M2/1‑69 (MQ=255) tAATTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgttttt > 2:442571‑M2/1‑129 (MQ=255) tAATTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgttttt > 2:2738234‑M2/1‑129 (MQ=255) aaTTTCGTAGAGTATCGGGTGCGACCGATGCTTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTc > 2:1584119/1‑131 (MQ=255) aaTTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTGTTATATCATAAGCAGGTCTGGAAAATTtcgaagatttttccaggcctgcgttgtgtctagtgacttttgtcacagcctcggatgggtt < 2:2246162‑M2/136‑62 (MQ=255) aaTTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttata < 1:469149‑M2/136‑62 (MQ=255) aaTTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgttttttta > 1:880832‑M2/1‑68 (MQ=255) aaTTTCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgtttttg > 1:2670912‑M2/1‑128 (MQ=255) aTTTCGTAGAGTATCGGGTGCGACCGATGCTTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTAttt < 2:1341916/136‑1 (MQ=255) aTTTCGTAGAGTATCGGGTGCGACCGATGCTTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTAttt < 1:320509/136‑1 (MQ=255) ttCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcttttttgttcta > 2:1349868‑M2/1‑65 (MQ=255) ttCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcttttttgttcta > 1:110617‑M2/1‑65 (MQ=255) ttCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcttttttgttcta > 1:1689053‑M2/1‑65 (MQ=255) ttCGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcttttttgttcta > 1:523389‑M2/1‑65 (MQ=255) cGTAGAGTATCGTTTTCGACCTATGATTTTTTTTTTATTTTATATTATATCAATAGCAGGTCTTCAAAAATCGGAAGATTTTTCAAGACCTGATTGTTTGCTAGAGACTTATGTCACAGCCTCTTCttaattaaaa > 2:2020035/1‑134 (MQ=19) cGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattact > 1:2189235‑M2/1‑63 (MQ=255) cGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattact > 1:1368500‑M2/1‑63 (MQ=255) cGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattact > 1:282846‑M2/1‑63 (MQ=255) cGTAGAGTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattact > 2:1850343‑M2/1‑63 (MQ=255) cGTAGAGTATCGGGTGCGACCGATGCACTTTTTTTC‑TTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattact > 2:465695‑M2/1‑63 (MQ=255) agagTATCGGGTGCGACCGATGCTTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACt < 1:1593087/136‑1 (MQ=255) agagTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttataaaaaatat < 1:2000465‑M2/136‑70 (MQ=255) agagTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttttgaaagtgtt < 1:2142821‑M2/136‑70 (MQ=255) gagTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagtcctgttttgtttatagagacttatgtcacagcctcgtttttttattacacatc < 2:842947‑M2/136‑78 (MQ=255) agTATCGGGTGCGACCGATGCTTTTTTTTTTAtttt > 2:533796/1‑36 (MQ=255) agTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTGTATTATATCAAAATCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgtatgggttaatcgaaaaa < 1:2213108‑M2/136‑72 (MQ=255) agTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttataaaaaatattt < 1:1307682‑M2/136‑72 (MQ=255) agTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttct < 1:2329454‑M2/96‑32 (MQ=255) agTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttct > 2:2329454‑M2/1‑65 (MQ=255) agTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctctt > 2:287225‑M2/1‑58 (MQ=255) agTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctctt < 1:287225‑M2/119‑62 (MQ=255) agTATCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTATATACTTATGTCACAGCCTCTtgtttttgatgtctatta < 1:2530360‑M2/136‑19 (MQ=255) aTCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGgaaaaatccgaagattt > 2:148564‑M2/1‑55 (MQ=255) aTCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgtatgggttaatcgaaaaat > 2:2544457‑M2/1‑62 (MQ=255) aTCGGGTGCGACCGATGCTCTTTTTTT‑ATTTTATATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttttgaaagtgttagtt > 1:615164‑M2/1‑62 (MQ=255) cGGGTGCGACCTATGCTTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGc < 2:2516940/136‑1 (MQ=255) cGGGTGCGACCGATGCTTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGc > 2:558209/1‑136 (MQ=255) cGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttttgaaagtgttagttcgt < 1:1208207‑M2/136‑77 (MQ=255) cGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattactcatcgttatt > 1:1141014‑M2/1‑53 (MQ=255) cGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattactcatcgttatt > 1:2560563‑M2/1‑53 (MQ=255) cGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgtttttgatgtctattaaaatg < 1:177566‑M2/136‑24 (MQ=255) cGGGTGCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgtttttgatgt < 2:2457766‑M2/125‑13 (MQ=255) cGGGTGCGACCGATGC‑TCTTTTTTTATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGAttttt < 1:40814/72‑1 (MQ=38) gTGCGACCGATGCTTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGCaa < 1:2367715/135‑1 (MQ=255) gCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAATAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttataaaaaatatttaaatgtcttg > 2:172769‑M2/1‑55 (MQ=255) gCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttataaaaaatatttaaatgtctt < 1:857771‑M2/135‑81 (MQ=255) gCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttataaaaaatatttaaatgtctt > 1:1635313‑M2/1‑55 (MQ=255) gCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATccgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgcctttttgaaagtgttagttcgttgacg > 2:2693586‑M2/1‑55 (MQ=255) gCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattactcatcgttattaaac > 2:61265‑M2/1‑48 (MQ=255) gCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattactcatcgttattaaac > 1:1565415‑M2/1‑48 (MQ=255) gCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattactcatcgttattaaac > 1:718715‑M2/1‑48 (MQ=255) gCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattactcatcgttattaaac > 1:1004613‑M2/1‑48 (MQ=255) gCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgttt > 2:2429544‑M2/1‑48 (MQ=255) gCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgtttttgatgtctattaaaatggaccc > 2:796210‑M2/1‑108 (MQ=255) gCGACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgtttttgatgcctattaaaatggaccc > 1:1747006‑M2/1‑108 (MQ=255) gCGACCGAGGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagaattatgtcacagcctcgtatgggttaatcgaaaaattctttatat < 1:2494965‑M2/136‑82 (MQ=255) gACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttataaaaaatatttaaatgtcttgc > 1:2219819‑M2/1‑53 (MQ=255) gACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgtatgggttaatcgaaaaattctttatata > 2:1124097‑M2/1‑53 (MQ=255) gACCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattactcatcgttattaaacaa > 1:2605965‑M2/1‑46 (MQ=255) aCCGATGCTTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGCAATTGCTa > 1:1532206/1‑136 (MQ=255) aCCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttataaaaaatatttaaatgtcttgcaa < 1:2012426‑M2/136‑85 (MQ=255) aCCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttataaaaaatatttaaatgtcttgcaa < 1:2781609‑M2/136‑85 (MQ=255) aCCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttataaaaaatatttaaatgtcttgcaa < 2:309457‑M2/136‑85 (MQ=255) aCCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttataaaaaatatttaaatgtatttcaa < 2:2124959‑M2/136‑85 (MQ=255) aCCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgtatgggttaatcgaaaaattctttatataac < 1:2709811‑M2/136‑85 (MQ=255) aCCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgtatg < 1:452347‑M2/109‑58 (MQ=255) aCCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACGGGTCTGgaaaaatccgaagatttttccagacctgttttgttt > 2:1414123‑M2/1‑45 (MQ=255) aCCGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgttttt < 1:2656937‑M2/110‑66 (MQ=255) ccGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttataaaaaatatttaaatgtcttgcaaa < 2:2825304‑M2/136‑86 (MQ=255) ccGATGC‑TCTTTTTTTATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGAttttt > 1:1546222/1‑63 (MQ=38) cGATGCTTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGCAATTGCTAGt > 2:1683812/1‑136 (MQ=255) cGATGCTTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGCAATTGCTAGt > 1:382736/1‑136 (MQ=255) cGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttataaaaaatatttaaatgtcttgcaaaa < 2:380190‑M2/136‑87 (MQ=255) cGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttataaaaaatatttaaatgtcttgcaaaa < 2:1785079‑M2/136‑87 (MQ=255) cGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcttttttataaaaaatatttaaatgtcttgcaaaa < 1:2028650‑M2/136‑87 (MQ=255) cGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATtcgaagatttttccagacctgctttgtttctagagacttatgtcacagcctcgtatgggttaatcgaaaaattctttatataacgc < 1:657758‑M2/136‑87 (MQ=255) cGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattactcatcgttattaaacaatcat < 2:1561500‑M2/136‑94 (MQ=255) cGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattactcatcgttattaaacaatcat < 1:1959492‑M2/136‑94 (MQ=255) cGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGgaaaaatccgaagatttttccagacctgttttgtttctagagacttatgtcacagcctcgtttttttattactcatcgttattaaacaatcat < 1:1890393‑M2/136‑94 (MQ=255) cGATGCTCTTTTTTT‑ATTTTCTATTATATCAAAAACAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGTTTTGTTTCTAGAGACTTATGTCACAGCCTCTtgtttttgatgtctattaaaatggaccctaccg < 2:2784140‑M2/136‑34 (MQ=255) tttttttttATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGCAATTGCTAGTTGACCtt < 2:2092253/136‑1 (MQ=255) | ‑‑‑‑‑GATAATTTCGTAGAGTATCGGGTGCGACCGATGCTTTTTTTTTTATTTTCTATTATATCAAAAGCAGGTCTGGAAAAATCCGAAGATTTTTCCAGACCTGCTTTGTTTCTAGAGACTTATGTCACAGCCTCTTCGTATTTAACAACTGCATTGCAATTGCTAGTTGACCTT > TrimmedAssembly_T66_contig_9/1‑171 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |