| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | Annotation_U21_Trimmed_contig_1 | 64,267 | T→C | S371P (TCA→CCA) | rasttk_feature_annotation_tool=annotate_proteins_km er_v2 → | RNA polymerase sigma factor RpoD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Annotation_U21_Trimmed_contig_1 | 64,267 | 0 | T | C | 99.6% | 1163.7 / NA | 281 | S371P (TCA→CCA) | rasttk_feature_annotation_tool=annotate_proteins_km er_v2 | RNA polymerase sigma factor RpoD |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (154/126); minor base A (0/1); total (154/127) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 281 total aligned reads displayed.
CGTTTGCGCTTTGGATTAGATGATGGACGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGATCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACGAAAGATAGTAGGAGTTTATTTTATGAA > Annotation_U21_Trimmed_contig_1/64135‑64396 | cTTTGGATTAGATGATGGACGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACt > 2:384616/1‑135 (MQ=255) aTTAGATGATGGACGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGa > 1:2245123/1‑135 (MQ=255) aTTAGATGATGGACGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGa > 2:1804404/1‑135 (MQ=255) gatgGACGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCt < 1:210009/134‑1 (MQ=255) gatgGACGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCt < 1:1820689/134‑1 (MQ=255) atgGACGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACTTAAACTCCTCCATCCATCAAGACCAAAACAACTAAAAGAATTTATAg > 1:67327/1‑135 (MQ=255) gACGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGAtt < 2:1673883/135‑1 (MQ=255) gACGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGAtt < 2:2351202/135‑1 (MQ=255) gACGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGAtt < 2:1157579/135‑1 (MQ=255) aCGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAGCGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTa > 1:706937/1‑135 (MQ=255) aCGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTa > 1:486200/1‑135 (MQ=255) aCGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTa > 2:2125479/1‑135 (MQ=255) aCGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTa > 2:495559/1‑135 (MQ=255) aCGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTa > 1:159295/1‑135 (MQ=255) aCGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTa > 1:1528116/1‑135 (MQ=255) aCGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTa > 1:1312699/1‑135 (MQ=255) cGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTa < 1:431669/134‑1 (MQ=255) cGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTa < 2:1971233/134‑1 (MQ=255) tACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGtt < 2:2521039/135‑1 (MQ=255) tACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGtt < 2:1489155/135‑1 (MQ=255) gAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAAccc < 2:1312699/135‑1 (MQ=255) gAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAAccc < 2:2302181/135‑1 (MQ=255) tctTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAaga > 2:2055103/1‑135 (MQ=255) tctTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTACTAGATTAGTTAACACAaga > 1:2346621/1‑135 (MQ=255) tGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAgg > 2:1720550/1‑134 (MQ=255) aagaagTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGtt < 1:1384622/121‑1 (MQ=255) aagaagTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTc > 1:944569/1‑135 (MQ=255) aagaagTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTc > 1:88364/1‑135 (MQ=255) aagaagTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTc > 1:509909/1‑135 (MQ=255) aagaagTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTc > 1:2757313/1‑135 (MQ=255) aagaagTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTc > 2:29107/1‑135 (MQ=255) aagaagTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTc > 2:668140/1‑135 (MQ=255) aagaagTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTc > 1:1285899/1‑135 (MQ=255) aagaagTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTc > 2:702656/1‑135 (MQ=255) aagaagTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTc > 2:924203/1‑135 (MQ=255) aagaagTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTc > 1:1015080/1‑135 (MQ=255) aagaagTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTAAGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGTTTTTCTAGATTAGTTAAACCAAGAGGTTc > 2:800706/1‑135 (MQ=255) gaagTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTcc < 2:1396867/134‑1 (MQ=255) gaagTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCg < 2:282705/135‑1 (MQ=255) gaagTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCg > 2:94696/1‑135 (MQ=255) gTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAATAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGt < 1:52180/133‑1 (MQ=255) gTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGt < 1:2112378/133‑1 (MQ=255) gttAAAGTATTTGGAGTTACTAGAGAACGTATTCGTAAAATTGAAGCGTAATCTTTACTTAAACTCCGCCATCCATCAAGACCTAAACACCTAAAAGATTTTCTAGATTAGTTATCCCACGAGGTTCCGTGCAgg < 1:2831330/133‑1 (MQ=255) ggTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAAccc < 2:1901319/118‑1 (MQ=255) ggTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAAccc > 1:1901319/1‑118 (MQ=255) ggTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGc < 1:2770257/132‑1 (MQ=255) ggTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGc > 2:2770257/1‑132 (MQ=255) ggTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAg > 2:2646931/1‑134 (MQ=255) tAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTc > 1:1107863/1‑135 (MQ=255) tAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTc > 1:2392583/1‑135 (MQ=255) tAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTc > 1:2372931/1‑135 (MQ=255) aGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTa < 1:2165068/135‑1 (MQ=255) aGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTa < 1:2026724/135‑1 (MQ=255) aTTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGAt > 2:2877377/1‑135 (MQ=255) ttGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGAttt > 1:585237/1‑135 (MQ=255) tGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTtg < 2:1527637/135‑1 (MQ=255) gAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTtgtg > 2:718864/1‑135 (MQ=255) aaGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCTTAGCTGTGTGCAGGAcc > 2:1208168/1‑135 (MQ=255) aaGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGAcc > 2:2699993/1‑135 (MQ=255) aaGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGAcc > 1:1677785/1‑135 (MQ=255) aaGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGAcc > 2:768550/1‑135 (MQ=255) aaGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGAcc > 1:1011201/1‑135 (MQ=255) aaGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGAcc > 2:1595787/1‑135 (MQ=255) aaGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGAcc > 1:643179/1‑135 (MQ=255) cGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCttttttt < 1:307898/135‑1 (MQ=255) cGTATTCGTCAAATTGAAGCAAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCttttttt > 1:1729075/1‑135 (MQ=255) gTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTg > 1:2632309/1‑135 (MQ=255) aTTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGaa > 1:2033330/1‑135 (MQ=255) aTTCGTCAAATTGAAGCGAAAGCTTTACGAAAACTCAGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGAAGATTTGTGCAGGACCTTTATTTGaa > 1:137573/1‑135 (MQ=255) ttCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGaa < 2:2593325/134‑1 (MQ=255) cGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGAtt < 1:741117/76‑1 (MQ=255) cGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCttttt < 2:2415120/127‑1 (MQ=255) cGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGa < 2:819929/135‑1 (MQ=255) cGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGa > 2:847190/1‑135 (MQ=255) cGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGa > 2:1299585/1‑135 (MQ=255) tCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGata > 2:222796/1‑135 (MQ=255) tCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGata > 2:434270/1‑135 (MQ=255) aaTTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGatat > 2:2479282/1‑133 (MQ=255) aTTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGaaa < 1:1631668/127‑1 (MQ=255) aTTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGaaa > 2:1631668/1‑127 (MQ=255) gCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATaa < 1:384616/134‑1 (MQ=255) gAAAGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTa > 1:1837815/1‑135 (MQ=255) aGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACg < 1:635241/134‑1 (MQ=255) aGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACg < 2:892171/134‑1 (MQ=255) aGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACg < 1:1715848/134‑1 (MQ=255) aGCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACg < 2:1285899/134‑1 (MQ=255) gCTTTACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACGaa > 2:2596144/1‑135 (MQ=255) tttACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACGAAAg > 2:2006230/1‑135 (MQ=255) ttACGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTATAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACGAAAGa < 1:2658745/135‑1 (MQ=255) aCGTAAACTCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACGAAAGAta < 2:1114315/135‑1 (MQ=255) tCCGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACGAAAGATAGTAGGAGt < 2:88364/135‑1 (MQ=255) ccGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACGAAAGATAGTAGGAGtt > 1:1881783/1‑135 (MQ=255) ccGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACGAAAGATAGTAGGAGtt > 1:2726448/1‑135 (MQ=255) ccGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACGAAAGATAGTAGGAGtt > 2:1193691/1‑135 (MQ=255) ccGCCATCCATCAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACGAAAGATAGTAGGAGtt > 1:1023183/1‑135 (MQ=255) atccatcAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACGAAAGATAGTAGGAGtttattt > 1:1234632/1‑135 (MQ=255) tccatcAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACGAAAGATAGTAGGAGtttatttt < 2:105857/135‑1 (MQ=255) catcAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACGAAAGATAGTAGGAGtttattttat < 1:255631/135‑1 (MQ=255) tcAAGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACGAAAGATAGTAGGAGTTTATTTTATGa < 1:897379/135‑1 (MQ=255) aaGACCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACGAAAGATAGTAGGAGTTTATTTTATGaa > 1:971091/1‑134 (MQ=255) | CGTTTGCGCTTTGGATTAGATGATGGACGTACTAGAACTCTTGAAGAAGTTGGTAAAGTATTTGGAGTTACAAGAGAACGTATTCGTCAAATTGAAGCGAAAGCTTTACGTAAACTCCGCCATCCATCAAGATCAAAACAACTAAAAGATTTTCTAGATTAGTTAACCCAAGAGGTTCCGTGCAGGTCGTAGATTTGTGCAGGACCTTTTTTTGAAAGATATAACTATAATTACGAAAGATAGTAGGAGTTTATTTTATGAA > Annotation_U21_Trimmed_contig_1/64135‑64396 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |