Predicted mutation
evidence seq id position mutation annotation gene description
RA Annotation_U21_Trimmed_contig_4 75,126 T→C C31R (TGC→CGC)  rasttk_feature_annotation_tool=annotate_proteins_km er_v2 → Replication‑associated recombination protein RarA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Annotation_U21_Trimmed_contig_475,1260TC99.3% 1202.8 / NA 294C31R (TGC→CGC) rasttk_feature_annotation_tool=annotate_proteins_km er_v2Replication‑associated recombination protein RarA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (104/188);  minor base A (0/2);  total (104/190)
Fisher's exact test for biased strand distribution p-value = 5.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

TTTTTTATTTACTTTAAATTTTTATTTTAGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACTGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGAACGCTGCCACCG  >  Annotation_U21_Trimmed_contig_4/74998‑75255
                                                                                                                                |                                                                                                                                 
  ttttATTTACTTTAAATTTTTATTTTAGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTAACGAAGAAATTGTTTGTCAACATCATTTGGTCGGTCCTGGAAAAAATATATACCGCATGGtt                                                                                                                           >  1:1490387/1‑135 (MQ=255)
   tttatttaCTTTAAATTTTTATTTTAGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTa                                                                                                                          >  2:2524474/1‑135 (MQ=255)
   tttatttaCTTTAAATTTTTATTTTAGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTa                                                                                                                          >  2:1066459/1‑135 (MQ=255)
    ttatttaCTTTAAATTTTTATTTTAGGAGAAAAAATGCAACCAATAGCGTTTCGTATGCGCCCACCAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTaa                                                                                                                         >  1:1460544/1‑135 (MQ=255)
      ttttaCTTTAAATTTTTATTTTAGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAg                                                                                                                       <  1:594711/134‑1 (MQ=255)
      atttaCTTTAAATTTTTATTTTAGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAg                                                                                                                       <  1:1555768/135‑1 (MQ=255)
         taCTTTAAATTTTTATTTTAGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCa                                                                                                                    <  2:2459338/135‑1 (MQ=255)
              tAAATTTTTATTTTAGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACtt                                                                                                               <  2:1853310/135‑1 (MQ=255)
                  tttttATTTTAGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTAAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTat                                                                                                           <  2:1629636/135‑1 (MQ=255)
                    tttattttaGGAGAAAAAATGCAACCACTTGCGTTTCGTATTCGCCCACGATCTTTCGTACAAATTGGTGGTAAACAGCATTTGGTCGTTCCTTGAAAATTTATCGACCGCATGGTTAAAGCCAAACTTTTatca                                                                                                         <  2:1786997/135‑1 (MQ=255)
                     ttattttaGGAGAAAAATTGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCTAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGTAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTatcat                                                                                                        <  1:2057269/135‑1 (MQ=255)
                     ttattttaGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCGTGGTTAAAGCCAAACTTTTatcat                                                                                                        <  2:229148/135‑1 (MQ=255)
                     ttattttaGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTatcat                                                                                                        <  2:534853/135‑1 (MQ=255)
                     ttattttaGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTatcat                                                                                                        <  2:2147147/135‑1 (MQ=255)
                     ttattttaGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTatcat                                                                                                        <  1:1970319/135‑1 (MQ=255)
                     ttattttaGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTatcat                                                                                                        <  2:2487139/135‑1 (MQ=255)
                     ttattttaGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTatcat                                                                                                        <  1:287190/135‑1 (MQ=255)
                         tttaGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCAt                                                                                                    <  2:1051991/135‑1 (MQ=255)
                         tttaGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCAt                                                                                                    >  1:2479304/1‑135 (MQ=255)
                                gaAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGAtttt                                                                                              <  2:1163831/134‑1 (MQ=255)
                                gaAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGAtttt                                                                                              <  1:18676/134‑1 (MQ=255)
                                   aaaaTGCAACCATTAGCTTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATAc                                                                                          <  1:1649631/135‑1 (MQ=255)
                                   aaaaTGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTtata                                                                                           <  1:2218120/134‑1 (MQ=255)
                                   aaaaTGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATAc                                                                                          <  2:2080595/135‑1 (MQ=255)
                                   aaaaTGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATAc                                                                                          <  1:1180748/135‑1 (MQ=255)
                                   aaaaTGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATAc                                                                                          <  1:1354125/135‑1 (MQ=255)
                                   aaaaTGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATAc                                                                                          <  2:863394/135‑1 (MQ=255)
                                   aaaaTGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATAc                                                                                          <  2:1994695/135‑1 (MQ=255)
                                   aaaaTGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATAc                                                                                          <  2:239796/135‑1 (MQ=255)
                                   aaaaTGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATAc                                                                                          <  2:685267/135‑1 (MQ=255)
                                   aaaaTGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATAc                                                                                          <  2:1082678/135‑1 (MQ=255)
                                    aaaTGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACg                                                                                         <  2:1728158/135‑1 (MQ=255)
                                            ccATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAAGTATCGACCGCATGGTTATAGCCAAACTTTTATCATCCAGGAGTTTATACGGGcctcct                                                                                 >  2:32796/1‑135 (MQ=255)
                                             cATTCGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTg                                                                                <  1:1395457/135‑1 (MQ=255)
                                             cATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTg                                                                                <  2:566437/135‑1 (MQ=255)
                                             cATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTg                                                                                <  2:618874/135‑1 (MQ=255)
                                             cATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTg                                                                                <  2:795725/135‑1 (MQ=255)
                                             cATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTg                                                                                <  2:713982/135‑1 (MQ=255)
                                             cATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTg                                                                                <  1:680432/135‑1 (MQ=255)
                                                  gCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACt                                                                           <  1:1005253/135‑1 (MQ=255)
                                                  gCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACt                                                                           <  2:1490492/135‑1 (MQ=255)
                                                       tCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTACAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTa                                                                       <  1:989570/134‑1 (MQ=255)
                                                       tCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTa                                                                       <  1:1636041/134‑1 (MQ=255)
                                                         gTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTaa                                                                      >  2:2465963/1‑133 (MQ=255)
                                                          tATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACttt                                                                                                              <  1:2312227/92‑1 (MQ=255)
                                                          tATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAAc                                                                   >  2:957490/1‑135 (MQ=255)
                                                          tATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAAc                                                                   >  1:962877/1‑135 (MQ=255)
                                                          tATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAAc                                                                   >  2:462512/1‑135 (MQ=255)
                                                           aTGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACt                                                                  >  2:1916471/1‑135 (MQ=255)
                                                           aTGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACt                                                                  >  2:1367385/1‑135 (MQ=255)
                                                           aTGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACt                                                                  >  1:844601/1‑135 (MQ=255)
                                                            tGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACt                                                                  <  2:1824684/134‑1 (MQ=255)
                                                             gcgcCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAg                                                                >  2:278808/1‑135 (MQ=255)
                                                             gcgcCCACGAACAATCGAAAAAAATGATGGTCAACAGCATTTGGTCGGACCACGAAAAATTATCCACCGCATGGTTAAAGCCAAACTTTAATCATCCAAGAAAATAAACGTCCCTCCTGCAAATAGTAAAACTAg                                                                >  2:2369341/1‑135 (MQ=255)
                                                                cccACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTTGAAAAATTATCGACCGCATGGTTAAAGCCATACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTAt                                                             <  1:1162338/135‑1 (MQ=255)
                                                                cccACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTAt                                                             <  2:2433346/135‑1 (MQ=255)
                                                                cccACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTGAAAGCCAAACTTTTATCATCCATGATTTTATTCGGCCCTCCTGGAACTGGTAAAACTAGTAt                                                             <  2:1099221/135‑1 (MQ=255)
                                                                     gAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCa                                                        >  1:865131/1‑135 (MQ=255)
                                                                     caaCTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATTCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCa                                                        <  2:1040821/134‑1 (MQ=255)
                                                                       aCTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAg                                                       >  1:951934/1‑134 (MQ=255)
                                                                       aCTATCGAAGAAATTGTTGGTCAACAGCATGTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAg                                                       >  2:1390754/1‑134 (MQ=255)
                                                                              aagaaATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATAc                                                                                          <  1:1795892/92‑1 (MQ=255)
                                                                              aagaaATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTAttt                                               >  1:1780024/1‑135 (MQ=255)
                                                                              aagaaATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTAttt                                               >  2:2338839/1‑135 (MQ=255)
                                                                              aagaaATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTAttt                                               >  1:2258096/1‑135 (MQ=255)
                                                                              aagaaATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGACAGTGCTAttt                                               >  2:334771/1‑135 (MQ=255)
                                                                               agaaATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTc                                              >  2:663941/1‑135 (MQ=255)
                                                                               agaaATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTc                                              <  1:332898/135‑1 (MQ=255)
                                                                                taaaTTGTTGGTCAACAGCATTTGGTCGGTCCTGTAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTc                                              <  1:1864646/133‑1 (MQ=255)
                                                                                taaaTTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTc                                              <  1:405820/133‑1 (MQ=255)
                                                                                gaaATTGTTGGTCTACAGCATTTGGTCGTTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTTTACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTc                                              <  2:1560536/134‑1 (MQ=255)
                                                                                gaaATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTc                                              <  1:444636/134‑1 (MQ=255)
                                                                                gaaATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTc                                              <  1:2279025/134‑1 (MQ=255)
                                                                                   aTTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAg                                            <  1:1787841/133‑1 (MQ=255)
                                                                                   aTTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGc                                          <  1:1927028/135‑1 (MQ=255)
                                                                                      gttgGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTc                                        >  1:1733003/1‑134 (MQ=255)
                                                                                        tgGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAAc                                     >  1:2411547/1‑135 (MQ=255)
                                                                                                       ggtcggtcCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCATCTAAATATGCTTTTCg                      >  1:905091/1‑135 (MQ=255)
                                                                                                       ggtcggtcCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCg                      >  1:1227376/1‑135 (MQ=255)
                                                                                                       ggacggtcCTGGAAAAAGTATCGACCGCAGGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTTCAACTAGTAGTGCCCGTGCTAGTTCAGGCTCATCGAATTATGCTTGTCg                      >  2:2502925/4‑135 (MQ=255)
                                                                                                           ggtcCTGGAAAAATTATCGACCTCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAACTATGCTTTTCGAAtt                  <  2:1620222/135‑1 (MQ=255)
                                                                                                            gtcCTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAAttt                 >  1:1085777/1‑135 (MQ=255)
                                                                                                              ccTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAAtttt                <  2:1659078/134‑1 (MQ=255)
                                                                                                               cTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATAc                                                                                          <  1:2155176/59‑1 (MQ=255)
                                                                                                               cTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCTCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTg               >  1:832106/1‑134 (MQ=255)
                                                                                                               cTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTgg                                                                         <  1:25137/76‑1 (MQ=255)
                                                                                                               cTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAATCTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTg               >  2:974272/1‑134 (MQ=255)
                                                                                                               cTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTAt                                                             >  2:2569761/1‑88 (MQ=255)
                                                                                                               cTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTg               >  2:22654/1‑134 (MQ=255)
                                                                                                               cTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTg               >  2:1776377/1‑134 (MQ=255)
                                                                                                               cTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTg               >  1:1113186/1‑134 (MQ=255)
                                                                                                               cTGGAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCAATTTCAGGCTCAACTAAATATGCTTTTCGAAtttag               >  2:344789/1‑132 (MQ=255)
                                                                                                                 ggAAAAATTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGAAc            <  2:410159/135‑1 (MQ=255)
                                                                                                                    aaaaTTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGAACGc          >  1:103517/1‑134 (MQ=255)
                                                                                                                     aaaTTATCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGAtttt                                                                                              <  2:1456657/49‑1 (MQ=255)
                                                                                                                          aTCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGAACGCTGCCAcc   >  2:170813/1‑135 (MQ=255)
                                                                                                                          aTCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGAACGCTGCCAcc   >  2:1245736/1‑135 (MQ=255)
                                                                                                                          aTCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGAACGCTGCCAcc   >  1:624739/1‑135 (MQ=255)
                                                                                                                          aTCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGAACGCTGCCAcc   >  1:1951839/1‑135 (MQ=255)
                                                                                                                          aTCGACCGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGAACGCTGCCAcc   >  1:1377273/1‑135 (MQ=255)
                                                                                                                                |                                                                                                                                 
TTTTTTATTTACTTTAAATTTTTATTTTAGGAGAAAAAATGCAACCATTAGCGTTTCGTATGCGCCCACGAACTATCGAAGAAATTGTTGGTCAACAGCATTTGGTCGGTCCTGGAAAAATTATCGACTGCATGGTTAAAGCCAAACTTTTATCATCCATGATTTTATACGGCCCTCCTGGAACTGGTAAAACTAGTATTGCCAGTGCTATTTCAGGCTCAACTAAATATGCTTTTCGAATTTTGAACGCTGCCACCG  >  Annotation_U21_Trimmed_contig_4/74998‑75255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑